| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The identification of neural crest stem cells in various tissues opens an entirely new approach to developing autologous cell replacement therapies for use in regenerative medicine. Recently, skin derived precursors (SKPs) were isolated and characterized as a potent precursor cell population from the adult mammalian dermis. In this study, we succeeded in direct differentiation of murine facial SKPs into functional tissue engineered corneal endothelium (TECE). In vitro analysis of pump function by Ussing chamber and in vivo transplantation into a corneal endothelial dysfunction rabbit model, SKP-induced TECE contributed in recovering corneal transparency. Furthermore, we succeeded in isolating and expanding human SKPs from eldery subjects. However, some other factors may be required to induce human SKPs into corneal endothelium. Overall, our findings demonstrate that facial SKPs are an attractive autologous source of progenitor cells for corneal endothelial regeneration.
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