| Project/Area Number |
24592449
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Nagoya City University |
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Principal Investigator |
KUBOTA Hiroki 名古屋市立大学, 医学(系)研究科(研究院), 研究員 (10347403)
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| Co-Investigator(Kenkyū-buntansha) |
KOHRI Kenjiro 名古屋市立大学, その他の部局, 学長 (30122047)
HAYASHI Yutaro 名古屋市立大学, 大学院医学研究科, 准教授 (40238134)
SASAKI Shoichi 名古屋市立大学, 大学院医学研究科, 准教授 (50225869)
UMEMOTO Yukihiro 名古屋市立大学, 大学院医学研究科, 講師 (80381812)
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| Research Collaborator |
PARRINGTON John Oxford大学
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | Cyclooxygenase2 / Spermatogenesis / 造精機能障害 / Cox-2 / COX-2 |
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| Outline of Final Research Achievements |
In this study, we examined whether COX-2 was induced in impaired testes, and also investigated the possible role of COX in the testes using experimental cryptorchidism model mice. Five-week-old male mice underwent an operation to induce unilateral cryptorchidism, and they were then divided into three groups. Immunohistological staining and RT-PCR revealed that the expression of COX-2 was increased in the experimental cryptorchid testes. TdT-mediated dUTP-biotin nick end-labeling (TUNEL) staining revealed that the number of apoptotic cells was significantly increased by COX-2 inhibitor. However, the COX-1 inhibitor did not appear to affect spermatogenesis in the experimental cryptorchid testes. These results suggest that the COX-2 inhibitor provoked testicular damage in experimental cryptorchidism by inducing germ cell apoptosis. The expression of COX-2 might be induced to protect germ cells from heat stress caused by experimental cryptorchidism.
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