Analysis of lysophospholipid mediators in human follicle growth toward good-quality oocytes

• Project/Area Number: 24592468
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Nagoya University
• Principal Investigator: IWASE Akira 名古屋大学, 医学部附属病院, 病院教授 (20362246)
• Co-Investigator(Kenkyū-buntansha): GOTO Maki 名古屋大学, 医学部附属病院, 病院講師 (90378125)
• Co-Investigator(Renkei-kenkyūsha): NAKAMURA Tomoko 名古屋大学, 医学部附属病院, 病院助教 (40732681) ; NAKAHARA Tatsuo 名古屋大学, 医学部附属病院, 助教 (50534830)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 卵胞発育 / 顆粒膜細胞 / 脂質メディエーター / コレステロール合成 / S1P / GDF-9 / スフィンゴシン1リン酸 / コレステロール / FF-MAS

Outline of Final Research Achievements

An oocyte-derived growth factor, growth differentiation factor-9 upregulated the cholesterol-synthetic enzymes in human granulosa cells. We found the significant differences in the expression of the cholesterol-synthetic enzymes between luteinized granulosa cells derived from pregnant and non-pregnant follicles.
A lysophospholipid mediator, shingosine-1-phosphate (S1P) activated the PI3K-Akt pathway and stimulated proliferation of human granulosa cells. In addition, S1P inhibited H2O2-induced apoptosis of granulosa cells.

Research Products

• [Journal Article] CYP51A1 Induced by Growth Differentiation Factor 9 and Follicle-Stimulating Hormone in Granulosa Cells Is a Possible Predictor for Unfertilization (2014)
  • Author(s): Nakamura T, Iwase A, Bayasula B, Nagatomo Y, Kondo M, Nakahara T, Takikawa S, Goto M, Kotani T, Kiyono T, Kikkawa F
  • Journal Title: Reproductive Sciences Volume: in press
  • Peer Reviewed
• [Journal Article] Sphingosine-1-phosphate inhibits H2O2-induced granulosa cell apoptosis via the PI3K/Akt signaling pathway (2012)
  • Author(s): Nakahara T, Iwase A, Nakamura T, Kondo M, Bayasula, Kobayashi H, Takikawa S, Manabe S, Goto M, Kotani T, Kikkawa F.
  • Journal Title: Fertility and Sterility Volume: 98 Issue: 4 Pages: 1001-8
  • DOI: 10.1016/j.fertnstert.2012.06.008
  • Peer Reviewed
• [Presentation] Proliferation and apoptosis of granulosa cell: A link to the folliculogenesis
  • Author(s): Akira Iwase
  • Organizer: The 9th Conference of the Pacific Rim Society for Fertility and Sterility
  • Place of Presentation: 神戸国際会議場（神戸）
  • Invited