| Project/Area Number |
24592480
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
KITAWAKI Jo 京都府立医科大学, 医学(系)研究科(研究院), 教授 (00204925)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | ヘテロ接合性の消失 / 悪性形質転換 / 子宮内膜症から発生する卵巣癌 / 突然変異 / 産婦人科学 / 生殖医学 / 子宮内膜症 / 卵巣癌 |
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| Outline of Final Research Achievements |
To investigate the genetic alterations during transformation of ovarian endometriosis into cancer, we analysed loss of heterozygosity (LOH) and mutations of tumor-related genes. Each paraffin-embedded section was microdissected to isolate the endometriotic epithelium, transitional epithelium and cancer cells. Extracted DNA was analysed for LOH events and mutations. With 13 microsatellite markers on 6 chromosomes, we detected 31 and 8 LOH events in OCEMs and in solitary ovarian endometriosis, respectively. Only two point mutations of PTEN were detected in cancer cells in one case. Our study suggested that accumulated LOH events on some chromosome loci may be involved in malignant transformation of endometriosis, while mutations in certain tumor-related genes are not.
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