The use of induced pluripotent stem cells to reveal pathogenic gene mutations and explore treatments for retinitis pigmentosa

• Project/Area Number: 24592647
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Keio University
• Principal Investigator: OZAWA Yoko 慶應義塾大学, 医学部, 講師 (90265885)
• Research Collaborator: TETSU Yoshida 慶應義塾大学, 医学部, 特任助教 ; WADO Akamatsu 慶應義塾大学, 医学部, 講師 (60338184) ; HARUNA Mabuchi 慶應義塾大学, 医学部, 特任研究員 (70465011)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 網膜 / 幹細胞 / iPS / 再生 / 神経保護

Outline of Final Research Achievements

We generated induced pluripotent stem cells (iPSCs) from an RP patient carrying a rhodopsin mutation (E181K). The cells were then subjected to retinal differentiation; the resulting rod photoreceptor cells were labeled with an Nrl promoter-driven enhanced EGFP-carrying adenovirus and purified using flow cytometry after 5 weeks of culture. Using this approach, we found a reduced survival rate in the photoreceptor cells with the E181K mutation, which was correlated with the increased expression of endoplasmic reticulum (ER) stress and apoptotic markers. The use of RP patient-derived iPSCs provided a system that can be used to explore candidate therapeutic approaches.

Research Products

• [Journal Article] Circadian control of p75 neurotrophin receptor leads to alternate activation of Nrf2 and c-Rel to reset energy metabolism in astrocytes via brain-derived neurotrophic factor. (2018)
  • Author(s): 新美和寛
  • Journal Title: Free Radic Biol Me Volume: 52 Pages: 1-10
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Clinical and Molecular Characteristics of Childhood-Onset Stargardt Disease. (2015)
  • Author(s): Fujinami K, Jana Zernant, Ravinder K Chana, Genevieve A Wright, Tsunoda K, Ozawa Y, Tsubota K, Anthony G Robson, Graham E Holder, Rando Allikmets, Michel Michaelides, FACS; Anthony T Moore.
  • Journal Title: Ophthalmology Volume: 122 Pages: 326-334
  • DOI: 10.1016/j.ophtha.2014.08.012
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Wickerhamomyces anomalus Fungal Keratitis Responds to Topical Treatment with Antifungal Micafungin. (2015)
  • Author(s): Kamoshita M, Matsumoto Y, Nishimura K, Katono Y, Murata M, Ozawa Y, Shimmura S, Tsubota K.
  • Journal Title: Journal of Infection and Chemotherapy. Volume: 21 Pages: 141-143
  • DOI: 10.1016/j.jiac.2014.08.019
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Phase II enzyme induction by a carotenoid, lutein, in a PC12D neuronal cell line. (2014)
  • Author(s): Miyake S, Kobayashi S, Tsubota K, Ozawa Y
  • Journal Title: Biochem Biophys Res Commun Volume: 446 Pages: 535-540
  • DOI: 10.1016/j.bbrc.2014.02.135
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Angiotensin II type 1 receptor blockade suppresses light-induced neural damage in the mouse retina. (2014)
  • Author(s): Narimatsu T, Ozawa Y, Miyake S, Nagai N, Tsubota K.
  • Journal Title: Free Radical Biol Med Volume: 71 Pages: 176-185
  • DOI: 10.1016/j.freeradbiomed.2014.03.020
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Predictive factors for non-response to intravitreal ranibizumab treatment in age-related macular degeneration. (2014)
  • Author(s): Suzuki M, Nagai N, Izumi-Nagai K, Shinoda H, Koto T, Uchida A, Mochimaru H, Yuki K, Sasaki M, Tsubota K
  • Journal Title: British Journal of Ophthalmology Volume: 98 Pages: 1186-1191
  • DOI: 10.1136/bjophthalmol-2013-304670
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Association of Maternal Age to Development and Progression of Retinopathy of Prematurity in Infants of Gestational Age under 33 Weeks. (2014)
  • Author(s): Uchida A, Miwa M, Shinoda H, Koto T, Nagai N, Mochimaru H, Tomita Y, Sasaki M, Ikeda K, Tsubota K, Ozawa Y
  • Journal Title: Journal of Ophthalmology Volume: 2014 Pages: 187929-187929
  • DOI: 10.1155/2014/187929
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Early signs of exudative age-related macular degeneration in Asians. (2014)
  • Author(s): Sasaki M, Kawasaki R, Uchida A, Koto T, Shinoda H, Tsubota K, Tien Yin Wong, Ozawa Y
  • Journal Title: Optom Vis Sci. Volume: 91 Pages: 849-853
  • DOI: 10.1097/opx.0000000000000317
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Resveratrol Prevents the Development of Choroidal Neovascularization by Modulating AMP-activated Protein Kinase in Macrophages and Other Cell Types. (2014)
  • Author(s): Nagai N, Kubota S, Tsubota K, Ozawa Y.
  • Journal Title: J Nutr Biochem. Volume: 25 Pages: 1218-1225
  • DOI: 10.1016/j.jnutbio.2014.05.015
  • Peer Reviewed / Open Access
• [Journal Article] The use of induced pluripotent stem cells to reveal pathogenic gene mutations and explore treatments for retinitis pigmentosa. (2014)
  • Author(s): Yoshida T, Ozawa Y, Suzuki K, Yuki K, Ohyama M, Akamatsu W, Matsuzaki Y, Shimmura S, Mitani K, Tsubota K, Okano H.
  • Journal Title: Mol Brain. Volume: 7 Pages: 45-45
  • DOI: 10.1186/1756-6606-7-45
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Decreased proteasomal activity causes photoreceptor degeneration in mice (2014)
  • Author(s): Ando R, Noda K, Tomaru U, Kamoshita M, Ozawa Y, Notomi S, Hisatomi T, Noda M, Kanda A, Ishibashi T, Kasahara M, Ishida S.
  • Journal Title: Invest. Ophthalmol. Vis. Sci. Volume: 55 Pages: 4682-4690
  • DOI: 10.1167/iovs.13-13272
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] AMPK-NF-κB Axis in the Photoreceptor Disorder during Retinal Inflammation. (2014)
  • Author(s): Kamoshita M, Ozawa Y, Kubota S, Miyake S, Tsuda C, Nagai N, Yuki K, Shimmura S, Umezawa K, Tsubota K.
  • Journal Title: PLOS ONE Volume: 9 Pages: 103013-103013
  • DOI: 10.1371/journal.pone.0103013
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Intraoperative and fluorescein angiographic findings of a secondary macular hole associated with age-related macular degeneration treated by pars plana vitrectomy. (2014)
  • Author(s): Okamoto T, Shinoda H, Kurihara T, Nagai N Tsubota K, Ozawa Y.
  • Journal Title: BMC Ophthalmology. Volume: 14 Pages: 114-114
  • DOI: 10.1186/1471-2415-14-114
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Association of Serum Lipids with Macular Thickness and Volume in Type 2 Diabetes without Diabetic Macular Edema (2014)
  • Author(s): Sasaki M, Kawashima M, Kawasaki R, Uchida A, Koto T, Shinoda H, Tsubota K, Jie Jin Wang, Ozawa Y
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 55 Pages: 1749-1753
  • DOI: 10.1167/iovs.13-13035
  • Peer Reviewed
• [Journal Article] Vitrectomy for myopic foveoschisis with internal limiting membrane peeling and no gas tamponade (2014)
  • Author(s): Uchida A, Shinoda H, Koto T, Mochimaru H, Nagai N, Tsubota K, Ozawa Y
  • Journal Title: Retina Volume: 34 Pages: 455-460
  • DOI: 10.1097/iae.0b013e3182a0e477
  • Peer Reviewed
• [Journal Article] Atp6ap2/(pro)renin receptor interacts with Par3 as a cell polarity determinant required for laminar formation during retinal development in mice. (2013)
  • Author(s): Kanda A, Noda K, Yuki K, Ozawa Y, Furukawa T, Ichihara A & Ishida S
  • Journal Title: J Neurosci. Volume: 33 Pages: 19341-19351
  • DOI: 10.1523/jneurosci.1362-13.2013
  • NAID: 120005438252
  • Peer Reviewed
• [Journal Article] Biological effects of blocking blue and other visible light on the mouse retina. (2013)
  • Author(s): Narimatsu T, Ozawa Y, Miyake S, Kubota S, Yuki K, Nagai N, Tsubota K.
  • Journal Title: Clinical & Experimental Ophthalmology. Volume: [Epub ahead of print] Pages: 1-9
  • DOI: 10.1111/ceo.12253
  • Peer Reviewed
• [Journal Article] ABCA4 Gene Screening by Next-Generation Sequencing in a British Cohort. (2013)
  • Author(s): Fujinami K, Zernant J, Tsunoda K et al.
  • Journal Title: Investigative Ophthalmology & Visual Science Volume: 54(10) Pages: 6662-6674
  • DOI: 10.1167/iovs.13-12570
  • Peer Reviewed
• [Journal Article] Neuroprotective Role of VEGF Induced in Response to Photodynamic Therapy. (2013)
  • Author(s): Ozawa Y, Suzuki M, Niikura N, Kamoshita M, Nagai N, Tsubota K
  • Journal Title: Advances in Neuroimmune Biology Volume: 4 Pages: 161-165
  • DOI: 10.3233/nib-130062
  • Peer Reviewed
• [Journal Article] The Association between Visual Field Defect Severity and Fear of Falling in Primary Open-Angle Glaucoma. (2013)
  • Author(s): Yuki K, Tanabe S, Kouyama K, Fukagawa K, Uchino M, Shimoyama M, Ozeki N, Shiba D, Ozawa Y, Abe T, Tsubota K.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 54 Pages: 7739-7745
  • DOI: 10.1167/iovs.13-12079
  • Peer Reviewed
• [Journal Article] Angiotensin II Type 1 Receptor Antagonist Attenuates Lacrimal Gland, Lung, and Liver Fibrosis in a Murine Model of Chronic Graft-Versus-Host Disease. (2013)
  • Author(s): Yaguchi S, Ogawa Y, Shimmura S, Kawakita T, Hatou S, Satofuka S, Nakamura S, Imada T, Miyashita H, Yoshida S, Yaguchi T, Ozawa Y, Mori T, Okamoto S, Kawakami Y, Ishida S, Tsubota K
  • Journal Title: PLoS ONE. Volume: 8(6)
  • DOI: 10.1371/journal.pone.0064724
  • Peer Reviewed
• [Journal Article] Calorie restriction (CR) and CR mimetics for the prevention and treatment of age-related eye disorders. (2013)
  • Author(s): Kawashima M, Ozawa Y, Shinmura K, Inaba T, Nakamura S, Kawakita T, Watanabe M, Tsubota K.
  • Journal Title: Exp Gerontol. Volume: 48 Pages: 1096-1100
  • DOI: 10.1016/j.exger.2013.04.002
  • Peer Reviewed
• [Journal Article] Blockade for Vascular Adsion Protein-1 Suppresses Pathological Neovascularization in Oxygen-Induced Retinopathy. (2013)
  • Author(s): Yoshikawa N, Noda K, Ozawa Y, Mashima Y, Ishida S.
  • Journal Title: Acta Ophthalmologica. Volume: 91
  • DOI: 10.1111/aos.12128
  • Peer Reviewed
• [Journal Article] Light-dark condition regulates sirtuin mRNA levels in the retina. (2013)
  • Author(s): Ban N, Ozawa Y, Inaba T, Miyake S, Watanabe M, Shinmura K, Tsubota K.
  • Journal Title: Exp Gerontol. Volume: 48 Pages: 1212-1217
  • DOI: 10.1016/j.exger.2013.04.010
  • Peer Reviewed
• [Journal Article] Disruption of cell-cell junctions and induction of pathological cytokines in the retinal pigment epithelium of light-exposed mice. (2013)
  • Author(s): Narimatsu T, Ozawa Y, Miyake S, Kubota S, Hirasawa M, Nagai N, Shimmura S, Tsubota K.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 54 Pages: 4555-4562
  • DOI: 10.1167/iovs.12-11572
  • Peer Reviewed
• [Journal Article] Molecular Characteristics of Four Japanese Cases with KCNV2-retinopathy: Report of Novel Disease-causing Variants. (2013)
  • Author(s): Fujinami K, Tsunoda K, Nakamura N, Kato Y, Noda T, Shinoda K, Tomita K, Hatase T, Usui T, Akahori M, Itabashi T, Iwata T, Ozawa Y, Tsubota K, Miyake Y.
  • Journal Title: Molecular Vision. Volume: 19 Pages: 1580-1590
  • Peer Reviewed
• [Journal Article] Neuroinflammation and Neurodegenerative Disorders of the Retina. (2013)
  • Author(s): Ozawa Y, Kamoshita M, Narimatsu T, Ban N, Toda E, Okamoto T, Yuki K, Miyake S Tsubota K
  • Journal Title: Endocrinology & Metabolic Syndrome. Volume: 2 Pages: 1-4
  • DOI: 10.4172/2161-1017.1000111
  • Peer Reviewed
• [Journal Article] Neuroprotective Role of Superoxide Dismutase 1 in Retinal Ganglion Cells and Inner Nuclear layer Cells against N-methyl-D-aspartate-induced cytotoxicity. (2013)
  • Author(s): Yuki K, Yoshida T, Miyake S, Tsubota K, Ozawa Y
  • Journal Title: Exp Eye Res. Volume: 115 Pages: 230-238
  • DOI: 10.1016/j.exer.2013.07.002
  • Peer Reviewed
• [Journal Article] Detection of early visual impairment in patients with epiretinal membrane. (2013)
  • Author(s): Nishi Y, Shinoda H, Uchida A, Koto T, Mochimaru H, Nagai N, Tsubota K, Ozawa Y.
  • Journal Title: Acta Ophthalmol. Volume: Epub ahead of print Pages: 353-357
  • DOI: 10.1111/aos.12060
  • Peer Reviewed
• [Journal Article] Renin-angiotensin system involvement in the oxidative stress-induced neurodegeneration of cultured retinal ganglion cells. (2013)
  • Author(s): Ozawa Y, Yuki K, Yamagishi R, Tsubota K, Aihara M.
  • Journal Title: Jpn J Ophthalmol. Volume: 57 Pages: 126-32
  • DOI: 10.1007/s10384-012-0204-x
  • NAID: 10031168368
  • Peer Reviewed
• [Journal Article] Serum vascular adhesion protein-1 correlates with vascular endothelial growth factor in patients with type II diabetes (2013)
  • Author(s): Yoshikawa N, Noda K, Shinoda H, Uchida A, Ozawa Y, Tsubota K, Mashima Y, Ishida S.
  • Journal Title: J Diabetes Complications Volume: 27 Pages: 162-6
  • DOI: 10.1016/j.jdiacomp.2012.09.001
  • Peer Reviewed
• [Journal Article] Presence and physiologic function of the Renin-Angiotensin System in mouse lacrimal gland (2012)
  • Author(s): Yaguchi S, Ogawa Y, Shimmura S, Hatou S, Nakamura S, Inaba T, Imada T, Ozawa Y, Kawakami Y, Ishida S, Tsubota K.
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 53 Pages: 5416-25
  • DOI: 10.1167/iovs.12-9891
  • Peer Reviewed
• [Journal Article] Photo-damage mechanisms and anti-apoptotic effect of lutein in the mouse retina. (2012)
  • Author(s): Miyake S, Sasaki M, Takahashi N, Tsubota K, Ozawa Y.
  • Journal Title: Inflammation and Regeneration. Volume: 32 Pages: 208-12
  • NAID: 130004482301
  • Peer Reviewed
• [Journal Article] Soluble vascular adhesion protein-1 accumulates in proliferative diabetic retinopathy (2012)
  • Author(s): Murata M, Noda K, Fukuhara J, Kanda A, Kase S, Saito W, Ozawa Y, Mochizuki S, Kimura S, Mashima Y, Okada Y, Ishida S.
  • Journal Title: Investigative Ophthalmology & Visual Science Volume: 53 Pages: 4055-4062
  • DOI: 10.1167/iovs.12-9857
  • Peer Reviewed
• [Journal Article] Blockade of vascular adhesion protein-1 attenuates choroidal neovascularization. (2012)
  • Author(s): Yoshikawa N, Noda K, Ozawa Y, Tsubota K, Mashima Y, Ishida S.
  • Journal Title: Mol Vis. Volume: 18 Pages: 593-600
  • NAID: 120005372295
  • Peer Reviewed
• [Journal Article] The formation of an angiogenic astrocyte template is regulated by the neuroretina in a HIF-1-dependent manner. (2012)
  • Author(s): Nakamura-Ishizu A, Kurihara T, Okuno Y, Ozawa Y, Kishi K, Goda N, Tsubota K, Okano H, Suda T, Kubota Y
  • Journal Title: Dev Biol. Volume: 363 Pages: 106-114
  • DOI: 10.1016/j.ydbio.2011.12.027
  • Peer Reviewed
• [Journal Article] Vision preservation during retinal inflammation by anthocyanin-rich bilberry extract: cellular and molecular mechanism. (2012)
  • Author(s): Miyake S, Takahashi N, Sasaki M, Kobayashi S, Tsubota K, Ozawa Y
  • Journal Title: Lab Invest. Volume: 92(1) Pages: 102-9
  • DOI: 10.1038/labinvest.2011.132
  • Peer Reviewed
• [Journal Article] Biological role of lutein in the light-induced retinal degeneration. (2012)
  • Author(s): Sasaki M, Yuki K, Kurihara T, Miyake S, Noda K, Kobayashi S, Ishida S, Tsubota K, Ozawa Y.
  • Journal Title: J Nutr Biochem. Volume: 23 Pages: 423-429
  • DOI: 10.1016/j.jnutbio.2011.01.006
  • Peer Reviewed
• [Journal Article] Use of micronutrient supplement for preventing advanced Age-related Macular Degeneration in Japan (2012)
  • Author(s): Sasaki M, Shinoda H, Koto T, Uchida A, Tsubota K, Ozawa Y
  • Journal Title: Archives of Ophthalmology Volume: 130(2) Pages: 254-5
  • DOI: 10.1001/archopthalmol.2011.1368
  • Peer Reviewed
• [Journal Article] Acute visual field defect following vitrectomy determined to originate from optic nerve by electrophysiological tests. (2012)
  • Author(s): Uchida A, Shinoda K, Matsumoto C.S, Kawai M, Kawai S, Ohde H, Ozawa Y, Ishida S, Inoue M, Mizota A, Tsubota K.
  • Journal Title: Case Rep Ophthalmol. Volume: 3 Pages: 396-405
  • DOI: 10.1159/000345507
  • Peer Reviewed
• [Journal Article] Neuroprotective Effects of Lutein in the Retina. (2012)
  • Author(s): Ozawa Y, Sasaki M, Takahashi N, Kamoshita M, Miyake S, Tsubota K
  • Journal Title: Current Pharmaceutical Design. Volume: 18(2) Pages: 51-6
  • DOI: 10.2174/138161212798919101
  • Peer Reviewed
• [Presentation] Management of AMD; anti-VEGF and anti-oxidant approaches. (2014)
  • Author(s): Ozawa Y
  • Organizer: Autumn meeting of Taiwan Ophthalmology Academy
  • Place of Presentation: Chiayi, Taiwan
  • Year and Date: 2014-10-05
  • Invited
• [Presentation] Oxidative stress in the retina and the pathogenesis (2014)
  • Author(s): Ozawa Y
  • Organizer: Inflammatory mechanisms/oxidative stress
  • Place of Presentation: ISOHK-APGC, Hong Kong,
  • Year and Date: 2014-09-26
  • Invited
• [Presentation] Circadian Regulation of Sirtuins in the Eye. Education Course. (2014)
  • Author(s): Ozawa Y
  • Organizer: The Association for Research in Vision and Ophthalmology
  • Place of Presentation: Orland, FL, USA
  • Year and Date: 2014-05-04 – 2014-05-08
  • Invited
• [Presentation] Renin-Angiotensin System (RAS) involvement in the Oxidative Stress-Induced Impairment of Retinal Neuronal Cells. (2013)
  • Author(s): Ozawa Y
  • Organizer: 3rd International Conference on Clinical & Experimental Ophthalmology,
  • Place of Presentation: Chicago/Northbrook, USA
  • Invited
• [Presentation] Disruption of cell-cell junctions and induction of pathological cytokines in the retinal pigment epithelium of light-exposed mice. (2013)
  • Author(s): Ozawa Y,Narimatsu T, Kazuo T.
  • Organizer: 1st International Symposium of Blue Light Society
  • Place of Presentation: JP TOWER Hall & Conference , Marunouchi, Chiyoda-ku, Tokyo
  • Invited
• [Presentation] Disruption of cell-cell junctions and induction of pathological cytokines in the retinal pigment epithelium of light-exposed mice. (2013)
  • Author(s): Ozawa Y
  • Organizer: INTERNATIONAL RETINA SUMMIT
  • Place of Presentation: Hong Kong
  • Invited
• [Presentation] Biological effects of blocking blue and over visible light on the mouse retina. (2013)
  • Author(s): Narimatsu T, Ozawa Y, Miyake S, Kubota S, Yuki K, Nagai N, Tsubota K.
  • Organizer: 1st International Symposium of Blue Light Society
  • Place of Presentation: JP TOWER Hall & Conference , Marunouchi, Chiyoda-ku, Tokyo
• [Presentation] Involvement of Angiopoietin-like 2 in Laser-induced Choroidal Neovascularization. (2013)
  • Author(s): Hirasawa M, Endo M, Kamoshita M, Miyake S, Narimatsu T, Nagai N, Tsubota K, Ozawa Y
  • Organizer: Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting
  • Place of Presentation: Washington State Convention & Trade Center Seattle
• [Presentation] Associations of Serum Lipids with Macular Morphology in Patients without Diabetic Macular Edema. (2013)
  • Author(s): Sasaki M, Kawashima M, Kawasaki R, Kawai M, Uchida A, Koto T, Mochimaru H, Shinoda H, Jie Jin Wang, Tsubota K, Ozawa Y.
  • Organizer: Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting
  • Place of Presentation: Washington State Convention & Trade Center Seattle
• [Presentation] Light-dark Condition Regulates Sirtuin Genes in the Retina. (2013)
  • Author(s): Ban N, Inaba T, Miyake S, Tsubota K, Ozawa Y.
  • Organizer: Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting
  • Place of Presentation: Washington State Convention & Trade Center Seattle
• [Presentation] Baseline characteristics and response to intravitreal ranibizumab therapy for age-related macular degeneration. (2013)
  • Author(s): Suzuki M, Nagai N, Izumi-Nagai K, Shinoda H, Koto T, Uchida A, Mochimaru H, Yuki K, Tsubota K, Ozawa Y
  • Organizer: Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting
  • Place of Presentation: Washington State Convention & Trade Center Seattle
• [Presentation] AMPK-NF-kB axis modulates visual function during inflammation. (2013)
  • Author(s): Kamoshita M, Kubota S, Yuki K, Miyake S, Nagai N, Umezawa K, Tsubota K, Ozawa Y.
  • Organizer: Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting
  • Place of Presentation: Washington State Convention & Trade Center Seattle
• [Presentation] Disruption of cell-cell junctions and induction of pathological cytokines in the retinal pigment epithelium of light-exposed mice. (2013)
  • Author(s): Narimatsu T, Ozawa Y, Miyake S, Kubota S, Hirasawa M, Nagai N, Shimmura S, Tsubota K.
  • Organizer: Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting
  • Place of Presentation: Washington State Convention & Trade Center Seattle
• [Presentation] Evaluation of functional visual acuity in advanced age-related macular degeneration with good visual acuity. (2013)
  • Author(s): Tomita Y, Nagai N, Suzuki M, Sasaki M, Tsubota K, Ozawa Y
  • Organizer: Asia Pacific Vitreo-retina Society Congress
  • Place of Presentation: 名古屋国際会議場 (愛知県名古屋市)
• [Presentation] 脈絡膜新生血管モデルにおける Angiopoietin-like 2の関与 (2013)
  • Author(s): 平沢学, 遠藤元誉, 鴨下衛, 成松俊雄, 三宅誠司, 永井紀博, 坪田一男, 尾池雄一, 小沢洋子
  • Organizer: 第117回 日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム (東京都千代田区)
• [Presentation] AMP活性化プロテインキナーゼによる抗炎症作用と視機能維持 (2013)
  • Author(s): 鴨下衛, 久保田俊介, 三宅誠司, 結城賢弥, 永井紀博, 梅澤一夫, 坪田一男, 小沢洋子.
  • Organizer: 第117回 日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム (東京都千代田区)
• [Presentation] AMP活性化プロテインキナーゼ経路を介した実験的脈絡膜新生血管の抑制 (2013)
  • Author(s): 永井紀博，久保田俊介，坪田一男，小澤洋子
  • Organizer: 第117回 日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム (東京都千代田区)
• [Presentation] 狭義加齢黄斑変性に対するpro re nateによるラニビズマブ硝子体注射. (2013)
  • Author(s): 鈴木美砂, 永井紀博, 永井香奈子, 篠田肇, 内田敦郎, 厚東隆志, 持丸博史, 富田洋平, 佐々木真理子, 坪田一男, 小沢洋子.
  • Organizer: 第67回日本臨床眼科学会
  • Place of Presentation: パシフィコ横浜 (神奈川県横浜市)
• [Presentation] 抗VEGF療法に反応不良の滲出型加齢黄斑変性に対するアフリベルセプトの作用． (2013)
  • Author(s): 永井紀博，永井香奈子，鈴木美砂，持丸博史，富田洋平，内田敦郎，佐々木真理子，篠田肇，坪田一男，小沢洋子
  • Organizer: 第67回日本臨床眼科学会
  • Place of Presentation: パシフィコ横浜 (神奈川県横浜市)
• [Presentation] iPS細胞を用いた網膜色素変性症における視細胞の変性機構の解析 (2013)
  • Author(s): 吉田哲、小沢洋子、鈴木啓一郎、平林由香、鈴木禎史、小泉春菜、結城賢弥、小林哲郎、大山学、天谷雅行、岡田洋平、赤松和土、松崎有未、三谷幸之介、榛村重人、坪田一男、岡野栄之
  • Organizer: 第12回 日本再生医療学会
  • Place of Presentation: 神奈川県横浜市
• [Presentation] An analysis of the mechanisms of degradation of photoreceptor cells in a retinitis pigmentosa patient using iPS cells. (2012)
  • Author(s): Yoshida T, Ozawa Y, Hirabayashi Y, Suzuki K, Mitani K, Kobayashi T, Ohyama M, Amagai M, Okada Y, Akamatsu W, Tsubota K, Shimmura S, Okano H.
  • Organizer: International Society for Stem Cell Research (ISSCR) 10th annual meeting,
  • Place of Presentation: 神奈川県横浜市
• [Presentation] An analysis of the mechanisms of degradation of photoreceptor cells in a retinitis pigmentosa patient using iPS cells. (2012)
  • Author(s): Yoshida T, Ozawa Y, Suzuki K, Hirabayashi Y, Suzuki S, Koizumi H, Yuki K, Kobayashi T, Ohyama M, Amagai M, Okada Y, Akamatsu W, Matsuzaki Y, Mitani K, Shimmra S, Tsubota K, Okano H.
  • Organizer: Global COE Final Symposium .Center for Integrated Medical Research,
  • Place of Presentation: 東京都新宿区