| Project/Area Number |
24592376
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
宮崎 淳 筑波大学, 医学医療系, 准教授 (10550246)
西山 博之 筑波大学, 医学医療系, 教授 (20324642)
小島 崇宏 筑波大学, 医学医療系, 講師 (40626892)
島居 徹 筑波大学, 医学医療系, 教授 (80235613)
河合 弘二 筑波大学, 医学医療系, 講師 (90272195)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | gankyrin / testicular cancer / 精巣腫瘍 / ガンキリン / 奇形腫 / 悪性転化 / Rb |
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| Outline of Final Research Achievements |
We evaluated the expression of gankyrin, Rb and phosphorylated Rb (pRb) in human testis and testicular germ cell tumors by immunohistochemistry. The effects of suppression of gankyrin on phosphorylation status of Rb and cell proliferation were analyzed in vitro. Suppression of gankyrin led to suppression of Rb and cell proliferation in NEC8 cells. In testicular germ cell tumors, high expressions of gankyrin and pRb were observed in seminoma and embryonal carcinoma, whereas the expressions of both proteins were weak in histological subtypes of non-seminoma. Growing teratoma and testicular malignant transformation tissues expressed pRb strongly, but gankyrin faintly. Gankyrin is dominantly expressed in normal spermatocytes and seminoma/embryonal carcinoma, and its expression correlates well with pRb expression except in the growing teratoma and testicular malignant transformation cases. These data provide new insights into the molecular mechanisms of pathogenesis of testicular tumor.
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