Biological role of ZFP36 in gynecologic malignancies

• Project/Area Number: 24592497
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Hokkaido University
• Principal Investigator: HOSAKA Masayoshi 北海道大学, 大学病院, 助教 (20374390)
• Co-Investigator(Kenkyū-buntansha): WATARI Hidemichi 北海道大学, 北海道大学病院, 講師 (10344508) ; SAKURAGI Noriaki 北海道大学, 大学院医学研究科, 教授 (70153963)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: cervical cancer / CIN / HPV / ZFP36 / IMP3 / 子宮頸癌 / ZFP-36 / 免疫組織化学

Outline of Final Research Achievements

We aimed to examine the expression of RNA-binding proteins (IMP3 and ZFP36) in HPV-16-positive cervical intraepithelial neoplasia (CIN), and squamous cell carcinoma (SCC), and investigate whether their expression is correlated with disease progression of CIN2/3. Immunohistochemistry showed that higher expression of IMP3, lower expression of ZFP36 was more frequently observed in CIN3 and SCC than CIN1/2. Among CIN2/3, cases with higher IMP3 expression progressed to higher-grade lesions, whereas cases with lower IMP3 expression did not progress.We conclude that increased expression of IMP3 and/or reduced expression of ZFP36 may play a role in HPV-16-related cervical carcinogenesis. Immunohistochemical expression of IMP3 might be a useful marker to predict progression of HPV16-positive CIN2/3 to higher-grade lesions.

Research Products

• [Journal Article] Multivariate prognostic analysis on the patients with adenocarcinoma of the uterine cervix treated with radical hysterectomy and systematic lymphadenectomy. (2013)
  • Author(s): Kato T, Watari H*,Takeda M, Hosaka M, Mitamura T, Kobayashi N, Sudo S, Kaneuchi M, Kudo M, Sakuragi N.
  • Journal Title: Journal of Gynecologic Oncology Volume: 24 Issue: 3 Pages: 222-228
  • DOI: 10.3802/jgo.2013.24.3.222
  • Peer Reviewed
• [Journal Article] 子宮体がんにおけるリンパ節郭 清術の意義とその手技 (2013)
  • Author(s): 保坂昌芳、金内優典、渡利英道
  • Journal Title: 産婦人科の実際 Volume: 62 Pages: 39-43
• [Journal Article] Incidence risk of cervical intraepithelial neoplasia 3 or more severe lesions is a function of human papillomavirus genotypes and severity of cytological and histological abnormalities in adult Japanese women. (2013)
  • Author(s): Hosaka M, Fujita H, Hanley SJB, Sasaki T, Shirakawa Y, Abiko M, Kudo M, Kaneuchi M, Watari H, Kikuchi K, Sakuragi N.
  • Journal Title: International Journal of Cancer Volume: 132 Issue: 2 Pages: 327-334
  • DOI: 10.1002/ijc.27680
  • Peer Reviewed
• [Journal Article] Clinical efficacy of paclitaxel/cisplatin as an adjuvant chemotherapy for patients with cervical cancer who underwent radical hysterectomy and systematic lymphadenecctomy (2012)
  • Author(s): Hosaka M, Watari H, Kato T, Odagiri T, Konno Y, Endo D, Mitamura T, Kikawa S, Suzuki Y, Sakuragi N.
  • Journal Title: Journal of Surgical Oncology Volume: 105 Issue: 6 Pages: 612-616
  • DOI: 10.1002/jso.22136
  • Peer Reviewed
• [Presentation] 子宮頚部異形成（CIN）の進展とIMP3、ZFP36発現との関連について (2015)
  • Author(s): 嶋田知紗、保坂昌芳、渡利英道、山口正博、福本 俊、遠藤大介、井平 圭、金野陽輔、加藤達矢、工藤正尊、水上尚典、櫻木範明
  • Organizer: 第67回日本産科婦人科学会学術講演会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2015-04-10 – 2015-04-12
• [Book] OGS Now 13機能温存の手術―疾患治療と妊娠・性機能の予後に配慮して― (2013)
  • Author(s): 渡利英道、保坂昌芳、櫻木範明
  • Total Pages: 168
  • Publisher: メジカルビュー社