Functional analysis of urinary exosome in ADPKD

• Project/Area Number: 24592439
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Juntendo University (2013-2014); Teikyo University (2012)
• Principal Investigator: HORIE Shigeo 順天堂大学, 医学(系)研究科(研究院), 教授 (40190243)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: ADPKD / 多発性嚢胞腎 / exosome / microRNA / 尿中microRNA / microvesicle / LC/MS法 / 尿中バイオマーカー / 奇形学

Outline of Final Research Achievements

Urinary exosome was isolated from either CKD Gstage4 or ADPKD patients upon consent, and the identified microRNA was compared between these 2 groups. MicroRNAs specific for Wnt/b-catenin/GSK-3, regulating Oncogenes, associated with renal cell carcinoma, tumor suppressor gene, and inhibitory for inflammation were more expressed in ADPKD.
Specific microRNAs identified from urinary exosome of ADPKD patients can be either prognostic or predictive for the clinical course of ADPKD.

Research Products

• [Journal Article] executive summary from a Kidney Disease: Improving Global Outcomes (KDIGO) Controversies Conference. (2015)
  • Author(s): Chapman AB, Devuyst O, Eckardt KU, Gansevoort RT, Harris T, Horie S, Kasiske BL, Odland D, Pei Y, Perrone RD, Pirson Y, Schrier RW, Torra R, Torres VE, Watnick T, Wheeler DC
  • Journal Title: Kidney Int Volume: Epub ahead of print Issue: 1 Pages: 17-27
  • DOI: 10.1038/ki.2015.59
  • Peer Reviewed
• [Journal Article] New strategy for the treatment of autosomal dominant polycystic kidney disease (2015)
  • Author(s): Shigeo Horie
  • Journal Title: Nihon Jinzo Gakkai Shi Volume: 57 Pages: 254-61
  • NAID: 40020351566
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Association of arginine vasopressin surrogate marker urinary copeptin with severity of autosomal dominant polycystic kidney disease (ADPKD). (2015)
  • Author(s): Nakajima A, Lu Y, Kawano H, Horie S, Muto S.
  • Journal Title: Clin Exp Nephrol. Volume: Epub ahead of print
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The effect of tolvaptan on autosomal dominant polycystic kidney disease patients: a subgroup analysis of the Japanese patient subset from TEMPO 3:4 trial. (2015)
  • Author(s): Muto S, Kawano H, Higashihara E, Narita I, Ubara Y, Matsuzaki T, Ouyang J, Torres VE, Horie S.
  • Journal Title: Clin Exp Nephrol. Volume: Epub ahead of print
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Exploring urinary biomarkers in autosomal dominant polycystic kidney disease. (2014)
  • Author(s): Kawano H, Muto S, Ohmoto Y, Iwata F, Fujiki H, Mori T, Yan L, Horie S.
  • Journal Title: Clin Exp Nephrol. Volume: Epub ahead of print Issue: 5 Pages: 968-973
  • DOI: 10.1007/s10157-014-1078-7
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The cutting-edge of medicine; the pathology and new treatment of ADPKD (2014)
  • Author(s): Muto S, Horie S.
  • Journal Title: Nihon Naika Gakkai Zasshi Volume: 103 Pages: 978-82
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 多発性嚢胞腎の病態と治療：新たな展開 (2014)
  • Author(s): 武藤 智．堀江重郎
  • Journal Title: 日内会誌 Volume: 103(46) Pages: 978-82
  • NAID: 130005061047
• [Journal Article] I巻頭トピックス 8 常染色体優性多発性嚢胞腎（ADPKD）の病態と治療の新しい展開 (2014)
  • Author(s): 武藤 智．堀江重郎
  • Journal Title: 腎疾患・透析 Volume: - Pages: 41-4
• [Journal Article] Telmisartan ameliorates fibrocystic liver disease in an orthologous rat model of human autosomal recessive polycystic kidney disease (2013)
  • Author(s): Yoshihara D, Kugita M, Sasaki M, Horie S, Nakanishi K, Abe T, Aukema HM, Yamaguchi T, Nagao S
  • Journal Title: PLoS One Volume: 巻８巻
  • Peer Reviewed
• [Journal Article] 透析患者の検査値の読み方 (2013)
  • Author(s): 武藤 智
  • Journal Title: 画像診断12ACDK Volume: 第7章
• [Journal Article] 多発性嚢胞腎．腎疾患に対する薬剤の使用方法 (2013)
  • Author(s): 武藤 智
  • Journal Title: 腎疾患治療マニュアル Volume: 74 Pages: 272-4
• [Journal Article] I 症例と病理カンファレンス編 A 腎癌CPC 症例1 嚢胞に伴う腎癌 (2013)
  • Author(s): 武藤 智，堀江重郎
  • Journal Title: 実践的 泌尿器腫瘍病理診断 Volume: - Pages: 2-6
• [Journal Article] 多発性嚢胞腎治療 (2013)
  • Author(s): 武藤 智，堀江重郎
  • Journal Title: 今日の治療と看護 Volume: - Pages: 627-8
• [Journal Article] V2受容体拮抗薬による多発性嚢胞腎治療 (2013)
  • Author(s): 武藤 智，堀江重郎
  • Journal Title: Annual Review腎臓 Volume: - Pages: 266-73
• [Journal Article] 原因疾患の管理 多発性嚢胞腎 (2013)
  • Author(s): 武藤 智，堀江重郎
  • Journal Title: Medical View Point Volume: -
• [Journal Article] 血尿（特発性腎出血を含む） (2013)
  • Author(s): 武藤 智
  • Journal Title: 今日に治療指針 私はこう治療している Volume: -
• [Journal Article] Interferon-α Treatment for Growing Teratoma Syndrome of the Testis. (2013)
  • Author(s): Inoue M, Hisasue S, Nagae M, China T, Saito K, Isotani S, Yamaguchi R, Ide H, Muto S, Horie S.
  • Journal Title: Case Rep Nephrol Urol. Volume: 3 Pages: 40-45
  • Peer Reviewed
• [Journal Article] Maintenance therapy with intravesical bacillus Calmette-Guerin in patients with intermediate- or high-risk non-muscle-invasive bladder cancer. (2013)
  • Author(s): Muto S, Nakajima A, Horiuchi A, Inoue M, China T, Saito K, Isotani S, Hisasue S, Yamaguchi R, Ide H, Horie S.
  • Journal Title: Jpn J Clin Oncol Volume: 43 Pages: 305-313
  • Peer Reviewed
• [Journal Article] Chemoprevention of prostate cancer: soy isoflavones and curcumin. (2012)
  • Author(s): Horie S
  • Journal Title: Korean J Urol Volume: 53 Pages: 665-672
  • Peer Reviewed
• [Journal Article] The relationship between second-to-fourth digit ratio and female gender identity. (2012)
  • Author(s): Hisasue S, Sasaki S, Tsukamoto T, Horie S.
  • Journal Title: J Sex Med Volume: 9 Pages: 2903-2910
  • Peer Reviewed
• [Journal Article] Renal disease progression in autosomal dominant polycystic kidney disease. (2012)
  • Author(s): Higashihara E, Horie S, Muto S, Mochizuki T, Nishio S, Nutahara K.
  • Journal Title: Clin Exp Nephrol. Volume: 16 Pages: 622-628
  • NAID: 10031058310
  • Peer Reviewed
• [Journal Article] Cure of ADPKD by selection for spontaneous genetic repair events in Pkd1-mutated iPS cells. (2012)
  • Author(s): Cheng LT, Nagata S, Hirano K, Yamaguchi S, Horie S, Ainscough J, Tada T.
  • Journal Title: PLoS One Volume: 7
  • NAID: 120003796491
  • Peer Reviewed
• [Journal Article] Testosterone promotes DNA damage response under oxidative stress in prostate cancer cell lines. (2012)
  • Author(s): Ide H, Lu Y, Yu J, China T, Kumamoto T, Koseki T, Yamaguchi R, Muto S, Horie S.
  • Journal Title: Prostate Volume: 72 Pages: 1407-1411
  • Peer Reviewed
• [Journal Article] 特集：多発性嚢胞腎―最新の知見と今後の課題 ADPKDにおける腎容積と腎機能 (2012)
  • Author(s): 堀江重郎，武藤 智
  • Journal Title: 日腎会誌 Volume: 54 Pages: 501-5
• [Presentation] Novel approach to estimate kidney volumes using computer-assisted segmentation tools in ADPKD. (2014)
  • Author(s): Muto S, Isotani S, Shimoyama H, Kawano H, Masumoto J, Kotera K, Kitamura K, Kimura M, Saito K, Ide H, Yamaguchi R, Horie S.
  • Organizer: 47th Annual Meeting of the American Society of Nephrology
  • Place of Presentation: Philadelphia
  • Year and Date: 2014-11-12 – 2014-11-16
• [Presentation] ADPKD患者の尿中copeptin． (2014)
  • Author(s): 武藤 智，河野春奈，野口尊弘，杉浦正一郎，古謝将之，堀内 明，下山博史，北村香介，常盤紫野，木村将貴，斎藤恵介，磯谷周治，井手久満，山口雷蔵，堀江重郎．
  • Organizer: 第44回日本腎臓学会東部学術大会
  • Place of Presentation: 東京
  • Year and Date: 2014-10-24 – 2014-10-25
• [Presentation] 多発性嚢胞腎患者全国登録による多施設共同研究（J-PKDレジストリー研究）；中間報告． (2014)
  • Author(s): 武藤 智，望月俊雄，土谷 健，西尾妙織，花岡一成，鶴屋和彦，石村栄治，成田一衛，乳原善文，奴田原紀久雄，堀江重郎．
  • Organizer: 第57回日本腎臓学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-07-04 – 2014-07-06
• [Presentation] 腎三次元立体モデルを用いて選択的開窓術を行ったADPKD嚢胞感染患者の一例． (2014)
  • Author(s): 堀内 明，武藤 智，中島晶子，杉浦正一郎，古謝将之，下山博史，北村香介，常盤紫野，斉藤恵介，磯谷周治，土井直人，井手久満，山口雷蔵，堀江重郎．
  • Organizer: 第615回日本泌尿器科学会東京地方会
  • Place of Presentation: 東京
  • Year and Date: 2014-06-21
• [Presentation] The relationship between liver cyst volume and QOL in ADPKD. (2014)
  • Author(s): Muto S, Ando M, Nishio S, Narita I, Kamura K, Tsuchiya K, Mochizuki T, Tsuruya K, Ubara Y, Nutahara K, Horie S.
  • Organizer: The 14th Asian Pacific Congress of Nephrology
  • Place of Presentation: Tokyo
  • Year and Date: 2014-05-14 – 2014-05-17
• [Presentation] The compiling data at the time of enrollment in J-PKD registry. (2014)
  • Author(s): Muto S, Mochizuki T, Tsuchiya K, Nishio S, Hanaoka K, Tsuruya K, Ishimura E, Kamura K, Narita I, Nutahara K, Horie S.
  • Organizer: The 14th Asian Pacific Congress of Nephrology
  • Place of Presentation: Tokyo
  • Year and Date: 2014-05-14 – 2014-05-17
• [Presentation] The compiling data at the time of enrollment in J-PKD registry. (2013)
  • Author(s): Horie S, Muto S, Mochizuki T, Tsuchiya T, Nishio S, Hanaoka K, Tsuruya K, Ishimura E, Narita I, Kamura K, Ubara Y, Nutahara K
  • Organizer: World Congress of Nephrology 2013
  • Place of Presentation: Hong Kong, China
• [Presentation] The compiling data at the time of enrollment in J-PKD registry (2013)
  • Author(s): Muto S, Mochizuki T, Tsuchiya K, Nishio S, Hanaoka K, Tsuruya K, Ishimura E, Narita I, Kamura K, Ubara Y, Ando M, Nutahara K, Horie S
  • Organizer: 46th Annual Meeting of the American Society of Nephrology
  • Place of Presentation: Atlanta, USA
• [Presentation] 反復する嚢胞感染に対して術前検討用腎三次元立体モデルを作成し開窓術を行ったADPKD患者の一例 (2013)
  • Author(s): 中島 晶子，武藤 智，杉浦 正一郎，堀内 明，古謝 将之，井上 正浩，野間 康央，北村 香介，常盤 紫野，斎藤 恵介，吉井 隆、磯谷 周治，井手 久満，山口 雷蔵，堀江 重郎
  • Organizer: 第78回日本泌尿器科学会東部総会
  • Place of Presentation: 東京
• [Presentation] 反復する嚢胞感染に対して術前検討用腎三次元立体モデルを作成し開窓術を行ったADPKD患者の一例 (2013)
  • Author(s): 中島 晶子，武藤 智，杉浦 正一郎，堀内 明，古謝 将之，井上 正浩，野間 康央，北村 香介，常盤 紫野，斎藤 恵介，吉井 隆、磯谷 周治，井手 久満，山口 雷蔵，堀江 重郎
  • Organizer: 第43回日本腎臓学会東部学術大会
  • Place of Presentation: 東京
• [Presentation] 多発性嚢胞腎患者全国登録による多施設共同研究（J-PKDレジストリー研究）の登録時データの集計 (2013)
  • Author(s): 武藤 智，望月俊雄，土谷 健，奴田原紀久雄，西尾妙織，花岡一成，鶴屋和彦，石村栄治，成田一衛，香村衡一，乳原善文，堀江重郎
  • Organizer: 第56回日本腎臓学会学術総会
  • Place of Presentation: 東京
• [Presentation] ADPKDによる肝嚢胞患者を対象としたQOL調査 (2012)
  • Author(s): 武藤 智，望月俊雄，奴田原紀久雄，安藤昌彦，堀江重郎．
  • Organizer: 第55回日本腎臓学会学術総会
  • Place of Presentation: 横浜
• [Presentation] 常染色体優性多発性嚢胞腎患者向け漫画パンフレットの作成．第55回日本腎臓学会学術総会 (2012)
  • Author(s): 武藤 智，望月俊雄，堀江重郎．
  • Organizer: 第55回日本腎臓学会学術総会
  • Place of Presentation: 横浜
• [Book] 多発性嚢胞腎診療ガイドQ&A (進行性腎障害診療指針シリーズ) (2014)
  • Author(s): 松尾 清一, 堀江 重郎
  • Total Pages: 146
  • Publisher: 診断と治療社
• [Book] 今日の治療と看護（改訂第三版） (2013)
  • Author(s): 武藤 智，堀江重郎
  • Total Pages: 1493
  • Publisher: 南江堂
• [Book] Annual Review腎臓 (2013)
  • Author(s): 武藤 智，堀江重郎
  • Total Pages: 351
  • Publisher: 中外医学社