Functional urotherial-sheet reconstruction from transformed epithelial cells using hollow-fiber cell-culture system

• Project/Area Number: 24592435
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Nagoya City University
• Principal Investigator: MARUYAMA Tetsuji 名古屋市立大学, 医学(系)研究科(研究院), 研究員 (50305546)
• Co-Investigator(Kenkyū-buntansha): MIZUNO Kentaro 名古屋市立大学, 大学院医学研究科, 講師 (70448710) ; HAYASHI Yutaro 名古屋市立大学, 大学院医学研究科, 准教授 (40238134) ; KOHRI Kenjiro 名古屋市立大学, その他部局, 学長 (30122047) ; KOJIMA Yosiyuki 福島県立医科大学, 医学部, 教授 (60305539)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: KIT陽性細胞 / シェアーストレス / 伸展刺激 / ホローファイバー / 上皮間葉系誘導 / 平滑筋細胞 / 尿路上皮細胞 / 再生医療 / 細胞工学 / 移行上皮細胞

Outline of Final Research Achievements

With cytoengineering method, we have made urotherial sheet with functional smooth muscle layer showing regular special pattern. These cells were under laminar flow stimulation exerted shear and stretch stress, and/or electrical stimulation using hollow-fiber cell-culture system. In addition, ES cells were used to induce these urotherial and smooth muscle cell to minimize preparing time and effort.

Research Products

• [Journal Article] Can spongioplasty prevent fistula formation and correct penile curvature in TIP urethroplasty for hypospadias? (2013)
  • Author(s): Hayashi Y, Mizuno K, Moritoki Y, Nakane A, Kato T, Kurokawa S, Kamisawa H, Nishio H, Kohri K, Kojima Y.
  • Journal Title: UROLOGY Volume: 81 Issue: 6 Pages: 1330-1335
  • DOI: 10.1016/j.urology.2013.01.005
  • Peer Reviewed
• [Journal Article] Circumcision related to urinary tract infections, sexually transmitted infections, human immunodeficiency virus infections, and penile and cervical cancer. (2013)
  • Author(s): Hayashi Y, Kohri K.
  • Journal Title: International Journal of Urology Volume: 20 Issue: 8 Pages: 769-775
  • DOI: 10.1111/iju.12154
  • NAID: 10031190534
  • Peer Reviewed
• [Journal Article] Gene expression profile during testicular development in patients with SRY-negative 46, XX testicular disorder of sex development (2013)
  • Author(s): Mizuno K, Kojima Y, Kamisawa H, Moritoki Y, Nishio H, Kohri K, Hayashi Y
  • Journal Title: UROLOGY Volume: 82: 1453 Issue: 6 Pages: 1453-1453
  • DOI: 10.1016/j.urology.2013.08.040
  • Peer Reviewed
• [Journal Article] Attenuation of spermatogonial stem cell activity in cryptorchid testes. (2012)
  • Author(s): Kamisawa H, Kojima Y, Mizuno K, Imura M, Hayashi Y, Kohri K.
  • Journal Title: The Journal of Urology Volume: 187 Issue: 3 Pages: 1047-1052
  • DOI: 10.1016/j.juro.2011.10.170
  • Peer Reviewed
• [Journal Article] Intratesticular pressure after testicular torsion as a predictor of subsequent spermatogenesis : a rat model (2012)
  • Author(s): Moritoki Y, Kojima Y, Mizuno K, Kamisawa H, Kohri K, Hayashi Y
  • Journal Title: BJU Int Volume: 109 Issue: 3 Pages: 466-70
  • DOI: 10.1111/j.1464-410x.2011.10279.x
  • Peer Reviewed
• [Presentation] Distinctive changes in histone H3K4 modification mediated via Kdm5a expression in spermatogonial stem cells of cryptorchid testes. (2014)
  • Author(s): Nishio Hidenori, Hayashi Yutaro, Moritoki Yoshinobu, Kamisawa Hideyuki, Kurokawa Satoshi, Nakane Akihiro, Mizuno Kentaro, Maruyama Tetsuji, Kojima Yoshiyuki, Kohri Kenjiro
  • Organizer: American Urological Association Annual Meeting 2014
  • Place of Presentation: Orlando(USA)
  • Year and Date: 2014-05-16 – 2014-05-21
• [Presentation] Testosterone treatment prior to hypospadias surgery transiently upregulates VEGFA mediated via AR and causes. (2014)
  • Author(s): Nishio Hidenori, Hayashi Yutaro, Moritoki Yoshinobu, Kamisawa Hideyuki, Kurokawa Satoshi, Nakane Akihiro, Mizuno Kentaro, Maruyama Tetsuji, Kojima Yoshiyuki, Kohri Kenjiro
  • Organizer: American Urological Association Annual Meeting 2014
  • Place of Presentation: Orlando(USA)
  • Year and Date: 2014-05-16 – 2014-05-21
• [Presentation] Elucidation of genomic DNA structures distinctive of patients with 46,XX testicular DSD using genome-wide analyses (2013)
  • Author(s): Mizuno Kentaro, Kojima Yoshiyuki, Kurokawa Satoshi, Kamisawa Hideyuki, Umemoto Yukihiro, Moritoki Yoshinobu, Nishio Hidenori, Sasaki Shoichi, Hayashi Yutaro, Kohri Kenjiro
  • Organizer: 10th International Congress of Andrology
  • Place of Presentation: Melbourne,Australia
• [Presentation] Preoperative testicular position and volume in Japanese children with unilateral cryptorchidism (2013)
  • Author(s): Kamisawa Hideyuki, Mizuno Kentaro, Kurokawa Satoshi, Imura Makoto, Moritoki Yoshinobu, Nishio Hidenori, Nakane Akihiro, Kato Toshiki, Maruyama Tetsuji, Hayashi Yutaro, Kohri Kenjiro
  • Organizer: The Society for Pediatric Urology 61th Annual Meeting
  • Place of Presentation: San Diego Convention Center( San Diego, USA)
• [Presentation] The role of miR-135a via regulation of Foxo1 gene expression in maintenance of spermatogonial stem cells (2013)
  • Author(s): Moritoki Yoshinobu, Kojima Yoshiyuki, Mizuno Kentaro, Shibata Yasuhiro, Kamisawa Hideyuki, Imura Makoto, Kurokawa Satoshi, Nakane Akihiro, Kato Toshiki, Maruyama Tetsuji, Hayashi Yutaro, Kohri Kenjiro
  • Organizer: San Diego(USA)
  • Place of Presentation: San Diego Convention Center( San Diego, USA)
• [Presentation] Surgical repair for hypospadias with penile curvature (2013)
  • Author(s): Hayashi Yutaro, Mizuno Kentaro, Kurokawa Satoshi, Kohri Kenjiro
  • Organizer: 15th Annual Congress of Asia-Pacific Association of Pediatric Urologists
  • Place of Presentation: Taipei Tzuchi Hospital,The Buddhist Tzuchi Medical Foundatior(Taipei,Taiwan)
• [Presentation] Functional analysis of spermatogonial stem cells in cryptorchidism using EEF1A1 and TPT1 gene expression as indexes of cell (2012)
  • Author(s): Mizuno Kentaro, Kojima Yoshiyuki, Kamisawa Hideyuki, Kurokawa Satoshi,
  • Organizer: American Urological Association Annual Meeting 2012
  • Place of Presentation: Atlanta(USA)
• [Presentation] Adenovirus-mediated Dax1 gene transfer enhances the function of Sertoli cells via the SCF/C-KIT pathway. (2012)
  • Author(s): Kurokawa Satoshi, Kojima Yoshiyuki, Mizuno Kentaro, Nakane Akihiro,
  • Organizer: American Urological Association Annual Meeting 2012
  • Place of Presentation: Atlanta(USA)