The effect of inhalation anesthetics on immunity of monocyte

• Project/Area Number: 24592370
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: National Defense Medical College
• Principal Investigator: Takahashi Tetsuya 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究, その他部局等, 講師 (00589905)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 敗血症 / 吸入麻酔薬 / 致死率 / ＬＰＳ / LPS

Outline of Final Research Achievements

Sevoflurane inhalation for either 3 or 6 h increased CO-Hb levels in mice and improved survival after LPS challenge concurrent with a significant suppression of TNF secretion. SnMP pretreatment suppressed sevoflurane-induced CO-Hb elevation. SnMP (0.1 mg/animal) completely abrogated the beneficial effect of sevoflurane on the survival of LPS-challenged mice and inhibited its suppressive effect on LPS-induced elevation of high-mobility group box 1 (HMGB1). However, SnMP did not inhibit the suppressive effect of sevoflurane on LPS-induced TNF secretion. Sevoflurane inhalation after LPS challenge also improved the survival of LPS-challenged mice, suggesting its clinical efficacy.
Sevoflurane inhalation may induce CO production and thereby improve the survival of LPS-challenged mice by reducing HMGB1 levels via the HO-1/CO pathway independent of TNF suppression.