| Project/Area Number |
24592498
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | Yamagata University (2014)
Tohoku University (2012-2013) |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SUSUKI Fumihiko 東北大学, 医学(系)研究科(研究院), 助教 (20400343)
YOSHINAGA Kousuke 東北大学, 医学(系)研究科(研究院), 非常勤講師 (40343058)
TANNO Sumika 東北大学, 医学(系)研究科(研究院), 非常勤講師 (60509595)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
|---|
| Keywords | 子宮体癌 / 漿液性腺癌 / マイクロRNA / マイクロアレイ / 薬剤耐性 |
|---|
| Outline of Final Research Achievements |
To elucidate mechanisms of chemo-resistance for platinum and taxane from the aspect of microRNA (miRNA), we used USPC-1 and constructed its chemo-resistant variant PTX-R and CDDP-R. By microRNA microarray analysis, we focused on let-7c. We confirmed that the expression of let-7c was decreased significantly in both drug-resistant cells (P<0.05). Cell viability was decreased after let-7c mimic transfection to PTX-R followed by PTX treatment and similar transfection to CDDP-R followed by CDDP treatment. HMGA2 is known as the most famous target of let-7c and let-7c mimic transfection revealed decreased expression of HMGA2 (p<0.05). ABC transporters such as ABCB1, ABCC1, ABCC2, ABCG2, are the important molecules in the drug resistance, and the expression of ABCC1 was decreased significantly by let-7c inhibitor transfection to parental cells (p<0.05). These data shows that let-7c may involve in drug resistance of PTX and CDDP via HMGA2 or ABCC1 in USC.
|
