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Basic studies of therapeutic modality for polycystic kidney disease explored by epigenetic modification of cellular matrix-adhesion proteins

Research Project

Project/Area Number 24592450
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Urology
Research InstitutionNara Medical University

Principal Investigator

ISHIBASHI MIchio  奈良県立医科大学, 医学部, 研究員 (40107032)

Co-Investigator(Kenkyū-buntansha) HIGASHIHARA Eiji  杏林大学, 医学部, 教授 (00092312)
NAGAO Shizuko  藤田保健衛生大学, 医学部・疾患モデル教育研究センター, 准教授 (20183527)
CHIHARA Yoshitomo  奈良県立医科大学, 医学部, 講師 (40405395)
KOJIMA Naoto  京都薬科大学, 薬学部, 講師 (90420413)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords多発性嚢胞腎 / PCKラット / 細胞マトリックス / ベンゾイソフラノン誘導体 / ラットPCK / 細胞外マトリクス / DNAメチル化 / ベンゾイソフラノン化合物
Outline of Final Research Achievements

We studied the mechanism of progression of polycystic kidney disease explored by the pathological pictures of PCK rat kidneys of 5-week old male, treated with Benzoisofuranone derivatives for five weeks, and the epigenetic modification of cellular matrix-adhesion protein genes such as HIF-1, Galectin-3, DNMT1, Mlana, RTL1, Gypsy integrase-1 (Gin1) in frozen PCK kidney tissues. The pathology of beneficial findings of PCK rat kidney treated, showed stabilization of ductal tubules and that of peri-ductal capillary without sludging of blood, and minimization of cystic mass growing. The response of HIF-1 mRNA expression of frozen kidney was decreased with concomitant increase of DNA methylation level, which is benefical response of anti-fibrosis of PCK kidney. An increase of mRNA expression of Mlana, having function of angiogenesis, and a decrease of that of Gin1 were observed. Those findings suggested that an epigenetic modification played a role in the progression of PCK kidneys.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (1 results)

All 2012

All Journal Article (1 results)

  • [Journal Article] なし2012

    • Author(s)
      なし
    • Journal Title

      なし

      Volume: なし

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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