| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Retinal pigment epithelial (RPE) cells form a blood retinal barrier, and their polarized property is crucial for maintaining the barrier functions. Cell culture RPE models have contributed to our understanding of how RPE cells are involved in the pathophysiology of retinochoroidal diseases, however, most studies have been performed on weakly polarized cells, such as ARPE-19 cells, which do not have barrier properties. Recently, a new cell culture method was developed that produced RPE cells with characteristics of the native tissue which called polarized RPE. Exposure to Tumor Necrosis Factor-a decreased the vascular endothelial growth factor secretion in polarized RPE cells but increased it in non- polarized RPE cells in culture. Cell polarization is an important factor for determining the response of RPE cells to various cytokines, and the different responsive patterns to these upon cell polarity might explain the complicated pathology of corio-retinal diseases.
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