The role of epigenetic effector polycomb group gene in ovarian cancers

Research Project

Project/Area Number	24592502
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Obstetrics and gynecology
Research Institution	Yamagata University
Principal Investigator	SUDO Takeshi 山形大学, 医学部, 医員 (70466605)
Co-Investigator(Kenkyū-buntansha)	TAKAHASHI Toshifumi 山形大学, 医学部, 講師 (20302292) KURACHI Hirohisa 山形大学, 医学部, 非常勤講師 (40153366) OHTA Tsuyoshi 山形大学, 医学部, 助教 (50375341)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	卵巣癌 / 癌幹細胞 / ポリコーム複合体 / 薬剤感受性 / ハニカム膜 / エピジェネティク / 薬剤耐性
Outline of Final Research Achievements	We identified cancer stem-like cells (CSLC) in human ovarian cancer cell line A2780 by stem cell culture methods. The expression of polycomb group (PcG), which are epigenetic effector, showed no difference between parental A2780 and CSLC. Although cancer stem cells show the resistance to chemotherapeutic agent, A2780 CSLC did not have the ability of cisplatin resistance. Honeycomb films (3D porous scaffold) fabricated from various biodegradable polymers can amplify the cancer stem cells. Honeycomb-patterned polyurethane films inhibited cell proliferation compared with control tissue culture dish in ovarian cancer cells, suggesting that honeycomb films have the possibility of inducing cancer cell to cancer stem cell.