Pathological mechanism and a new method for the treatment of retinal vein occlusion using sugar-chain modified liposome.

• Project/Area Number: 24592623
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Shiga University of Medical Science
• Principal Investigator: SAISHIN YOSHITSUGU 滋賀医科大学, 医学部, 講師 (30379193)
• Co-Investigator(Kenkyū-buntansha): OHJI Masahito 滋賀医科大学, 医学部, 教授 (90252650)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: タンパク質 / 細胞・組織 / 応用動物 / 脂質 / 蛋白質

Outline of Final Research Achievements

Liposome is one of the most effective tools for drug delivery systems. Liposome offers improved pharmacokinetic properties, controlled and sustained release of drugs. liposomes encapsulated with human immunoglobulin for intravitreal administration were prepared and characterized. The liposomes were prepared using the improved cholate dialysis method. The intensity-average diameter of the liposomal nanoparticles was approximately 100 nm. Encapsulation efficiency was approximately 16-21%. The size distribution of liposomes remained stable until 45 days. The efficiency of leakage at 60 days was approximately 35 %. The leakage concentration of human immunoglobulin was gradually increased during the study period. The liposomes can lead to the slow and controlled release of drug in the vitreous. These results suggest that liposomes encapsulated with immunoglobulin for intravitreal administration may be feasible for antibody treatment of ocular diseases.

Research Products

• [Journal Article] Comparison of single injection and three monthly injections of intravitreal bevacizumab for macular edema associated with branch retinal vein occlusion. (2015)
  • Author(s): Ito Y, Saishin Y, Sawada O, Kakinoki M, Miyake T, Sawada T, Kawamura H, Ohji M
  • Journal Title: Clinical ophthalmology Volume: 9 Pages: 175-180
  • DOI: 10.2147/opth.s76261
  • NAID: 120006546341
  • Peer Reviewed
• [Journal Article] Anterior chamber paracentesis might prevent sustained intraocular pressure elevation after intravitreal injections of ranibizumab for age-related macular degeneration. (2014)
  • Author(s): Ichiyama Y, Sawada T, Kakinoki M, Sawada O, Nakashima T, Saishin Y, Kawamura H, Ohji M
  • Journal Title: Ophthalmic research Volume: 52 Issue: 4 Pages: 234-238
  • DOI: 10.1159/000365253
  • Peer Reviewed
• [Journal Article] Scleral imbrication combined with vitrectomy and gas tamponade for refractory macular hole retinal detachment associated with high myopia. (2014)
  • Author(s): Fujikawa M, Kawamura H, Kakinoki M, Sawada O, Sawada T, Saishin Y, Ohji M
  • Journal Title: Retina Volume: 34 Issue: 12 Pages: 2451-2457
  • DOI: 10.1097/iae.0000000000000246
  • Peer Reviewed
• [Journal Article] Serum and plasma vascular endothelial growth factor concentrations before and after intravitreal injection of aflibercept or ranibizumab for age-related macular degeneration. (2014)
  • Author(s): Wang X, Sawada T, Sawada O, Saishin Y, Liu P, Ohji M
  • Journal Title: American journal of ophthalmology Volume: 158 Issue: 4 Pages: 738-744
  • DOI: 10.1016/j.ajo.2014.06.009
  • Peer Reviewed
• [Journal Article] Bimonthly injections of ranibizumab for age-related macular degeneration. (2014)
  • Author(s): Sawada T, Kakinoki M, Wang X, Kawamura H, Saishin Y, Ohji M
  • Journal Title: Graefes Arch Clin Exp Ophthalmol Volume: 252 Issue: 10 Pages: 1545-1551
  • DOI: 10.1007/s00417-014-2612-8
  • Peer Reviewed
• [Journal Article] Aqueous vascular endothelial growth factor and ranibizumab concentrations after monthly and bimonthly intravitreal injections of ranibizumab for age-related macular degeneration. (2013)
  • Author(s): Wang X, Sawada T, Kakinoki M, Miyake T, Kawamura H, Saishin Y, Liu P, Ohji M
  • Journal Title: Graefes Arch Clin Exp Ophthalmol. Volume: Nov 7.
  • Peer Reviewed
• [Journal Article] Effect of vitrectomy on aqueous VEGF concentration and pharmacokinetics of bevacizumab in macaque monkeys. (2012)
  • Author(s): Kakinoki M, Sawada O, Sawada T, Saishin Y, Kawamura H, Ohji M.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 53 Issue: 9 Pages: 5877-5880
  • DOI: 10.1167/iovs.12-10164
  • Peer Reviewed
• [Journal Article] Prevalence of sleep-disordered breathing in patients with retinal vein occlusion. (2012)
  • Author(s): Kanai H, Shiba T, Hori Y, Saishin Y, Maeno T, Takahashi M.
  • Journal Title: Nihon Ganka Gakkai Zasshi. Volume: 116 Pages: 81-85
  • NAID: 10030249072
  • Peer Reviewed
• [Presentation] Characterization and evaluation of sugar-chain modified liposome encapsulated with human immunoglobulin for intravitreal administration (2014)
  • Author(s): Iwasa M, Saishin Y, Wang X, Ohji M
  • Organizer: Annual Meeting of Association for Research in Vision and Ophthalmology, 2014
  • Place of Presentation: ORLANDO, FL,U.S.A
  • Year and Date: 2014-05-04 – 2014-05-08
• [Presentation] Bimonthly injections of ranibizumab for age-related macular degeneration (2014)
  • Author(s): Sawada T, Wang X, Sawada O, Saishin Y, Ohji M
  • Organizer: Annual Meeting of Association for Research in Vision and Ophthalmology, 2014
  • Place of Presentation: ORLANDO, FL,U.S.A
  • Year and Date: 2014-05-04 – 2014-05-08
• [Presentation] Serum and aqueous vascular endothelial growth factor concentration of patients with age-related macular degeneration after intravitreal injection of aflibercept (2014)
  • Author(s): Wang X, Sawada T, Sawada O, Saishin Y, Ohji M
  • Organizer: Annual Meeting of Association for Research in Vision and Ophthalmology, 2014
  • Place of Presentation: ORLANDO, FL,U.S.A
  • Year and Date: 2014-05-04 – 2014-05-08
• [Presentation] Photoreceptor outer segment length and outer foveal thickness as predictive factors associated with visual outcome after vitrectomy for vitreomacular traction syndrome (2014)
  • Author(s): Ichiyama Y, Kawamura H, Fujikawa M, Sawada O, Saishin Y, Ohji M
  • Organizer: Annual Meeting of Association for Research in Vision and Ophthalmology, 2014
  • Place of Presentation: ORLANDO, FL,U.S.A
  • Year and Date: 2014-05-04 – 2014-05-08
• [Presentation] Changes of retinal thickness after vitrectomy for epiretinal membrane with and without internal limiting membrane peeling (2014)
  • Author(s): Obata S, Iwasaki K, Fujikawa M, Kakinoki M, Sawada O, Saishin Y, Kawamura H, Ohji M
  • Organizer: Annual Meeting of Association for Research in Vision and Ophthalmology, 2014
  • Place of Presentation: ORLANDO, FL,U.S.A
  • Year and Date: 2014-05-04 – 2014-05-08
• [Presentation] Two-year efficacy of six monthly injections of intravitreal bevacizumab for macular edema associated with central retinal vein occlusion. (2013)
  • Author(s): Saishin Y, Sawada O, Fujikawa M, Sawada T, Kawamura H, Ohji M
  • Organizer: The 8th Asia-Pacific Vitreo-retinal Society Congress 2013
  • Place of Presentation: Nagoya, Japan
• [Presentation] Aqueous Vascular Endothelial Growth Factor and Ranibizumab Concentrations after Monthly and Bimonthly Intravitreal Injections of Ranibizumab for Age-Related Macular Degeneration. (2013)
  • Author(s): Wang X, Sawada T, Kakinoki M, Miyake T, Kawamura H, Saishin Y, Liu P, Ohji M
  • Organizer: Annual Meeting of Association for Research in Vision and Ophthalmology, 2013
  • Place of Presentation: Ｓｅａｔｔｌｅ, USA
• [Presentation] The efficacy of bimonthly injection of ranibizumab for age-related macular degeneration for six months. (2013)
  • Author(s): Sawada T, Kakinoki M, Wang X, Kawamura H, Saishin Y, Ohji M
  • Organizer: Annual Meeting of Association for Research in Vision and Ophthalmology, 2013
  • Place of Presentation: Seattle, USA
• [Presentation] A lack of delayed intraocular pressure elevation after intravitreal injections of ranibizumab for age-related macular degeneration. (2013)
  • Author(s): Ichiyama Y, Sawada T, Kakinoki M, Sawada O, Saishin Y, Kawamura H, Ohji M
  • Organizer: Annual Meeting of Association for Research in Vision and Ophthalmology, 2013
  • Place of Presentation: Seattle, USA
• [Presentation] Intravitreal Injections of Bevacizumab in Patients with Macular Edema Following Branch Retinal Vein Occlusion; 1-Year Outcomes (2012)
  • Author(s): Yuka Ito, Yoshitsugu Saishin, Osamu Sawada, Masashi Kakinoki, Taichiro Miyake, Tomoko Sawada, Hajime Kawamura, Masahito Ohji
  • Organizer: The 27th Asia Pacific Academy of Ophthalmology Congress
  • Place of Presentation: Busan, Korea