The difference of cell reactivity from the presence or absence of retinal pigment epithelial cell polarity
Project/Area Number |
24592634
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Ophthalmology
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Research Institution | Kagoshima University |
Principal Investigator |
SONODA Shozo 鹿児島大学, 医学部・歯学部病院, 講師 (20325806)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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Keywords | 細胞極性 / 網膜色素上皮細胞 / 細胞培養 / 腫瘍壊死因子 / 細胞間接着装置 / 極性細胞 / 極性 / TNF-a / トロンビン / 糖尿病黄斑症 / VEGF / 網膜色素上皮 |
Outline of Final Research Achievements |
Retinal pigment epithelial (RPE) cells form a blood retinal barrier, and their polarized property is crucial for maintaining the barrier functions. Cell culture RPE models have contributed to our understanding of how RPE cells are involved in the pathophysiology of retinochoroidal diseases, however, most studies have been performed on weakly polarized cells, such as ARPE-19 cells, which do not have barrier properties. Recently, a new cell culture method was developed that produced RPE cells with characteristics of the native tissue which called polarized RPE. Exposure to Tumor Necrosis Factor-a decreased the vascular endothelial growth factor secretion in polarized RPE cells but increased it in non- polarized RPE cells in culture. Cell polarization is an important factor for determining the response of RPE cells to various cytokines, and the different responsive patterns to these upon cell polarity might explain the complicated pathology of corio-retinal diseases.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Dynamic increase in extracellular ATP accelerates photoreceptor cell apoptosis via ligation of P2RX7 in subretinal hemorrhage2013
Author(s)
Notomi S, Hisatomi T, Murakami Y, Terasaki H, Sonoda S, Asato R, Takeda A, Ikeda Y, Enaida H, Sakamoto T, Ishibashi T
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Journal Title
PLoS One
Volume: epub ahead
Issue: 1
Pages: e53338-e53338
DOI
Related Report
Peer Reviewed
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