| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
We performed CAGE (Cap Analysis Gene Expression) analysis to investigated transcriptome start sites (TSSs) from the perspective of both the HPV and human genomes. The result showed that the cell polarity and adhesion pathway is dramatically downregulated, and we found a novel antisense viral transcript only seen in CIN cervical cells. Using the molecular and biochemical analyses, we found that cell polarity protein hScrib regulates Ras-ERK pathway, and the activity is mediated in part through recruitment of protein phosphatase 1γ (PP1γ). Furthermore, we have explored the potential role of PP1γ in HPV-induced cervical malignancy. Then, we have found a striking concordance with cellular redistribution in pattern of expression, and this loss of PP1γ expression and redistribution in pattern of expression occurs progressively as the lesions develop. Taken together, we have concluded that PP1γ would be a novel biomarker to predict disease progression for HPV induced malignancy.
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