Comprehensive transcriptome analysis of uterine papillary serous carcinoma using next generation sequencer.

• Project/Area Number: 24592499
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Tohoku University
• Principal Investigator: HANEDA Ken 東北大学, 大学病院, 助手 (10622433)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Fumihiko 東北大学, 大学院医学系研究科, 助教 (20400343) ; SUZUKI Kichiya 東北大学, 東北メディカル・メガバンク機構, 准教授 (30422116)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 漿液性子宮体がん / マイクロRNA / 次世代シークエンサー / 漿液性子宮体癌

Outline of Final Research Achievements

Uterine papillary serous carcinoma (UPSC), unlike type I endometrial cancer, represents myometrial invasion and lymph node metastasis, even in the early stage of uterine cancer. It has been characterized by a high recurrence rate and a poor prognosis in gynecological malignant tumors. However, the occurrence and development mechanisms of UPSC are still unknown. Thus, in this study, we focused on the UPSC transcriptome. By using next-generation sequencing technology and DNA microarray technology, we attempt to analyze the specific transcriptome in tissues and serum to understand the molecular mechanisms and signaling pathways controlling the development. This study provides a perspective on the expression profile of UPSC, which will be a contribution in the molecular target therapy and a novel biomarker discovery in the future.

Research Products

• [Journal Article] Decreased Expression of 14-3-3σ is Predictive of Poor Prognosis for Patients with Human Uterine Papillary Serous Carcinoma. (2013)
  • Author(s): Suzuki F, Nagase S, Suzuki K, Oba E, Hiroki E, Matsuda Y, Akahira J, Nishigori H, Sugiyama T, Otsuki T, Yoshinaga K, Takano T, Niikura H, Ito K, Sasano H, Yaegashi N.
  • Journal Title: Tohoku J Exp Med Volume: 231 Pages: 193-199
  • NAID: 130004720755
  • Peer Reviewed
• [Journal Article] DNA demethylation-dependent AR recruitment and GATA factors drive Rhox5 homeobox gene transcription in the epididymis. (2012)
  • Author(s): Bhardwaj A, Song HW, Beildeck M, Kerkhofs S, Castoro R, Shanker S, De Gendt K, Suzuki K, Claessens F, Issa JP, Orgebin-Crist MC, Wilkinson MF
  • Journal Title: Mol Endocrinol Volume: 26 Pages: 538-549
  • Peer Reviewed