| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Genotyping of single-nucleotide polymorphisms in candidate genes for the development of intraocular inflammation such as cytokines, adhesion molecules, and antioxidant enzymes genes was performed using polymerase chain reaction-restriction fragment length polymorphism analysis. There was no significant association between any of the single-nucleotide polymorphisms analyzed and intraocular inflammation. For the purpose of delivering genes controlling the factors involved in the development of intraocular inflammation selectively into the eye, we performed gene transfer to polarized retinal pigment epithelium cells by ultrasound irradiation with combination of a bubble liposome, and confirmed gene delivery to the polarized retinal pigment epithelium cells with high efficiency.
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