Elucidation of regulatory mechanism of estrogen in epithelial ovarian cancer

• Project/Area Number: 24592521
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Kumamoto University
• Principal Investigator: MOTOHARA Ken-ichi 熊本大学, 医学部附属病院, 非常勤診療医師 (50573606)
• Co-Investigator(Kenkyū-buntansha): KATABUCHI Hidetaka 熊本大学, 生命科学研究部, 教授 (90224451) ; TASHIRO Hironori 熊本大学, 生命科学研究部, 教授 (70304996) ; MIYAHARA Yo 熊本大学, 生命科学研究部, 非常勤診療医師 (40404355)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 卵巣表層上皮 / 卵巣癌 / 癌幹細胞 / 17β-HSD

Outline of Final Research Achievements

Human ovarian surface epithelium (OSE) is thought to be the origin of most of human ovarian carcinomas. Recent evidence suggests that normal ovarian epithelial stem cells exist in OSE in some mouse models. Here we show that the established immortalized OSE cell lines and the transformed tumorigenic OSE cell lines contain epithelial stem-like cells. Furthermore, RT-PCR analysis demonstrated that these stem-like cell population express various stem cell-related genes. We next plan to check the 17β-HSD expression in these stem-like cell population.

Research Products

• [Journal Article] 婦人科がん領域における放射線療法による副作用の要因解析-皮膚障害に焦点をおいて- (2013)
  • Author(s): 上村 友美、片岡 志歩、松岡 ゆり子、一法師 幸恵、菊池 健、田代 浩徳、髙石 清美、宮原 陽、片渕 秀隆
  • Journal Title: 熊本県母性衛生学会雑誌 Volume: 16 Pages: 35-39
• [Journal Article] 卵巣原発甲状腺腫性カルチノイドの病理組織学的検討：重度の便秘を伴う妊娠女性 (2013)
  • Author(s): 中尾 純子、田代 浩徳、山口 宗影、宮原 陽、本田 律生、大場 隆、大竹 秀幸、猪山 賢一、片渕 秀隆
  • Journal Title: 日本婦人科腫瘍学会雑誌 Volume: 31 Pages: 207-213
  • Peer Reviewed
• [Journal Article] Takeshi Motohara, Hironori Tashiro, Shingo Tayama, Dashdemberel Narantuya, Hidetaka Katabuchi (2013)
  • Author(s): Anti-N-methyl-D-aspartate receptor encephalitis associated with mature cystic teratoma: clinical presentation, diagnosis, treatment, and surgical management
  • Journal Title: Internationa Cancer Conference Jornal Volume: 未定
  • Peer Reviewed
• [Presentation] Epithelial cell adhesion molecule, a potential marker of cancer stem cell, is associated with resistance to platinum-based chemotherapy in epithelial ovarian cancer (2013)
  • Author(s): Tayama S., Motohara T., Katabuchi H.
  • Organizer: The 99th Annual Congress of Korean Society of Obstetrics and Gynecology
  • Place of Presentation: Seoul, Korea
• [Presentation] Epithelial cell adhesion molecule, a potential marker of cancer stem cell, is associated with resistance to platinum-based chemotherapy in epithelial ovarian cancer
  • Author(s): Tayama S., Motohara T., Tjhay F., Narantuya D., Takaishi K., Tashiro T., Katabuchi H.
  • Organizer: The 4th As an-Kumamoto Joint Symposium
  • Place of Presentation: Kumamoto, Japan 阿蘇リゾートグランヴィリオホテル
• [Presentation] Survival impact of wide resection of the pelvic peritoneum in patients with epithelial ovarian cancer：Towards the development of novel surgical strategies for the removal of ovarian cancer stem cells and their niche
  • Author(s): Motohara T., Tayama S., Saito F., Tjhay F., Takaishi K., Sakaguchi I., Tashiro H., Katabuchi K.
  • Organizer: The 4th As an-Kumamoto Joint Symposium
  • Place of Presentation: Kumamoto, Japan 阿蘇リゾートグランヴィリオホテル