| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Immature oocytes retrieved from the ovaries can be maturated in vitro (IVM). If the IVM MII oocytes are enucleated and cryopreserved in liquid nitrogen, they can be used as a source of the SCNT as well as be used to establish the ntES cells. Developmental rate of the cloned embryo generated with IVM oocytes was significantly lower than that of the cloned embryo generated with fresh oocytes. In aged oocyte, low developmental rate was reported to be associated with mitochondrial abnormal distribution. Thus using the metaphase II spindle injection (MESI) method, we investigate the relationship between the spindle and mitochondrial distribution in the murine MII oocyte. The MII spindle might be a determination factor of the mitochondrial distribution in the fresh MII ooplasm, but mitochondria were no longer redistributed in the 1-d-o ooplasm, implying that the redistribution potential of mitochondria might be related to the function of the MII oocyte.
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