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Factors involved in an increase of the miscarriage rate with maternal age: Attempt to improve artificially oocyte quality in human

Research Project

Project/Area Number 24592455
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionHirosaki University

Principal Investigator

WATANABE Seiji  弘前大学, 医学(系)研究科(研究院), 助教 (10241449)

Research Collaborator TANAKA Atsushi  セントマザー産婦人科医院, 医学博士
MORI Marina  セントマザー産婦人科医院, 胚培養師
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords卵子の老化 / oocyte aging / 卵子成熟 / 染色体異常 / 初期発生 / 細胞周期 / 単為発生
Outline of Final Research Achievements

It is well known that an increase of the miscarriage rate and cytogenetically abnormal infants is related to maternal age. This phenomenon is called "oocyte aging". Immature human oocytes, which are obtained in the treatment of infertile female patients, are useful as a model of the oocytes obtained from aged females. We attempted to reveal mechanism of oocyte aging using the model oocytes. As a result, it becomes clear that oocyte development is improved when the oocytes are treated with a kind of chemical. The fact was also found that the improvement of oocyte quality was caused by an increase of cell cycle dependent proteins, which control chromosome distribution during cell division. Furthermore, cytogenetic analysis showed that expression of many genes altered in the model oocytes after the chemical treatment. As a next step, we are going to examine relationships between the genes and oocyte aging.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (19 results)

All 2015 2013 2012

All Presentation (19 results) (of which Invited: 1 results)

  • [Presentation] Effect of taxol on chromosomal cohesion and blastocyst formation rate in human oocytes2015

    • Author(s)
      Seiji Watanabe
    • Organizer
      IFFS International Meeting 2005
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2015-04-26 – 2015-04-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Effect of taxol on chromosome cohesion and blastocyst formation rate in human oocytes.2015

    • Author(s)
      Seiji Watanabe
    • Organizer
      第120回日本解剖学会総会・全国学術集会
    • Place of Presentation
      Kobe, Japan
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] ARTにおける卵子及び精子染色体異常2013

    • Author(s)
      渡邉誠二
    • Organizer
      生殖医学会東北支部会
    • Place of Presentation
      青森
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Ionomycin-Puromycin併用卵子活性化法の有用性に関する検討2013

    • Author(s)
      田中威づみ、加藤由香、髙橋如、中嶋美紀、赤星孝子、竹本洋一、 御木多美登、伊熊慎一郎、永吉基、田中温 、渡邉誠二
    • Organizer
      第31回 日本受精着床学会
    • Place of Presentation
      別府
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒトIVM卵子及びマウス卵子におけるオーロラ蛋白分布の比較2013

    • Author(s)
      渡邉誠二、田中威づみ、加藤由香、髙橋如、中嶋美紀、赤星孝子、竹本洋一、 御木多美登、伊熊慎一郎、永吉基、田中温
    • Organizer
      第31回 日本受精着床学会
    • Place of Presentation
      別府
    • Related Report
      2013 Research-status Report
  • [Presentation] イオノマイシン・ピューロマイシン併用法によるヒト卵子活性化能の検討2013

    • Author(s)
      田中威づみ、加藤由香、髙橋如、中嶋美紀、赤星孝子、竹本洋一、 御木多美登、伊熊慎一郎、永吉基、田中温 、渡邉誠二
    • Organizer
      第58回 日本生殖医学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
  • [Presentation] Rescue of aged oocytes using metaphase-II nuclear transfer with various kinds of fusion techniques2013

    • Author(s)
      A. Tanaka, I. Tanaka, M. Nagayoshi, H. Kusunoki and S. Watanabe
    • Organizer
      69th American Society for Reprocutive Medicine
    • Place of Presentation
      Boston(アメリカ)
    • Related Report
      2013 Research-status Report
  • [Presentation] Rescue of aged oocytes using metaphase-II nuclear transfer with various kinds of fusion techniques2013

    • Author(s)
      A. Tanaka, I. Tanaka, T. Miki, S. Ikuma, M. Nagayoshi, H. Kusunoki, S. Watanabe
    • Organizer
      The 9th Conference of the Pacific Rim Society for Fertility and Sterility
    • Place of Presentation
      Kobe
    • Related Report
      2013 Research-status Report
  • [Presentation] DOR救済の為の細胞質置換2013

    • Author(s)
      田中温, 田中威づみ, 永吉基, 楠比呂志, 渡邉誠二
    • Organizer
      第16回日本IVF学会
    • Place of Presentation
      神奈川
    • Related Report
      2013 Research-status Report
  • [Presentation] 電気融合法を用いない核置換法の開発2012

    • Author(s)
      田中温、永吉基、山本正孝、伊熊慎一郎、田中威づみ、竹本洋一、赤星孝子、楠比呂志、渡邉誠二
    • Organizer
      第69回 日本生殖医学会 九州支部会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] The future is now:oocyte plasmic exchange for aged oocytes2012

    • Author(s)
      田中温、永吉基、田中威づみ、楠比呂志、渡邉誠二
    • Organizer
      Mitochondrial Medicine 2012
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] 老化卵子救済のための第二成熟分裂中期(M-II)染色体置換における電気融合を使用しない染色体(直接)注入の臨床応用について2012

    • Author(s)
      田中温、永吉基、伊熊慎一郎、田中威づみ、竹本洋一、赤星孝子、楠比呂志、渡邉誠二
    • Organizer
      第30回 日本受精着床学会
    • Place of Presentation
      長崎
    • Related Report
      2012 Research-status Report
  • [Presentation] 卵子活性化能の比較2012

    • Author(s)
      田中温、永吉基、伊熊慎一郎、田中威づみ、竹本洋一、赤星孝子、加藤由香、楠比呂志、渡邉誠二
    • Organizer
      第30回 日本受精着床学会
    • Place of Presentation
      長崎
    • Related Report
      2012 Research-status Report
  • [Presentation] hCG投与後の未成熟卵子を体外培養したMII期卵子についての受精能の比較検討~採卵後6時間後と28時間後の比較検討~2012

    • Author(s)
      髙橋如、田中温、永吉基、伊熊慎一郎、田中威づみ、竹本洋一、赤星孝子、加藤由香、中嶋美紀、岡部美咲、楠比呂志、渡邉誠二
    • Organizer
      第30回 日本受精着床学会
    • Place of Presentation
      長崎
    • Related Report
      2012 Research-status Report
  • [Presentation] 着床前診断におけるM期染色体診断法の確立と有用性について2012

    • Author(s)
      渡邉誠二
    • Organizer
      第63回 染色体学会
    • Place of Presentation
      旭川
    • Related Report
      2012 Research-status Report
  • [Presentation] Development of a NEW tubal recanalization method using an ultrasonic tomography in the treatment of obstructed fallopian tubes.2012

    • Author(s)
      田中温、永吉基、伊熊慎一郎、田中威づみ、楠比呂志、渡邉誠二
    • Organizer
      68th ASRM (American Society for Reproductive Medicine)
    • Place of Presentation
      サンディエゴ( アメリカ)
    • Related Report
      2012 Research-status Report
  • [Presentation] Rescue of aged oocytes using metaphase-II nuclear transfer with various kinds of fusion techniques2012

    • Author(s)
      田中温、永吉基、田中威づみ、楠比呂志、渡邉誠二
    • Organizer
      68th ASRM (American Society for Reproductive Medicine)
    • Place of Presentation
      サンディエゴ( アメリカ)
    • Related Report
      2012 Research-status Report
  • [Presentation] 第二成熟分裂中期(M-II)染色体置換における電気融合を使用しない卵細胞質提供について2012

    • Author(s)
      田中温、永吉基、伊熊慎一郎、田中威づみ、竹本洋一、赤星孝子、楠比呂志、渡邉誠二
    • Organizer
      第57回 日本生殖医学会
    • Place of Presentation
      大阪
    • Related Report
      2012 Research-status Report
  • [Presentation] 電気刺激及びIonophore,Puromycinを用いた卵子活性化の検討2012

    • Author(s)
      加藤由香、田中温、永吉基、伊熊慎一郎、田中威づみ、竹本洋一、赤星孝子、髙橋如、中嶋美紀、渡邉誠二
    • Organizer
      第57回 日本生殖医学会
    • Place of Presentation
      大阪
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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