analysis of cholesteatoma microbiome in the middle ear

• Project/Area Number: 24592562
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Showa University
• Principal Investigator: Hinohira Yasuyuki 昭和大学, 医学部, 客員教授 (00238320)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 真珠腫 / microbiome / 中耳 / シーケンス / 中耳真珠腫 / マイクロバイオーム / 組織学的検討

Outline of Final Research Achievements

The aim of this study is to compare the difference between otitis media with cholesteatoma patients and those without cholesteatoma on microbial flora of the middle ear mucosa at DNA level. Bacterial samples were collected from 30 cholesteatoma patients and 30 non-cholesteatoma patients. Bacterial-specific 16s rRNA was amplified by PCR and then digested by restriction enzyme. T-RFLP and next generation sequence (Illumina HiSeq) were performed and the data was analyzed. About 20 bacterial species were contained in each sample. Cholesteatoma patients tended to contain more species of anaerobes compared to non-cholesteatoma patients. Anaerobes were considered to get involved with cholesteatoma development.

Research Products

• [Journal Article] 中耳真珠腫に対する外耳道後壁保存段階的鼓室形成術 (2015)
  • Author(s): 比野平恭之
  • Journal Title: 耳鼻咽喉科臨床 Volume: 108 Pages: 426-427
  • NAID: 130005072181
  • Peer Reviewed
• [Journal Article] 進展度分類に応じた真珠腫に対する鼓室形成術． (2014)
  • Author(s): 比野平恭之
  • Journal Title: Otol Jpn Volume: 24 Pages: 801-804
  • NAID: 130005166001
  • Peer Reviewed
• [Presentation] 鼓室形成術習熟への取り組み (2015)
  • Author(s): 比野平恭之
  • Organizer: 第25回日本耳科学会総会学術講演会
  • Place of Presentation: 長崎
  • Year and Date: 2015-10-07
  • Invited
• [Presentation] 中耳真珠腫に対する後壁保存段階的鼓室形成術． (2014)
  • Author(s): 比野平恭之，小林一女，鈴木貴裕．
  • Organizer: 第115回日本耳鼻咽喉科学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2014-10-16 – 2014-10-18
• [Presentation] Postoperative management of residual cholesteatoma and less invasive revision surgery for the recurrence. (2013)
  • Author(s): Hinohira Y, Matsuda K, Kobayashi H, Tono T, Suzaki H, Yanagihara Y.
  • Organizer: The 29th Politzer society meeting
  • Place of Presentation: Antalya, Turkey
• [Presentation] Intratympanic membrane cholesteatoma in two pediatric cases. (2013)
  • Author(s): Ishibashi A, Hinohira Y, Takahashi G, Kobayashi H, Suzaki H.
  • Organizer: The 29th Politzer society meeting
  • Place of Presentation: Antalya, Turkey
• [Presentation] 進展度分類に応じた真珠腫に対する鼓室形成術. (2013)
  • Author(s): 比野平恭之．
  • Organizer: 第23回日本耳科学会総会
  • Place of Presentation: 宮崎
• [Presentation] 進展度分類に応じた真珠腫に対する鼓室形成術 (2012)
  • Author(s): 比野平恭之
  • Organizer: 第22回日本耳科学会
  • Place of Presentation: 名古屋