Production of soluble vascular adhesion protein-1 in diabetic retinopathy
| Project/Area Number |
24592615
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Hokkaido University |
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Principal Investigator |
NODA KOUSUKE 北海道大学, 医学(系)研究科(研究院), 准教授 (90296666)
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| Co-Investigator(Kenkyū-buntansha) |
KANDA Atsuhiro 北海道大学, 大学院医学研究科, 特任講師 (80342707)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 糖尿病網膜症 / 白血球接着分子 / 酸化ストレス / VAP-1 / MMPs |
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| Outline of Final Research Achievements |
Vascular adhesion protein (VAP)-1, a multifunctional molecule with adhesive and enzymatic properties, is expressed at the surface of vascular endothelial cells of mammals. It also exists as a soluble form (sVAP-1), which is implicated in oxidative stress via its enzymatic activity. This study explores a mechanistic components to form sVAP-1 in retinal endothelial cells. Retinal capillary endothelial cells released sVAP-1 when stimulated with high glucose or inflammatory cytokines such as tumor necrosis factor-α , interleukin-1β and vascular endothelial growth factor in vitro. Furthermore, matrix metalloproteinase -2 and -9, type IV collagenases, were the key molecules to mediate the protein cleavage of VAP-1 from retinal capillary endothelial cells. Our data for the first time provide the evidence on the sVAP-1 production mediated by inflammatory cytokines and type IV collagenases in the pathogenesis of diabetic retinopathy.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Soluble vascular adhesion protein-1 accumulates in proliferative diabetic retinopathy2012
Author(s)
Murata M, Noda K, Fukuhara J, Kanda A, Kase S, Saito W, Ozawa Y, Mochizuki S, Kimura S, Mashima Y, Okada Y, Ishida S.
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Journal Title
Invest Ophthalmol Vis Sci
Volume: 53
Pages: 4055-4062
Related Report
Peer Reviewed
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