| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Vascular adhesion protein (VAP)-1, a multifunctional molecule with adhesive and enzymatic properties, is expressed at the surface of vascular endothelial cells of mammals. It also exists as a soluble form (sVAP-1), which is implicated in oxidative stress via its enzymatic activity. This study explores a mechanistic components to form sVAP-1 in retinal endothelial cells. Retinal capillary endothelial cells released sVAP-1 when stimulated with high glucose or inflammatory cytokines such as tumor necrosis factor-α , interleukin-1β and vascular endothelial growth factor in vitro. Furthermore, matrix metalloproteinase -2 and -9, type IV collagenases, were the key molecules to mediate the protein cleavage of VAP-1 from retinal capillary endothelial cells. Our data for the first time provide the evidence on the sVAP-1 production mediated by inflammatory cytokines and type IV collagenases in the pathogenesis of diabetic retinopathy.
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