Development of anticancer therapy with GGCT targeting siRNA for urothelial tumor
| Project/Area Number |
24592384
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HANADA Eiki 滋賀医科大学, 医学部, 助教 (40555067)
ARAKI Isao 滋賀医科大学, 医学部, 客員教授 (50252424)
YOSHIKI Tatsuhiro 滋賀医科大学, 医学部, 客員教授 (80230704)
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| Research Collaborator |
KAWAUCHI Akihiro 滋賀医科大学, 医学部, 教授 (90240952)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | γ-グルタミルシクロトランスフェラーゼ / RNA干渉 / 尿路上皮癌 / siRNA / GGCT / 癌治療 / C7orf24 |
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| Outline of Final Research Achievements |
We evaluated a utility of GGCT targeting therapy with RNAi for urothelial cancer.
In immunohistochemical analysis of surgical specimens of non-muscle invasive bladder cancer, high expression was revealed in 64% of the patients. The five-year recurrence free survivals of high and low-no GGCT expression groups were 30% and 57%, respectively. Significant antiprolifelative effect via GGCT targeting siRNA was shown in UMUC-3 cells, and combination treatment consisting of Adriamycin and GGCT siRNA reduced cell viability more than the treatment with Adriamycin alone. According to our results, GGCT can be a useful treatment target with RNAi for urothelial cancer. We continue the treatment experiment of tumor-bearing mice by administration GGCT siRNA, and evaluate therapeutic effects and adverse events.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Assessment of Sunitinib-Induced Toxicities and Clinical Outcomes Based on Therapeutic Drug Monitoring of Sunitinib for Patients With Renal Cell Carcinoma. Clin Genitourin Cancer.2015
Author(s)
Noda S, Otsuji T, Baba M, Yoshida T, Kageyama S, Okamoto K, Okada Y, Kawauchi A, Onishi H, Hira D, Morita SY, Terada T
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Journal Title
Clinical Genitourinary Cancer
Volume: in press
Issue: 4
Pages: 350-358
DOI
Related Report
Peer Reviewed
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