Analysis of human endometrial epithelial cell dynamics during implantation

• Project/Area Number: 24592484
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Keio University
• Principal Investigator: UCHIDA HIROSHI 慶應義塾大学, 医学部, 講師 (90286534)
• Co-Investigator(Kenkyū-buntansha): MARUYAMA Tetsuo 慶應医義塾大学, 医学部, 講師 (10209702) ; MASUDA Hirotaka 慶應医義塾大学, 医学部, 助教 (80317198)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 生殖医学 / 着床 / 細胞運動 / 細胞接着 / 子宮内膜上皮細胞 / グリコデリン

Outline of Final Research Achievements

Knowledge of human implantation mechanism is essential for assisted reproductive technology. It is known that human endometrial epithelial cells fall into apoptosis and disappear for embryo passing through. This study demonstrated that a number of human endometrial epithelial cells efferent move away from the center of embryo attachment, in association with cell apoptosis of small number of endometrial epithelial cells during implantation in vitro.

Research Products

• [Journal Article] Serum estradiol level during withdrawal bleeding as a predictive factor for intermittent ovarian function in women with primary ovarian insufficiency (2015)
  • Author(s): Miyazaki K, Miki F, Uchida S, Masuda H, Uchida H, Maruyama T
  • Journal Title: Endocrine Journal Volume: 62 Issue: 1 Pages: 93-99
  • DOI: 10.1507/endocrj.EJ14-0189
  • NAID: 130004694069
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed / Open Access
• [Journal Article] 目で見る母児境界面の生理と病理． (2015)
  • Author(s): 内田 浩，丸山哲夫，田中 守
  • Journal Title: HORMONE FRONTIER IN GYNECOLOGY Volume: 22 Pages: 4-7
• [Journal Article] Molecular analysis of a mutated FSH receptor detected in a patient with spontaneous ovarian hyperstimulation syndrome. (2013)
  • Author(s): Uchida S, Uchida H, Maruyama T, Kajitani T, Oda H, Miyazaki K, Maki Kagami, Yoshimura Y
  • Journal Title: PLoS One. Volume: VOL. 8 Issue: 9 Pages: e75478-e75478
  • DOI: 10.1371/journal.pone.0075478
  • Peer Reviewed
• [Journal Article] Possible involvement of nerve growth factor in dysmenorrhea and dyspareunia associated with endometriosis. (2013)
  • Author(s): Kajitani T, Maruyama T, Asada H, Uchida H, Oda H, Nishikawa-Uchida S, Miyazaki K, Arase T, Ono M, Yoshimura Y
  • Journal Title: Endocr J. Volume: 60 Pages: 1155-64
  • NAID: 130004770386
  • Peer Reviewed
• [Journal Article] Achievement of pregnancies in women with primary ovarian insufficiency using close monitoring of follicle development: case reports. (2013)
  • Author(s): Maruyama T, Miyazaki K, Uchida H, Uchida S, Masuda H, Yoshimura Y
  • Journal Title: Endocr J. Volume: VOL. 60 Pages: 791-7
  • NAID: 10031184548
  • Peer Reviewed
• [Journal Article] Human uterine stem/ progenitor cells : Implications for uterine physiology and pathology (2013)
  • Author(s): Maruyama T, Miyazaki K, Masuda H, Ono M, Uchida H, Yoshimura Y
  • Journal Title: Placenta Volume: 27 Pages: 77-81
  • DOI: 10.1016/j.placenta.2012.12.010
  • Peer Reviewed
• [Journal Article] Achievement of pregnancies in women with primary ovarian insufficiency using close monitoring of follicle development: case reports. (2013)
  • Author(s): Maruyama T, Miyazaki K, Uchida H, Uchida S, Masuda H, Yoshimura Y
  • Journal Title: Endocrine Journal. Volume: -
  • NAID: 10031184548
  • Peer Reviewed
• [Journal Article] Stem cell-like differentiation potentials of endometrial side population cells as revealed by a newly developed in vivo endometrial stem cell assay (2012)
  • Author(s): Miyazaki K, Maruyama T, Masuda H, Yamasaki A, Uchida S, Oda H, Uchida H, Yoshimura Y
  • Journal Title: PLoS One Volume: 7(12) Issue: 12 Pages: e50749-e50749
  • DOI: 10.1371/journal.pone.0050749
  • Peer Reviewed
• [Journal Article] Psychological adjustment and psychosocial stress among Japanese couples with a history of recurrent pregnancy loss (2012)
  • Author(s): M.Kagami, T.Maruyama, T.Koizumi, K.Miyazaki, S.Nishikawa-Uchida, H.Oda, H.Uchida, D.Fujisawa, N.Ozawa, L.Schmidt, Y.Yoshimura
  • Journal Title: Human Reproduction Volume: 27 Issue: 3 Pages: 787-794
  • DOI: 10.1093/humrep/der441
  • Peer Reviewed
• [Presentation] Decellularization of the rat uterus yielded scaffold capable of tissue regeneration without immune rejection upon allotransplantation. (2015)
  • Author(s): Kaoru Miyazaki, Yuki Ueno, Hirotaka Masuda, Hiroshi Uchida, Tetsuo Maruyama
  • Organizer: 62nd Society for Reproductive Investigation (SRI)
  • Place of Presentation: San Francisco (USA)
  • Year and Date: 2015-03-25 – 2015-03-28
• [Presentation] 同種異系移植されたラット子宮の脱細胞化マトリックスは、免疫拒絶を受けることなく組織再生を促進する． (2015)
  • Author(s): 宮崎 薫，上野友輝，升田博隆，内田 浩，丸山哲夫
  • Organizer: 第14回日本再生医療学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2015-03-19 – 2015-03-21
• [Presentation] 経卵管采式卵管鏡(Salpingoscopy)による卵管内環境のイメージング～受精・胚成長に必要な卵管内生殖環境の新しい評価法を目指して～． (2014)
  • Author(s): 太田邦明，佐藤健二，飯野孝太郎，宮崎 薫，小川誠司，佐藤 卓，升田 博隆，内田 浩，浜谷敏生，丸山哲夫，末岡 浩，田中 守
  • Organizer: 第59回日本生殖医学会
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2014-12-04 – 2014-12-05
• [Presentation] 完全中隔子宮に対する子宮鏡下手術の新しい試み ― 頸部中隔切断の有用性 ― ． (2014)
  • Author(s): 安康真由香，佐藤健二，升田博隆，太田邦明，宮崎 薫，小川誠司，佐藤 卓，内田 浩，浜谷敏生，丸山哲夫，末岡 浩，田中 守
  • Organizer: 第59回日本生殖医学会
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2014-12-04 – 2014-12-05
• [Presentation] 卵巣過剰刺激症候群に対する内分泌学的加療により卵巣出血を来した一例． (2014)
  • Author(s): 三木史恵，内田 浩，宮崎 薫，太田邦明，内田明花，升田博隆，丸山哲夫，田中 守
  • Organizer: 第15回日本内分泌学会関東甲信越支部学術集会
  • Place of Presentation: ラフレさいたま（埼玉県さいたま市）
  • Year and Date: 2014-09-05 – 2014-09-06
• [Presentation] 不育症診療おける化学妊娠の取り扱いに関する検討． (2014)
  • Author(s): 三木史恵，丸山哲夫，内田明花，各務真紀，升田博隆，内田 浩，吉村泰典
  • Organizer: 第32回日本受精着床学会
  • Place of Presentation: ハイアットリージェンシー東京（東京都新宿区）
  • Year and Date: 2014-07-31 – 2014-08-01
• [Presentation] Reconstruction and regeneration of the rat uterus through novel decellularization and recellularization techniques. (2014)
  • Author(s): Kaoru Miyazaki, Tetsuo Maruyama, Hirotaka Masuda, Naoko Hida, Hiroshi Uchida, Yasunori Yoshimura
  • Organizer: 12th International Society for Stem Cell Research (ISSCR)
  • Place of Presentation: Vancouver (Canada)
  • Year and Date: 2014-06-18 – 2014-06-21
• [Presentation] 原因不明不育症女性における潜在性甲状腺機能低下症の検討． (2014)
  • Author(s): 内田明花，丸山哲夫，三木史恵，各務真紀，宮崎 薫，升田博隆，内田 浩，吉村泰典
  • Organizer: 第87回日本内分泌学会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] 原因不明不育症には潜在性甲状腺機能低下症の亜集団が存在する． (2014)
  • Author(s): 内田明花，丸山哲夫，三木史恵，各務真紀，宮崎 薫，升田博隆，内田 浩，吉村泰典，青木大輔
  • Organizer: 第66回日本産科婦人科学会
  • Place of Presentation: 国際フォーラム（東京都千代田区）
  • Year and Date: 2014-04-18 – 2014-04-20
• [Presentation] 不育症学級を活用した不育症患者夫婦へのメンタルヘルスケアの有効性に関する検討． (2014)
  • Author(s): 各務真紀，丸山哲夫，小泉智恵，越川和子，菅沼真樹，内田明花，宮崎 薫，升田博隆，内田 浩，吉村泰典
  • Organizer: 第66回日本産科婦人科学会
  • Place of Presentation: 国際フォーラム（東京都千代田区）
  • Year and Date: 2014-04-18 – 2014-04-20
• [Book] 生殖医療ポケットマニュアル (2014)
  • Author(s): 内田 浩
  • Total Pages: 452
  • Publisher: 医学書院