Bone marrow-derived cell sheets restore structure and function of radiation-injured urinary bladders
| Project/Area Number |
24592427
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 膀胱再生 / 排尿障害 / 放射線照射傷害 / 骨髄細胞 / 温度応答性培養皿 / 細胞シート / ラット / Tissue Engineering / 下部尿路再生 / 温度感受性培養皿 / 再生医療研究 / 骨髄由来細胞 / 放射線照射 / 膀胱 |
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| Outline of Final Research Achievements |
The urinary bladder is one of the organs most often affected by the side effects of pelvic radiotherapy. In this study, we produced bone marrow-derived cell sheets in temperature-responsive culture dishes that release the monolayer sheets intact. We then determined if the recovered cell sheets could restore function to irradiated urinary bladders. After 4 weeks, the cell sheet-transplanted bladders had smooth muscle layers and nerve fibers in proportions that were significantly larger than those of the control bladders. In cystometric investigations, cell-free sheet control rats had frequent and irregular periods of micturition; however, the cell sheet-transplanted rats voided at regular intervals of micturition, which is typical for rats with uninjured bladders. There were 24 growth factors to promote reconstruction of urinary bladders in the cell sheet-transplanted urinary bladders. In conclusion, cell sheet engineering has great potential to restore damaged urinary bladders.
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Report
(4 results)
Research Products
(37 results)
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[Journal Article] Implantation of adipose-derived cells reconstructs functional urethral sphincters in rabbit cryo-injured urethra2014
Author(s)
Gautam S S, Imamura T, Ishizuka O, Zhang L, Yamagishi T, Yokoyama H, Minagawa T, Ogawa T, Kurizaki Y, Kato H, Nishizawa O
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Journal Title
Tissue Engineering Part A
Volume: 20
Issue: 13-14
Pages: 1971-1979
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] ヒト膀胱におけるセロトニン受容体発現の検討2014
Author(s)
今村哲也, 石塚 修, 小川輝之, 横山 仁, 平形志朗, 永井 崇, 皆川倫範, 中沢昌樹, 西澤 理
Organizer
第21回日本排尿機能学会
Place of Presentation
岡山コンベンションセンター（岡山県 岡山市）
Year and Date
2014-09-17 – 2014-09-20
Related Report
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[Presentation] 骨髄由来幹細胞による膀胱再生医療研究2014
Author(s)
今村哲也, 石塚 修, 西澤 理
Organizer
第102回日本泌尿器科学会総会
Place of Presentation
神戸国際会議場（兵庫県 神戸市）
Year and Date
2014-04-24 – 2014-04-27
Related Report
Invited
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