Methylation analysis by using next generation DNA sequencer in HNSCC.

• Project/Area Number: 24592594
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: MINETA Hiroyuki 浜松医科大学, 医学部, 教授 (40190714)
• Co-Investigator(Kenkyū-buntansha): MISAWA Kiyoshi 浜松医科大学, 医学部附属病院, 講師 (90334979)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ゲノム網羅的メチル化解析 / 頭頸部癌 / 中咽頭癌 / HPV / 次世代シークエンサー / 網羅的メチル化解析 / ＨＰＶ / 5-Azacytidine

Outline of Final Research Achievements

In the present study, we prospectively analyzed HPV realted oropharyngeal cancer and normal side tonsil by using next generation DNA sequencer.
We analyzed the promoter methylation of 3 genes (MAL, SLC5A5, NUP85) in 133 HNSCC and in matching 42 normal tissues.

Research Products

• [Journal Article] Tumor suppressor activity and inactivation of galanin receptor type 2 by aberrant promoter methylation in head and neck cancer (2014)
  • Author(s): Misawa Y, Misawa K, Kanazawa T, Uehara T, Endo S, Mochizuki D, Yamatodani T, Carey TE, Mineta H
  • Journal Title: Cancer Volume: 120 Issue: 2 Pages: 205-213
  • DOI: 10.1002/cncr.28411
  • Peer Reviewed / Open Access
• [Journal Article] Novel anti-tumor mechanism of galanin receptor type 2 in head and neck squamous cell carcinoma cells (2014)
  • Author(s): Uehara T, Kanazawa T, Mizukami H, Uchibori R, Tsukahara T, Urabe M, Kume A, Misawa K, Carey T, Suzuki M, Ichimura K, Ozawa K
  • Journal Title: Cancer Science Volume: 105 Issue: 1 Pages: 72-80
  • DOI: 10.1111/cas.12315
  • Peer Reviewed / Open Access
• [Journal Article] Prognostic value of Type XXII and XXIV collagen mRNA expression in head and neck cancer patients (2014)
  • Author(s): Misawa K, Kanazawa T, Imai A, Endo S, Mochizuki D, Fukushima H, Misawa Y, Mineta H
  • Journal Title: Molecular and Clinical Oncology Volume: 2 Pages: 285-291
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Frequent promoter hypermethylation of tachykinin-1 and tachykinin receptor type 1 is a potential biomarker for head and neck cancer. (2013)
  • Author(s): Misawa K*, Kanazawa T, Misawa Y, Imai A, Uehara T, Mochizuki D, Endo S, Takahashi G, Mineta H
  • Journal Title: J Cancer Res Clin Volume: 100 Issue: 5 Pages: 879-889
  • DOI: 10.1007/s00432-013-1393-5
  • Peer Reviewed
• [Journal Article] Galanin has a Tumor Suppressor Activity and is Frequently Inactivated by Aberrant Promoter Methylation in Head and Neck Cancer. (2013)
  • Author(s): Misawa K, Kanazawa T, Misawa Y, Uehara T, Imai A, Takahashi G, Takebayashi S, Cole A, Carey TE, Mineta H
  • Journal Title: Transl Oncol Volume: 6(3) Pages: 338-346
  • Peer Reviewed
• [Journal Article] Rare case of malignant transformation of recurrent respiratory papillomatosis associated with human papillomavirus type 6 infection and p53 overexpression. (2013)
  • Author(s): Kanazawa T, Fukushima N, Imayoshi S, Nagatomo T, Kawada K, Nishino H, Misawa K, Ichimura K
  • Journal Title: SpringerPlus Volume: 2(1) Pages: 153-153
  • Peer Reviewed
• [Journal Article] 浜松医科大学における中咽頭癌の臨床統計 (2013)
  • Author(s): 三澤清、遠藤志織、望月大極、伴昭宏、杉山健一、岡村純、瀧澤義徳、高橋吾郎、大和谷崇、細川誠二、峯田周幸
  • Journal Title: 耳鼻咽喉科臨床 補刷 Volume: 136 Pages: 18-20
  • NAID: 10031188255
  • Peer Reviewed
• [Journal Article] Hypermethylation of Collagen α2(I) Gene (COL1A2) is a Potential Biomarker in Head and Neck Cancer. (2012)
  • Author(s): Misawa K*, Kanazawa T, Misawa Y, Imai A, Endo S, Hakamada K, Mineta H
  • Journal Title: Cancer Biomarkers Volume: 10 Issue: 3-4 Pages: 135-144
  • DOI: 10.3233/cbm-2012-0242
  • Peer Reviewed
• [Presentation] 頭頸部癌におけるエピジェネティック分子機構について パネルディスカッション (2015)
  • Author(s): 三澤 清、峯田周幸
  • Organizer: 第25回日本気管食道科学会認定専門医大会
  • Place of Presentation: 宇都宮
  • Year and Date: 2015-02-21 – 2015-02-22
  • Invited
• [Presentation] 中咽頭癌症例のHPV解析とDNAメチル化解析 (2014)
  • Author(s): 三澤 清、新井宏幸、石川竜司、岡村純、瀧澤義徳、三澤由幾、袴田桂、金澤丈治、峯田周幸
  • Organizer: 第38回日本頭頸部癌学会
  • Place of Presentation: 東京
  • Year and Date: 2014-06-12 – 2014-06-13
• [Presentation] 頭頸部癌におけるオレキシン遺伝子の発現とプロモーター領域のメチル化との関連について (2014)
  • Author(s): 三澤 清、今井篤志、遠藤志織、望月大極、三澤由幾、高橋吾郎、金澤丈治、峯田周幸
  • Organizer: 第115回日本耳鼻咽喉科学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2014-05-15 – 2014-05-17
• [Presentation] Expression changes in BMI-1 and EZH2 are associated with DNA methylation changes in Head and Neck cancer (2013)
  • Author(s): Kiyoshi Misawa、Mineta Hiroyuki
  • Organizer: 20th IFOS World Congress
  • Place of Presentation: Seoul
• [Presentation] 頭頸部癌におけるソマトスタチン遺伝子の発現とプロモーター領域のメチル化との関連について (2013)
  • Author(s): 三澤 清、金澤丈治、今井篤志、三澤由幾、上原貴行、遠藤志織、望月大極、杉山健一、高橋吾郎、峯田周幸
  • Organizer: 第114回日本耳鼻咽喉科学会総会
  • Place of Presentation: 札幌
• [Presentation] 頭頸部癌における臨床検体の腫瘍部と正常部のDNAメチル化解析について (2013)
  • Author(s): 三澤 清、高橋吾郎、三澤由幾、杉山健一、瀧澤義徳、今井篤志、金澤丈治、峯田周幸
  • Organizer: 第37回日本頭頸部癌学会
  • Place of Presentation: 東京
• [Presentation] Hypermethylation of tachykinin-1 and tachykinin receptor type 1 is a potential biomarker for head and neck cancer (2013)
  • Author(s): 三澤 清、金澤丈治、峯田周幸
  • Organizer: 第72回日本癌学会
  • Place of Presentation: 横浜
• [Presentation] 頭頸部癌におけるポリコーム群複合体遺伝子の発現と癌抑制遺伝子のメチル化の関連とその予後について (2012)
  • Author(s): 三澤 清、金澤丈治、今井篤志、杉山健一、瀧澤義徳、高橋吾郎、細川誠二、峯田周幸
  • Organizer: 第113回日本耳鼻咽喉科学会総会
  • Place of Presentation: 新潟
• [Presentation] 頭頸部癌におけるGALR2遺伝子の発現解析およびDNAメチル化解析と予後について (2012)
  • Author(s): 三澤 清、金澤丈治、今井篤志、杉山健一、瀧澤義徳、高橋吾郎、細川誠二、峯田周幸
  • Organizer: 第36回日本頭頸部癌学会
  • Place of Presentation: 松江
• [Presentation] Galanin and Galanin receptors are Frequently Inactivated by Aberrant Promoter Methylation in Head and Neck Cancer. (2012)
  • Author(s): 三澤 清、金澤丈治、峯田周幸
  • Organizer: 第71回日本癌学会
  • Place of Presentation: 札幌