Role of cathepsin E in patients with recurrent miscarriage
| Project/Area Number |
24592479
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | Nagoya City University |
|---|
Principal Investigator |
OZAKI Yasuhiko 名古屋市立大学, 医学(系)研究科(研究院), 准教授 (50254280)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 不育症 / 習慣流産 / カテプシン / プロテアーゼ / マクロファージ / 着床 / 抗リン脂質抗体症候群 / プロテオリシス / メタロプロテアーゼ / カルパイン / 胎盤 / 子宮内胎児発育遅延 / カルパスタチン / 妊娠高血圧症候群 |
|---|
| Outline of Final Research Achievements |
We investigated the existence and contribution of cathepsin E (Cat E) in early pregnancy of patients with recurrent miscarriage. With informed consent, sera, cervical mucous, decidua and villi were surgically collected from patients with recurrent miscarriage. An activity of Cat E was detected in sera and cervical mucous. The Cat E activity in cervical mucous of patients with spontaneous miscarriages was lower than that of successful pregnancy. By immunohistochemistry, the staining of Cat E was detected in the cytoplasm of stromal and epithelial cells in decidua and villi. In decidua, the staining of Cat E was observed in CD14 positive cells and CD68 positive cells. Western blot analysis showed Cat E expression in decidual macrophages. By addition of LPS and IFN-γ, Cat E expression in cell lysate of decidual macrophages was decreased.
The results suggested that activity of Cat E produced by decidual macrophages plays an important role in the mechanism of early pregnancy.
|
Report
(4 results)
Research Products
(16 results)
-
-
[Journal Article] Cathepsin E in mammary gland: Repression of cathepsin E expression increases the risk of mammary carcinogenesis and links to poor prognosis in breast cancer.2013
Author(s)
Tomoyo Kawakubo, Atsushi Yasukochi, Tatsuya Toyama, Satoru Takahashi, Kuniaki Okamoto, Takayuki Tsukuba, Seiji Nakamura, Yasuhiko Ozaki, Koichi Nishigaki, Hiroko Yamashita, and Kenji Yamamoto
-
Journal Title
Carcinogenesis
Volume: 00
Issue: 3
Pages: 1-13
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
[Presentation] Role of calpain in human placenta of patients with feta1 growth restriction2014
Author(s)
Shinobu Goto, Yasuhiko Ozaki, Satomi Kurakane, Yuki Obayashi, Kyoko Kumagai, Tamao Kitaori, Nobuhiro Suzumori, Mayumi Sugiura-Ogasawara
Organizer
The 46th International Congress on Pathophysiology of Pregnancy and The 35th Meeting of Japan Society for the Study of Hypertension in Pregnancy
Place of Presentation
Tokyo, Japan
Year and Date
2014-09-18 – 2014-09-20
Related Report
-
-
-
-
-
-
-
-
-
-
