Analysis of limbal epithelial tiny cells contact with limbal niche like cells
Project/Area Number |
24592646
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Ophthalmology
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Research Institution | Tokyo Dental College (2013-2014) Keio University (2012) |
Principal Investigator |
HIGA Kazunari 東京歯科大学, 歯学部, 助教 (60398782)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 角膜上皮幹細胞 |
Outline of Final Research Achievements |
Stem cells have a specialized microenvironment for maintaining self-renewal and multipotent capacities. It is believed that a cornea epithelial stem cell niche exists in the limbus. We presented that aquaporin 1 positive (AQP1+) cells immediately beneath epithelial basement membrane may be mesenchymal niche cells that directly interact with N-cadherin positive (N-cad+) limbal basal epithelial cells. To establish a cultivation method the limbal epithelial stem cells niche in vitro, we performed spheroidal cultivation of Tiny cells from the human limbus using Matirgel. Keratin (K) 15 and p63 were expressed in the spheroid after 1-month cultivation, as well as in epithelial sheets engineered form a single spheroid. K15 and p63 were expressed in the edge of spheroids after 1-month cultivation under hypoxia. In addition, N-cadherin was expressed in epithelial spheroid under hypoxia.
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Report
(4 results)
Research Products
(5 results)
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[Presentation] 角膜輪部ニッチ培養法の検討2014
Author(s)
比嘉一成、宮下英之、島﨑潤、坪田一男、榛村重人
Organizer
第35回日本炎症・再生医学会
Place of Presentation
沖縄
Year and Date
2014-07-01 – 2014-07-04
Related Report
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