| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The urinary bladder is one of the organs most often affected by the side effects of pelvic radiotherapy. In this study, we produced bone marrow-derived cell sheets in temperature-responsive culture dishes that release the monolayer sheets intact. We then determined if the recovered cell sheets could restore function to irradiated urinary bladders. After 4 weeks, the cell sheet-transplanted bladders had smooth muscle layers and nerve fibers in proportions that were significantly larger than those of the control bladders. In cystometric investigations, cell-free sheet control rats had frequent and irregular periods of micturition; however, the cell sheet-transplanted rats voided at regular intervals of micturition, which is typical for rats with uninjured bladders. There were 24 growth factors to promote reconstruction of urinary bladders in the cell sheet-transplanted urinary bladders. In conclusion, cell sheet engineering has great potential to restore damaged urinary bladders.
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