IgG transport mechanism of Fc gamma RIIb-containing compartments across the placental barrier

• Project/Area Number: 24592489
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Ochanomizu University
• Principal Investigator: ISHIKAWA Tomoko お茶の水女子大学, プロジェクト教育研究院, 特任准教授 (70212850)
• Co-Investigator(Kenkyū-buntansha): FUJIWARA Yoko お茶の水女子大学, 基幹研究院, 教授 (50293105)
• Co-Investigator(Renkei-kenkyūsha): TAKIZAWA Toshihiro 日本医科大学, 大学院医学研究科, 教授 (90271220) ; KIKUCHI Kunio 日本医科大学, 医学部, 講師 (70374676)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 細胞・組織 / 小胞輸送 / 免疫 / 栄養

Outline of Final Research Achievements

We performed in vitro bio-imaging analysis of IgG trafficking by FcγRIIb-containing compartments and elucidated that FcγRIIb participates in maternal IgG trafficking of placental endothelial cells and RAB3D plays a role in regulating intracellular dynamics of the FcγRIIb-containing compartments. Furthermore, we attempted to develop animal model and confirmed FcγRIIb expression level in hepatic sinusoidal endothelial cells during the diet-induced hepatic disorders.

Research Products

• [Journal Article] Fc gamma receptor IIb participates in maternal IgG trafficking of human placental endothelial cells. (2015)
  • Author(s): Ishikawa T, Takizawa T, Iwaki J, Mishima T, Ui-Tei K, Takeshita T, Matsubara S, Takizawa T.
  • Journal Title: nt. J. Mol. Med. Volume: 35 Issue: 5 Pages: 1273-1789
  • DOI: 10.3892/ijmm.2015.2141
  • Peer Reviewed / Open Access
• [Journal Article] Expression of flotillins in the human placenta: potential implications for placental transcytosis. (2013)
  • Author(s): Walton JR, Frey HA, Vandre DD, Kwiek JJ, Ishikawa T, Takizawa T, Robinson JM, Ackerman WE 4th.
  • Journal Title: Histochem Cell Biol Volume: 139 Issue: 3 Pages: 487-500
  • DOI: 10.1007/s00418-012-1040-2
  • Peer Reviewed
• [Presentation] 食事誘導性肝障害の発症過程における抑制型Fcg受容体の発現変動 (2016)
  • Author(s): 石川朋子、柴田彩霞、池上寛子、藤原葉子
  • Organizer: 第70回日本栄養・食糧学会大会
  • Place of Presentation: 武庫川女子大学（兵庫）
  • Year and Date: 2016-05-13
• [Presentation] グルテン食摂取ラットにおける抑制性Fcg受容体の発現動態の解析 (2015)
  • Author(s): 石川朋子、柴田彩霞、藤原葉子
  • Organizer: 第62回日本栄養改善学会各術総会
  • Place of Presentation: 福岡国際会議場（福岡）
  • Year and Date: 2015-09-24
• [Presentation] Tocotorienol improves the glucose tolerance in obese mice induced by high fat feeding (2015)
  • Author(s): H. Sato, Y. Kawamura, S. Tomino, M. Sonoda, I. Ichi, Y. Sone, T. Ishikawa, Y. Fujiwara
  • Organizer: 12th Asian Congress of Nutrition
  • Place of Presentation: Pacifico Yokohama (Kanagawa・Yokohama)
  • Year and Date: 2015-05-16
• [Presentation] 高脂肪食誘導性肥満マウスにおいて食餌性トコトリエノールが膵内分泌細胞に及ぼす影響 (2014)
  • Author(s): 石川朋子、川村悠貴、富野志穂、園田麻里子、市育代、曽根保子、藤原葉子
  • Organizer: 第55回日本組織細胞化学会総会・学術集会
  • Place of Presentation: 松本市中央公民館（長野・松本）
  • Year and Date: 2014-09-27
• [Presentation] Three-dimensional analysis of the onset of regional heterogeneity of hepatocytes and microvascular architecture in shrew hepatic lobules (2013)
  • Author(s): T. Ishikawa
  • Organizer: 20th International Congress of Nutrition
  • Place of Presentation: Congress Centre, Granada, SPAIN
• [Presentation] Fc Gamma Receptor IIb (FCGR2B2) Plays An Important Role In IgG Trafficking Of Human Placental Endothelial Cells (2012)
  • Author(s): To. Takizawa
  • Organizer: 14th International Congress of Histochemistry and Cytochemistry
  • Place of Presentation: Kyoto
• [Presentation] 胎盤血管内皮細胞においてRAB3はIgG輸送体であるIIb型Fc受容体を含む小胞の輸送能を調節している (2012)
  • Author(s): 瀧澤俊広
  • Organizer: 第27回日本生殖免疫学会学術集会
  • Place of Presentation: 大阪