Identification and clinical application of novel biomarkers for neuroendocrine differentiation of prostate cancer

• Project/Area Number: 24592391
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Hiroshima University
• Principal Investigator: MATSUBARA Akio 広島大学, 医歯薬保健学研究院, 教授 (10239064)
• Co-Investigator(Kenkyū-buntansha): TEISHIMA Jun 広島大学, 医歯薬保健学研究院, 講師 (20397962) ; YASUI Wataru 広島大学, 医歯薬保健学研究院, 教授 (40191118) ; INOUE Shogo 広島大学, 病院, 助教 (90457177)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 前立腺癌 / 神経内分泌分化 / 去勢抵抗性 / RegIV / FGFR2

Outline of Final Research Achievements

Mechanism of molecules identified by CAST method in castration-resistance and neuroendocrine differentiation of prostate cancer cells have been investigated through cell-biological analysis, and the possibility as novel biomarkers of these molecules have been suggested through the experiments for expression in prostate cancer tissues and the measurement of serum concentration.

Research Products

• [Journal Article] FGF19 promotes progression of prostate cancer. (2015)
  • Author(s): Nagamatsu H, Teishima J, Goto K, Shikuma H, Kitano H, Shoji K, Inoue S, Matsubara A.
  • Journal Title: The Prostate Volume: Apr 8 Issue: 10 Pages: 1092-101
  • DOI: 10.1002/pros.22994
  • Peer Reviewed / Open Access
• [Journal Article] Restoration of fibroblast growth factor receptor 2IIIb enhances the chemosensitivity of human prostate cancer cells. (2014)
  • Author(s): Shoji K, Teishima J, Hayashi T, Ohara S, Mckeehan WL, Matsubara A.
  • Journal Title: Oncology Reports Volume: Jul;32(1) Issue: 1 Pages: 65-70
  • DOI: 10.3892/or.2014.3200
  • Peer Reviewed / Open Access
• [Journal Article] Expression of novel biomarkers identified by Escherichia coli ampicillin secretion trap: Oligophrenin-1 is associated with cell adhesion and migration in prostate cancer (2014)
  • Author(s): Goto K, Oue N, Hayashi T, Shinmei S, Sakamoto N, Sentani K, Teishima J, Matsubara A, Yasui W
  • Journal Title: Pathobiology Volume: 81 Issue: 4 Pages: 190-198
  • DOI: 10.1159/000363345
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of PRL1 as a novel diagnostic and therapeutic target for castration-resistant prostate cancer by the Escherichia coli ampicillin secretion trap (CAST) method (2014)
  • Author(s): Shunsuke Shinmei, Kazuhiro Sentani, Tetsutaro Hayashi, Naoya Sakamoto, Keisuke Goto, Htoo Zarni Oo, Yutaka Naito, Jun Teishima, Akio Matsubara, Naohide Oue, Hiroki Kuniyasu, Wataru Yasui
  • Journal Title: Urologic Oncology Volume: 32 Issue: 6 Pages: 769-778
  • DOI: 10.1016/j.urolonc.2014.03.007
  • Peer Reviewed / Open Access
• [Journal Article] Accumulation of FGF9 in prostate cancer correlates with epithelial-to-mesenchymal transition and induction of VEGF-A expression. (2014)
  • Author(s): Teishima J, Yano S, Shoji K, Hayashi T, Goto K, Kitano H, Oka K, Nagamatsu H, Matsubara A.
  • Journal Title: Anticancer Res Volume: Feb;34(2) Pages: 695-700
  • Peer Reviewed / Open Access
• [Journal Article] Prostate cancer detection by prostate-specific antigen-based screening in the Japanese Hiroshima area shows early stage, low-grade, and low rate of cancer-specific death compared with clinical detection. (2014)
  • Author(s): Teishima J, Maruyama S, Mochizuki H, Oka K, Ikeda K, Goto K, Nagamatsu H, Hieda K, Shoji K, Matsubara A.
  • Journal Title: Canadian Urology Assocciation Journal Volume: May;8(5-6) Issue: 5-6 Pages: 327-327
  • DOI: 10.5489/cuaj.1715
  • Peer Reviewed / Open Access
• [Journal Article] Psychological factor, metacognition, is associated with the advantage of suturing techniques acquired on a virtual reality simulator of robot-assisted surgery. (2014)
  • Author(s): Teishima J, Hattori M, Matsubara A.
  • Journal Title: Int J Urol. Volume: 21(3) Issue: 3 Pages: 349-50
  • DOI: 10.1111/iju.12286
  • Peer Reviewed
• [Journal Article] Accumulation of FGF9 in Prostate Cancer Correlates with Epithelial-to-Mesenchymal Transition and Induction of VEGF-A Expression. (2014)
  • Author(s): Teishima J, Yano S, Shoji K, Hayashi T, Goto K, Kitano H, Oka K, Nagamatsu H, Matsubara A.
  • Journal Title: Anticancer Res Volume: 34(2) Pages: 695-700
  • Peer Reviewed
• [Journal Article] The search for secreted proteins in prostate cancer by the Escherichia coli ampicillin secretion trap: expression of NBL1 is highly restricted to the prostate and is related to cancer progression. (2013)
  • Author(s): Hayashi T, Sentani K, Oue N, Ohara S, Teishima J, Anami K, Sakamoto N, Matsubara A, Yasui W.
  • Journal Title: Pathobiology Volume: 80 Issue: 2 Pages: 60-69
  • DOI: 10.1159/000341396
  • Peer Reviewed
• [Journal Article] MicroRNA-155 is a predictive marker for survival in patients with clear cell renal cell carcinoma. (2013)
  • Author(s): Shinmei S, Sakamoto N, Goto K, Sentani K, Anami K, Hayashi T, Teishima J, Matsubara A, Oue N, Kitadai Y, Yasui W.
  • Journal Title: International Journal of Urology Volume: 20 (5) Pages: 468-77
  • NAID: 10031177678
  • Peer Reviewed
• [Journal Article] STAT3 polymorphism can predict the response to interferon-α therapy in patients with metastatic renal cell carcinoma. (2013)
  • Author(s): Eto M, Kamba T, Miyake H, Fujisawa M, Kamai T, Uemura H, Tsukamoto T, Azuma H, Matsubara A, Nishimura K, Nakamura T, Ogawa O, Naito S; Japan Immunotherapy SNPs-Study Group for Kidney Cancer.
  • Journal Title: Eur Urol. Volume: 63 Issue: 4 Pages: 745-752
  • DOI: 10.1016/j.eururo.2012.09.052
  • Peer Reviewed
• [Journal Article] Expression of miR-486 is a potential prognostic factor after neophrectomy in advanced renal cell carcinoma (2013)
  • Author(s): Keisuke Goto, Naohide Oue, Shunsuke Shinmei, Kazuhiro Sentani, Naoya Sakamoto, Yutaka Naito, Tetsutaro Hauashi, Jun Teishima, Akio Matsubara, Wataru Yasui
  • Journal Title: Molecular and Clinical Oncology Volume: 1 Issue: 2 Pages: 235-240
  • DOI: 10.3892/mco.2012.46
  • Peer Reviewed
• [Journal Article] Restoration of IGFBP-rP1 increases radiosensitivity and chemosensitivity in hormone-refractory human prostate cancer. (2013)
  • Author(s): Seki M, Teishima J, Mochizuki H, Mutaguchi K, Yasumoto H, Oka K, Nagamatsu H, Shoji K, Matsubara A
  • Journal Title: Hiroshima J Med Sci 62(1) 13-19, 2013. Volume: 62(1)Mar Pages: 13-19
  • NAID: 120005323993
• [Journal Article] Impact of pre-implant lower urinary tract symptoms on postoperative urinary morbidity after permanent prostate brachytherapy. (2012)
  • Author(s): Teishima J, Iwamoto H, Miyamoto K, Shoji K, Masumoto H, Inoue S, Kobayashi K, Kajiwara M, Matsubara A.
  • Journal Title: Int J Urol. Volume: 19(12) Issue: 12 Pages: 1083-1089
  • DOI: 10.1111/j.1442-2042.2012.03105.x
  • NAID: 10031136952
  • Peer Reviewed
• [Journal Article] Impact of laparoscopic experience on the proficiency gain of urologic surgeons in robot-assisted surgery. (2012)
  • Author(s): Teishima J, Hattori M, Inoue S, Ikeda K, Hieda K, Miyamoto K, Shoji K, Hayashi T, Kobayashi K, Kajiwara M, Egi H, Ohdan H, Matsubara A..
  • Journal Title: J Endourol. Volume: 26(12) Issue: 12 Pages: 1635-1638
  • DOI: 10.1089/end.2012.0308
  • Peer Reviewed
• [Journal Article] Identification of lymphatic pathway involved in the spread of bladder cancer: Evidence obtained from fluorescence navigation with intraoperatively injected indocyanine green. (2012)
  • Author(s): Inoue S, Shiina H, Mitsui Y, Yasumoto H, Matsubara A, Igawa M.
  • Journal Title: Can Urol Assoc J. Volume: Sep 10 Pages: 1-7
  • DOI: 10.5489/cuaj.12096
  • Peer Reviewed
• [Presentation] 去勢抵抗性前立腺癌におけるregIVの意義 (2014)
  • Author(s): 亭島 淳、正路晃一、山中亮憲、後藤景介、永松弘孝、大原慎也、林 哲太郎、岡 清貴、大上直秀、安井 弥、松原昭郎
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2014-09-25
• [Presentation] Endocrine FGFs promote progression in prostate cancer. (2014)
  • Author(s): 永松弘孝、亭島 淳、後藤景介、松原昭郎
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2014-09-25
• [Presentation] Regenerating islet-derived family, member 4 enhances cell proliferation, castration-resistance, and chemoresistance through acceleration of neuroendocrine differentiation in human prostate cancer cells. (2014)
  • Author(s): Jun Teishima, Koichi Shoji, Keisuke Goto, Ryoken Yamaoka, Hiroyuki Shikuma, Hirotaka Nagamatsu, Kiyotaka Oka, Shunsuke Shinmei, Hiroyuki Kitano, Shinya Ohara, Tetsutaro Hayashi, Norihide Oue, Wataru Yasui, Akio Matsubara.
  • Organizer: AUA Annual Meeting 2014
  • Place of Presentation: Orlando
  • Year and Date: 2014-05-19
• [Presentation] Restoration of Fibroblast Growth Factor Receptor 2IIIb Enhances the Chemosensitivity of Human Prostate Cancer Cells. (2014)
  • Author(s): Koichi Shoji, Jun Teishima, Shinya Ohara, Akio Matsubara
  • Organizer: AUA Annual Meeting 2014
  • Place of Presentation: Orlando
  • Year and Date: 2014-05-16 – 2014-05-21
• [Presentation] FGF19 subfamily promotes progression in prostate cancer cells. (2014)
  • Author(s): Hirotaka Nagamatsu, Jun Teishima, Keisuke Goto, Akio Matsubara
  • Organizer: AUA Annual Meeting 2014
  • Place of Presentation: Orlando
  • Year and Date: 2014-05-16 – 2014-05-21
• [Presentation] 前立線癌におけるReg Ⅳの意義. (2014)
  • Author(s): 亭島 淳、永松弘孝、北野弘之、後藤景介、岡 清貴、正路晃一、大上直秀、安井 弥、松原昭郎
  • Organizer: 第102回日本泌尿器科学会総会
  • Place of Presentation: 神戸市
  • Year and Date: 2014-04-27
• [Presentation] FGF19は前立線癌細胞の増殖を促進する. (2014)
  • Author(s): 永松弘孝、亭島 淳、後藤景介、正路晃一、松原昭郎
  • Organizer: 第102回日本泌尿器科学会総会
  • Place of Presentation: 神戸市
  • Year and Date: 2014-04-25
• [Presentation] 前立腺癌細胞におけるFGFR2Ⅲbの発現回復は化学療法感受性を増強する. (2014)
  • Author(s): 正路晃一、亭島 淳、大原慎也、松原昭郎
  • Organizer: 第102回日本泌尿器科学会総会
  • Place of Presentation: 神戸市
  • Year and Date: 2014-04-25
• [Presentation] Regenetrating islet-derived family, member 4 enhances cell proliferation, castration-resistance and chemoresistance through aceleration of neuroendocrine differentiation in human prostate cancer cells. (2014)
  • Author(s): J.Teishima, K.Shoji, K.Goto, R.Yamanaka, H.Shikuma, H.Nagamatsu, S.Shinmei, H.Kitano, S.Ohara, T.hayashi, N.Oue, W.Yasui, A.Matsubara
  • Organizer: 29th Annual EAU Congress
  • Place of Presentation: Stockholm
  • Year and Date: 2014-04-12
• [Presentation] FGF19 subfamily promotes orogression in prostate cancer. (2014)
  • Author(s): Nagamatsu H, Teishima J, Goto K, Matsubara A
  • Organizer: The 4th Congress of Asian Pacific Prostate Society (APPS2014)
  • Place of Presentation: 沖縄
  • Invited
• [Presentation] FGF19は前立腺癌細胞の増殖を促進する (2013)
  • Author(s): 永松弘孝、亭島 淳、正路晃一、後藤景介、岡清貴、神明俊輔、松原昭郎
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜市
• [Presentation] 前立腺癌細胞におけるFGF9の集積は上皮間葉移行およびVEGFAの誘導に関与する (2013)
  • Author(s): 亭島 淳、林哲太郎、正路晃一、岡清貴、永松弘孝、後藤景介、神明俊輔、松原昭郎
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜市
• [Presentation] CAST 法により見いだされたPRL1の前立腺癌における発現と機能解析 (2013)
  • Author(s): 神明俊輔、林哲太郎、坂 直也、後藤景介、仙谷和弘、亭島 淳、松原昭郎、大上直秀、國 弘基、安井 弥
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜市
• [Presentation] The search for secreted protein in prostate cancer by the Escherichia coli ampicillin secretion trap: expression of NBL1 is highly restricted in prostate and related in progression. (2012)
  • Author(s): Hayashi T, Ohara S, Teishima J, Sentani K, Oue N, Sakamoto N, Yasui W, Matsubara A
  • Organizer: 107th Annual Meeting of the American Urological Association.
  • Place of Presentation: Atlanta, USA
• [Presentation] A Role of FGFR2IIIb in the Chemosensitivity of Castration-resistant Prostate Cancer Cells. (2012)
  • Author(s): Shoji K, Teishima J, Ohara S, Hayashi T, Matsubara A.
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] The Effects of Fibroblast Growth Factor 9 in Prostate Cancer Cells. (2012)
  • Author(s): Teishima J, Shoji K, Hayashi T, Oka K, Nagamatsu H, Ohara S, Matsubara A.
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] Expression of TMEM50B is associated with tumor progression in prostate cancer. (2012)
  • Author(s): Hayashi T, Sentani K, Anami K, Nitou Y, Shinmei S, Goto K, Sakamoto N, Oue N, Matsubara A, Yasui W
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] MicroRNA-155 is a predictive marker for survival in patients with renal cell carcinoma. (2012)
  • Author(s): Shinmei S, Sakamoto N, Goto K, Sentani K, Anami K, Hayashi T, Teishima J, Matsubara A, Oue N, Yasui W.
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] Overexpression of miR-486 is a potential prognostic factor after nephrectomy in advanced renal cell carcinoma. (2012)
  • Author(s): Goto K, Shinmei S, Sakamoto N, Sentani K, Anami K, Hayashi T, Teishima J, Matsubara A, Oue N, Yasui W
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] 前立腺癌の化学療法感受性におけるFGFR2の役割 (2012)
  • Author(s): 正路晃一、亭島 淳、林哲太郎、柴田昌紀、池田健一郎、稗田圭介、宮本克利、増本弘史、井上省吾、小林加直、梶原充、松原昭郎
  • Organizer: 第100回日本泌尿器科学会総会
  • Place of Presentation: 横浜
• [Presentation] 前立腺癌細胞の増殖と浸潤におけるFGF9の役割 (2012)
  • Author(s): 亭島淳、林哲太郎、正路晃一、大原慎也、松原昭郎
  • Organizer: 第100回日本泌尿器科学会総会
  • Place of Presentation: 横浜