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Development of the new therapy for cervical cancer and cancer stem cells identification of cancer stem cells

Research Project

Project/Area Number 24592511
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionNagoya University

Principal Investigator

MIZUNO MIKA  名古屋大学, 医学部附属病院, 病院講師 (50588837)

Co-Investigator(Kenkyū-buntansha) SHIBATA Kiyosumi  名古屋大学, 医学系研究科, 准教授 (90335026)
Research Collaborator KAJIYAMA Hiroaki  名古屋大学, 医学系研究科, 准教授 (00345886)
KUMAZAWA Shoko  
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsがん幹細胞 / 放射線治療 / 子宮頸癌 / アポトーシス / 放射線耐性 / 癌幹細胞 / CD133
Outline of Final Research Achievements

Radiotherapy is effective for cervical cancer. However, prior findings indicate that therapeutic failure for these tumors is often associated with the development of radioresistance. We aimed to investigate the possibility of an association between a stem cell-like characters and radiation-resistance in human cervical cancer cells(HeLa). Side-population cells (SP) and non-SP cells (NSP) in HeLa were isolated using flow cytometry with Hoechst 33342 efflux. SP showed a higher expression of cancer stem markers than NSP. SP tended to form spheroids and showed a higher level of tumorigenicity. Furthermore, nude mice performed by subcutaneous injection of SP showed greater tumor growth compared with NSP. SP showed a higher tumorigenicity and lower apoptotic potential than NSP, leading to radiation-tolerance. SP in HeLa showed higher-level stem cell-like properties and radioresistance than NSP. SP might be valuable for new therapeutic approaches for radiation-resistant cervical cancer.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (5 results)

All 2014 2013 2012

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (1 results)

  • [Journal Article] Possible association between stem-like hallmark and radioresistance in human cervical carcinoma cells.2014

    • Author(s)
      Kumazawa S, Kajiyama H, Umezu T, Mizuno M, Suzuki S, Yamamoto E, Mitsui H, Sekiya R, Shibata K, Kikkawa F.
    • Journal Title

      J Obstet Gynaecol Res.

      Volume: 40 Issue: 5 Pages: 1389-98

    • DOI

      10.1111/jog.12357

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High expression of N-acetylglucosaminyltransferase IVa promotes invasion of choriocarcinoma.2013

    • Author(s)
      Niimi K, Yamamoto E, Fujiwara S, Shinjo K, Kotani T, Umezu T, Kajiyama H, Shibata K, Ino K, Kikkawa F.
    • Journal Title

      Br J Cancer

      Volume: 107 Issue: 12 Pages: 1969-1977

    • DOI

      10.1038/bjc.2012.496

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Expression of the potential cancer stem cell markers, CD133, CD44, ALDH1, and beta-catenin, in primary lung adenocarcinoma-their prognostic significance2012

    • Author(s)
      Okudela K, Woo T, Mitsui H, Tajiri M, Masuda M, et al.
    • Journal Title

      Pathol Int

      Volume: 62 Issue: 12 Pages: 792-801

    • DOI

      10.1111/pin.12019

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Functional interaction between peritoneal mesothelial cells and stem cells of ovarian yolk sac tumor (SC-OYST) in peritoneal dissemination.2012

    • Author(s)
      Mitsui H, Shibata K, Suzuki S, Umezu T, Mizuno M, Kajiyama H, Kikkawa F
    • Journal Title

      Gynecol Oncol

      Volume: 124(2) Pages: 303-310

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 子宮頸癌における癌幹細胞の同定と放射線耐性への関与2012

    • Author(s)
      熊澤詔子 水野美香 柴田清住
    • Organizer
      日本産婦人科学会
    • Place of Presentation
      神戸ポートピアホテルおよび神戸国際展示場(神戸)
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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