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The investigation of the mechanism about the kidney generation using embryonic stem cells with inducible gene expression by assesment of FACS, and the application to kidney regeneration medicine

Research Project

Project/Area Number 24592436
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Urology
Research InstitutionNagoya City University

Principal Investigator

NAKANE Akihiro  名古屋市立大学, 医学(系)研究科(研究院), 研究員 (70464568)

Co-Investigator(Kenkyū-buntansha) MARUYAMA Tetsuji  名古屋市立大学, 大学院医学研究科, 研究員 (50305546)
MIZUNO Kentaro  名古屋市立大学, 大学院医学研究科, 講師 (70448710)
HAYASHI Yutaro  名古屋市立大学, 大学院医学研究科, 准教授 (40238134)
KOHRI Kenjiro  名古屋市立大学, その他部局等, 学長 (30122047)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords再生医療 / 胚性幹細胞 / FACS / 遺伝子導入 / ES細胞 / 腎不全
Outline of Final Research Achievements

Transcription factor Pax2 is essential for kidney development. We have generated embryonic stem (ES) cell lines that repress Pax2 expression and examined their differentiation potential by embryoid body (EB) formation. EBs were cultured with or without retinoic acid and activin A. EBs were analyzed by reverse transcription (RT)-PCR and immunocytochemical analysis. Aquaporin-1 (Aqp1) and integrin 8 were upregulated when cells were induced to form EBs. With retinoic acid and activin A, EBs overexpressed Pax2-induced Pax8, BMP4, BMP7, and Podocin. ES cells that expressed aquaporin-1 and Pax2 incresed by analysis of the FACS cell sorting. ES cell lines with inducible Pax2 expression may be useful for dissecting genetic cascades functioning in the development of various organs, including the kidney.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (2 results)

All 2014 Other

All Presentation (2 results)

  • [Presentation] 始原生殖細胞発生に必須な新規遺伝子Dullardの機能解析 ―ノックアウトマウスでの検討―2014

    • Author(s)
      中根 明宏
    • Organizer
      第23回日本小児泌尿器科学会総会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-07-09 – 2014-07-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] Pax2遺伝子導入ES細胞における分化のメカニズム解析と腎構成細胞形成の検討

    • Author(s)
      中根 明宏、水野 健太郎、丸山 哲史、林 祐太郎、郡 健二郎
    • Organizer
      第63回日本泌尿器科学会中部総会
    • Place of Presentation
      愛知県産業労働センター(名古屋)
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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