Isolation of prostate cancer stem-like cell and establishment of specific prostate cancer immunotherapy

• Project/Area Number: 24592399
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Sapporo Medical University
• Principal Investigator: MASUMORI Naoya 札幌医科大学, 医学部, 教授 (20295356)
• Co-Investigator(Kenkyū-buntansha): KITAMURA Hiroshi 札幌医科大学, 医学部, 講師 (00404674) ; NISHIDA Sachiyo 札幌医科大学, 医学部, 研究員 (10509566)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 前立腺癌 / がん幹細胞 / 癌幹細胞 / 幹細胞

Outline of Final Research Achievements

The purpose of this study was to investigate prostate Cancer Stem Cells/ Cancer Initiating Cell (CSCs/CICs). Prostate CSCs ／ CICs were isolated as ALDH1 high cells using the ALDH1 activity assay. We found that prostate CSCs ／ CICs expressed higher levels of growth factors including hepatocyte growth factor (HGF). The results indicate that HGF secreted by prostate CSCs ／ CICs. The purpose of the next study was to evaluate the relationship between expression of HGF in prostate tissues and biochemical recurrence after radical prostatectomy. Immunohistochemical staining of HGF was compared to biochemical recurrence after radical prostatectomy. Patients with tumors exhibiting HGF positivity of 5% or more had a significantly shorter biochemical recurrence-free period than that of patients whose tumor HGF positivity was less than 5%. Our data suggests that therapeutic targeting of CSCs/CICs in prostate cancer is a future possibility

Research Products

• [Journal Article] Expression of Hepatocyte Growth Factor in Prostate Cancer May Indicate a Biochemical Recurrence After Radical Prostatectomy (2015)
  • Author(s): SACHIYO NISHIDA,YOSHIHIKO HIROHASHI, TOSHIHIKO TORIGOE, MASANORI NOJIMA, RYUTA INOUE, HIROSHI KITAMURA, TOSHIAKI TANAKA, HIROKO ASANUMA, NORIYUKI SATO, NAOYA MASUMORI
  • Journal Title: Anticancer Research Volume: 35 Pages: 413-418
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Prostate cancer stem-like cells/cancer-initiating cells have an autocrine system of hepatocyte growth factor. (2013)
  • Author(s): Nishida S, Hirohashi Y, Torigoe T, Inoue R, Kitamura H, Tanaka T, Takahashi A, Asanuma H, Masumori N, Tsukamoto T, Sato N.
  • Journal Title: Cancer Sci Volume: 104 Issue: 4 Pages: 431-6
  • DOI: 10.1111/cas.12104
  • Peer Reviewed
• [Journal Article] Gene expression profiles of prostate cancer stem cells isolated by aldehyde dehydrogenase activity assay. (2012)
  • Author(s): Nishida S, Hirohashi Y, Torigoe T, Kitamura H, Takahashi A, Masumori N, Tsukamoto T, Sato N.
  • Journal Title: Journal of Urology Volume: 188 Issue: 1 Pages: 294-299
  • DOI: 10.1016/j.juro.2012.02.2555
  • Peer Reviewed
• [Presentation] Expression of Hepatocyte Growth Factor in Prostate Cancer May Indicate a Biochemical Recurrence After Radical Prostatectomy (2015)
  • Author(s): SACHIYO NISHIDA,YOSHIHIKO HIROHASHI, TOSHIHIKO TORIGOE, MASANORI NOJIMA, RYUTA INOUE, HIROSHI KITAMURA, TOSHIAKI TANAKA, HIROKO ASANUMA, NORIYUKI SATO, NAOYA MASUMORI
  • Organizer: 第30回ヨーロッパ泌尿器科学会総会
  • Place of Presentation: マドリッド（スペイン）
  • Year and Date: 2015-03-20 – 2015-03-24
• [Presentation] Prostate cancer stem-like cells/cancer-initiating cells have an autocrine system of hepatocyte growth factor. (2013)
  • Author(s): Sachiyo Nishida, Yoshihiko Hirohashi, Toshihiko Torigoe, Toshiaki Tanaka, Ryuta Inoue, Akari Takahashi, Hiroshi Kitamura, Naoya Masumori, Noriyuki Sato and Taiji Tsukamoto
  • Organizer: 2013年アメリカ泌尿器科学会総会
  • Place of Presentation: サンディエゴ
• [Presentation] 前立腺がん幹細胞におけるhepatocyte-growth factor (HGF)の自己分泌機構 (2013)
  • Author(s): 西田幸代,廣橋良彦,鳥越俊彦,井上隆太, 田中俊明,北村寛,高橋あかり ,浅沼広子, 舛森直哉,佐藤昇志
  • Organizer: 第102回日本病理学会総会
  • Place of Presentation: 札幌
• [Presentation] 前立腺癌のhepatocyte growth factor発現は前立腺全摘後PSA再発の予測因子となりうる (2013)
  • Author(s): 西田幸代、廣橋良彦、鳥越俊彦、野島正寛、北村寛、佐藤昇志、舛森直哉
  • Organizer: 第29回前立腺シンポジウム
  • Place of Presentation: 東京
• [Presentation] ALDH1活性分析により分離された前立腺癌幹細胞集団の特徴. (2012)
  • Author(s): 西田幸代, 鳥越俊彦, 廣橋良彦, 北村 寛, 舛森直哉,佐藤昇志, 塚本泰司.
  • Organizer: 第100回日本泌尿器科学会総会
  • Place of Presentation: 横浜
• [Presentation] 前立腺癌幹細胞におけるHGF autocrineシステム (2012)
  • Author(s): 西田幸代, 廣橋良彦, 鳥越俊彦, 田中俊明, 北村 寛, 高橋あかり, 舛森直哉,塚本泰司, 佐藤昇志.
  • Organizer: 第71回日本癌学会
  • Place of Presentation: 札幌
• [Presentation] ALDH1活性分離した前立腺癌幹細胞の特徴と成長因子の自己分泌機構. (2012)
  • Author(s): 西田幸代, 廣橋良彦, 鳥越俊彦, 田中俊明, 北村 寛, 舛森直哉, 佐藤昇志, 塚本泰司.
  • Organizer: 第387回北海道地方会
  • Place of Presentation: 札幌