Expression of vascular growth factor and hypoxia responsive factor in preeclampsia placenta

• Project/Area Number: 24592486
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Nihon University
• Principal Investigator: YAMAMOTO Tatsuo 日本大学, 医学部, 教授 (40167721)
• Co-Investigator(Kenkyū-buntansha): CHISHIMA Fumihisa 日本大学, 医学部, 准教授 (50277414) ; KUNO Souichiro 日本大学, 医学部, 助教 (30350002) ; ICHIKAWA Go 日本大学, 医学部, 助教 (80599994)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: PlGF / VEGF / sFlt-1 / HIF-1alpha / MTF-1 / HO-1 / sEndoglin / AT1-AA / 妊娠高血圧症候群 / 妊娠高血圧腎症 / placenta growth factor / ｓFlt-1 / 血清因子 / 低酸素応答分子

Outline of Final Research Achievements

In order to investigate pathophysiology in preeclampsia, we studied the effect of preeclampsia sera on their expression from trophoblast derived cells and the expression of PlGF, sFlt-1,MTF-1,HO-1 and HIF-1α mRNAs in placenta taken from preeclampsia. The changes of PlGF mRNA in preeclampsia placenta may relate to serum factor and the expression of MTF-1 and HIF-αmRNA.　 The changes of sFlt-1mRNA may relate to serum factor and the expression of HIF-alpha mRNA.　We suggested that not only hypoxia but also serum factor may play a role in the levels of PlGF and sFlt-1 in preeclampsia placenta.

Research Products

• [Journal Article] Autoantibodies Isolated from Patients with Preeclampsia Induce Soluble Endoglin Production from Trophoblast Cells Via Interactions with Angiotensin II Type 1 Receptor. (2015)
  • Author(s): Kobayashi Y, Yamamoto T, Chishima F, Takahashi H, Suzuki M. Autoantibodies Isolated from Patients with Preeclampsia Induce Soluble Endoglin Production from Trophoblast Cells Via Interactions with Angiotensin II Type 1 Receptor.
  • Journal Title: Am J Reprod Immunol. Volume: 73(4) Issue: 4 Pages: 285-291
  • DOI: 10.1111/aji.12340
  • Peer Reviewed
• [Journal Article] AT1-AAと妊娠高血圧症候群 (2014)
  • Author(s): 小林祐介、新屋芳里、中山琢生、高橋英幹、山本樹生
  • Journal Title: 周産期医学 Volume: 44(11) Pages: 1445-1449
• [Journal Article] Expression of placenta growth factor, soluble fms-like tyrosine kinase-1, metal-responsive transcription factor-1, heme oxygenase 1 and hypoxia inducible factor-1α mRNAs in pre-eclampsia placenta and the effect of pre-eclampsia sera on their expression of choriocarcinoma cells. (2014)
  • Author(s): Maebayashi Asanuma A1, Yamamoto T, Azuma H, Kato E, Yamamoto N, Murase T, Chishima F, Suzuki M.
  • Journal Title: J Obstet Gynaecol Res. Volume: ４０（１０） Issue: 10 Pages: 2095-2103
  • DOI: 10.1111/jog.12462
  • Peer Reviewed
• [Journal Article] Expression of PlGF,sFlt-1,metal-responsive transcription factor-1, hemeoxygenase and hypoxia inducible factor-1α mRNA in preeclampsia placenta and the effect of preeclampsia sera on their expression of choriocarcinoma cells (2014)
  • Author(s): Maebayashi A,Yamamoto T,Azuma H et al.
  • Journal Title: Journal of Obstetrics and Gynecology Reseach Volume: 0 Pages: 0-0
  • Peer Reviewed
• [Journal Article] Prediction of preeclampsia using angiogenic and antiangiogenic factors (2013)
  • Author(s): Yamamoto T, Chishima F, Matsuura M
  • Journal Title: Hypertention Research in Pregnancy Volume: 2 Pages: 1-5
  • NAID: 130004638239
  • Peer Reviewed
• [Presentation] 妊娠高血圧腎症におけるsFlt-1の発現に影響を与える因子の解析 (2015)
  • Author(s): 前林 亜紀、新屋 芳里、中山 琢生、高橋 英幹、東 裕福、久野 宗一郎、千島 史尚、山本 樹生
  • Organizer: 第67回日本産科婦人科学会学術講演会
  • Place of Presentation: パシフィコ横浜・神奈川県・横浜市
  • Year and Date: 2015-04-10 – 2015-04-12
• [Presentation] AT1-AAの妊娠初期絨毛細胞よりのsoluble Endoglin産生におけるサイトカインの関与 (2015)
  • Author(s): 小林 祐介、新屋 芳里、中山 琢生、高橋 英幹、村瀬 隆之、千島 史尚、山本 樹生
  • Organizer: 第６７回日本産科婦人科学会総会
  • Place of Presentation: パシフィコ横浜・神奈川県・横浜市
  • Year and Date: 2015-04-10 – 2015-04-12
• [Presentation] Expression of PlGF, sFlt, MTF-1, HO-1 and HIF-1 alpha mRNAs in preeclampsia placenta and effect of preeclampsia sera on their expression of choriocarcinoma cells (2014)
  • Author(s): Aki Maebayashi (Asanuma), Tatsuo Yamamoto ,Hiromitu Azuma, Erina Kato Souichirou Kuno, Takayuki Murase, Fumihisa Chishima, Manami Suzuki
  • Organizer: 46th International Congress on Pathophysiology of Pregnancy
  • Place of Presentation: 京王プラザホテル・東京都新宿区
  • Year and Date: 2014-09-18 – 2014-09-20
• [Presentation] 妊娠高血圧腎症患者血清中IgGの妊娠初期絨毛細胞よりのsoluble Endoglin, Cytokaine産生に対する影響 (2014)
  • Author(s): 小林 祐介、仲尾 岳大、東 裕福、中村 晃和、市川 剛、村瀬 隆之、千島 史尚、山本 樹生
  • Organizer: 第66回日本産科婦人科学会学術講演会
  • Place of Presentation: 東京国際フォーラム・東京都・千代田区
  • Year and Date: 2014-04-18 – 2014-04-20
• [Presentation] 妊娠高血圧症候群における重症化、児の予後の推定のためのsFlt-1、PlGFおよびsFlt-1/PlGF比の可能性について (2014)
  • Author(s): 新屋芳里 小林祐介 高橋英幹 中村晃和 佐々木重胤 松浦眞彦 山本樹生
  • Organizer: 第６６回日本産科婦人科学会総会
  • Place of Presentation: 東京
• [Presentation] 妊娠高血圧腎症患者血清中IgGの妊娠初期絨毛細胞よりのsoluble Endoglin産生に対する影響 (2013)
  • Author(s): 小林祐介、東裕福、仲尾岳大、中村晃和、久野宗一郎、村瀬隆之、千島史尚、山本樹生
  • Organizer: 第６５回日本産科婦人科学会総会
  • Place of Presentation: 札幌
• [Presentation] 妊娠高血圧腎症患者血清中IgGの妊娠絨毛細胞よりのsoluble Endoglin、TGF-β産生に対する影響 (2013)
  • Author(s): 小林祐介、仲尾岳大、東裕福、中村晃和、市川 剛、久野宗一郎、村瀬隆之、千島史尚、
  • Organizer: 第２８回日本生殖免疫学会
  • Place of Presentation: 兵庫
• [Presentation] 妊娠高血圧腎症患者血清IgG分画の絨毛癌細における可溶性エンドグリン産生に対する影響の検討 (2012)
  • Author(s): 小林祐介、東裕福、中村晃和、林 忠佑、市川 剛、千島史尚、小林真美、山本樹生
  • Organizer: 第２７回 日本生殖免疫学会
  • Place of Presentation: 大阪
• [Presentation] 妊娠高血圧腎症患者血清中IgGの絨毛細胞よりのsoluble Endoglin産生に対する影響
  • Author(s): 小林祐介、東裕福、仲尾岳大、中村晃和、久野宗一郎、村瀬隆之、千島史尚、山本樹生
  • Organizer: 第２３回 腎と妊娠研究会
  • Place of Presentation: 筑波