The use of induced pluripotent stem cells to reveal pathogenic gene mutations and explore treatments for retinitis pigmentosa
Project/Area Number |
24592647
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Ophthalmology
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Research Institution | Keio University |
Principal Investigator |
OZAWA Yoko 慶應義塾大学, 医学部, 講師 (90265885)
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Research Collaborator |
TETSU Yoshida 慶應義塾大学, 医学部, 特任助教
WADO Akamatsu 慶應義塾大学, 医学部, 講師 (60338184)
HARUNA Mabuchi 慶應義塾大学, 医学部, 特任研究員 (70465011)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 網膜 / 幹細胞 / iPS / 再生 / 神経保護 |
Outline of Final Research Achievements |
We generated induced pluripotent stem cells (iPSCs) from an RP patient carrying a rhodopsin mutation (E181K). The cells were then subjected to retinal differentiation; the resulting rod photoreceptor cells were labeled with an Nrl promoter-driven enhanced EGFP-carrying adenovirus and purified using flow cytometry after 5 weeks of culture. Using this approach, we found a reduced survival rate in the photoreceptor cells with the E181K mutation, which was correlated with the increased expression of endoplasmic reticulum (ER) stress and apoptotic markers. The use of RP patient-derived iPSCs provided a system that can be used to explore candidate therapeutic approaches.
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Report
(4 results)
Research Products
(65 results)
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[Journal Article] Clinical and Molecular Characteristics of Childhood-onset Stargardt Disease.2015
Author(s)
Fujinami K, Jana Zernant, Ravinder K Chana, Genevieve A Wright, Tsunoda K, Ozawa Y, Tsubota K, Anthony G Robson, Graham E Holder, Rando Allikmets, Michel Michaelides, FACS; Anthony T Moore.
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Journal Title
Ophthalmology.
Volume: 122
Issue: 2
Pages: 326-334
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Association of Maternal Age to Development and Progression of Retinopathy of Prematurity in Infants of Gestational Age under 33 Weeks.2014
Author(s)
Uchida A, Miwa M, Shinoda H, Koto T, Nagai N, Mochimaru H, Tomita Y, Sasaki M, Ikeda K, Tsubota K, Ozawa Y
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Journal Title
J Ophthalmol.
Volume: 2014
Pages: 187929-187929
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] The use of induced pluripotent stem cells to reveal pathogenic gene mutations and explore treatments for retinitis pigmentosa.2014
Author(s)
Yoshida T, Ozawa Y, Suzuki K, Yuki K, Ohyama M, Akamatsu W, Matsuzaki Y, Shimmura S, Mitani K, Tsubota K, Okano H.
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Journal Title
Mol Brain.
Volume: 7
Issue: 1
Pages: 45-45
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Decreased proteasomal activity causes photoreceptor degeneration in mice.2014
Author(s)
Ando R, Noda K, Tomaru U, Kamoshita M, Ozawa Y, Notomi S, Hisatomi T, Noda M, Kanda A, Ishibashi T, Kasahara M, Ishida S.
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Journal Title
Invest Ophthalmol Vis Sci.
Volume: 55
Issue: 7
Pages: 4682-4690
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The Association between Visual Field Defect Severity and Fear of Falling in Primary Open-Angle Glaucoma.2013
Author(s)
Yuki K, Tanabe S, Kouyama K, Fukagawa K, Uchino M, Shimoyama M, Ozeki N, Shiba D, Ozawa Y, Abe T, Tsubota K.
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Journal Title
Invest Ophthalmol Vis Sci.
Volume: 54
Issue: 12
Pages: 7739-7745
DOI
Related Report
Peer Reviewed
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[Journal Article] Angiotensin II type 1 receptor antagonist attenuates lacrimal gland, lung, and liver fibrosis in a murine model of chronic graft-versus-host disease.2013
Author(s)
Yaguchi S, Ogawa Y, Shimmura S, Kawakita T, Hatou S, Satofuka S, Nakamura S, Imada T, Miyashita H, Yoshida S, Yaguchi T, Ozawa Y, Mori T, Okamoto S, Kawakami Y, Ishida S, Tsubota K.
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Journal Title
PLoS ONE
Volume: 8
Issue: 6
Pages: e64724-e64724
DOI
Related Report
Peer Reviewed
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[Journal Article] Molecular Characteristics of Four Japanese Cases with KCNV2-retinopathy: Report of Novel Disease-causing Variants.2013
Author(s)
Fujinami K, Tsunoda K, Nakamura N, Kato Y, Noda T, Shinoda K, Tomita K, Hatase T, Usui T, Akahori M, Itabashi T, Iwata T, Ozawa Y, Tsubota K, Miyake Y.
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Journal Title
Molecular Vision.
Volume: 19
Pages: 1580-1590
Related Report
Peer Reviewed
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[Journal Article] Presence and physiological function of Renin-Angiotensin System in mouse lacrimal gland.2012
Author(s)
Yaguchi S, Ogawa Y, Shimmura S, Hatou S, Nakamura S, Inaba T, Imada T, Ozawa Y, Kawakami Y, Ishida S, Tsubota K.
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Journal Title
Invest Ophthalmol Vis Sci.
Volume: 53
Issue: 9
Pages: 5416-25
DOI
Related Report
Peer Reviewed
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[Journal Article] Soluble Vascular Adhesion Protein-1 Accumulates in Proliferative Diabetic Retinopathy.2012
Author(s)
Murata M, Noda K, Fukuhara J, Kanda A, Kase S, Saito W, Ozawa Y, Mochizuki S, Kimura S, Mashima Y, Okada Y, Ishida S.
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Journal Title
Invest Ophthalmol Vis Sci.
Volume: 53
Issue: 7
Pages: 4055-62
DOI
Related Report
Peer Reviewed
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[Journal Article] Acute visual field defect following vitrectomy determined to originate from optic nerve by electrophysiological tests.2012
Author(s)
Uchida A, Shinoda K, Matsumoto C.S, Kawai M, Kawai S, Ohde H, Ozawa Y, Ishida S, Inoue M, Mizota A, Tsubota K.
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Journal Title
Case Rep Ophthalmol.
Volume: 3
Issue: 3
Pages: 396-405
DOI
Related Report
Peer Reviewed
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[Presentation] Associations of Serum Lipids with Macular Morphology in Patients without Diabetic Macular Edema.2013
Author(s)
Sasaki M, Kawashima M, Kawasaki R, Kawai M, Uchida A, Koto T, Mochimaru H, Shinoda H, Jie Jin Wang, Tsubota K, Ozawa Y.
Organizer
Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting
Place of Presentation
Washington State Convention & Trade Center Seattle
Related Report
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[Presentation] 狭義加齢黄斑変性に対するpro re nateによるラニビズマブ硝子体注射.2013
Author(s)
鈴木美砂, 永井紀博, 永井香奈子, 篠田肇, 内田敦郎, 厚東隆志, 持丸博史, 富田洋平, 佐々木真理子, 坪田一男, 小沢洋子.
Organizer
第67回日本臨床眼科学会
Place of Presentation
パシフィコ横浜 (神奈川県横浜市)
Related Report
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[Presentation] An analysis of the mechanisms of degradation of photoreceptor cells in a retinitis pigmentosa patient using iPS cells.2012
Author(s)
Yoshida T, Ozawa Y, Hirabayashi Y, Suzuki K, Mitani K, Kobayashi T, Ohyama M, Amagai M, Okada Y, Akamatsu W, Tsubota K, Shimmura S, Okano H.
Organizer
International Society for Stem Cell Research (ISSCR) 10th annual meeting,
Place of Presentation
神奈川県横浜市
Related Report
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[Presentation] An analysis of the mechanisms of degradation of photoreceptor cells in a retinitis pigmentosa patient using iPS cells.2012
Author(s)
Yoshida T, Ozawa Y, Suzuki K, Hirabayashi Y, Suzuki S, Koizumi H, Yuki K, Kobayashi T, Ohyama M, Amagai M, Okada Y, Akamatsu W, Matsuzaki Y, Mitani K, Shimmra S, Tsubota K, Okano H.
Organizer
Global COE Final Symposium .Center for Integrated Medical Research,
Place of Presentation
東京都新宿区
Related Report