Establishment of lacrimal gland epithelial cell line for the evaluation of lacrimal gland cells

• Project/Area Number: 24592650
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Keio University
• Principal Investigator: KAWAKITA TETSUYA 慶應義塾大学, 医学部, 講師 (50408308)
• Research Collaborator: RYU In 慶應義塾大学, 医学部 ; IKEURA Kazuhiro 慶應義塾大学, 医学部 ; SOMA Yoshio 慶應義塾大学, 医学部, 准教授 (60268183)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 涙腺 / 細胞株 / 再生 / 上皮細胞 / ドライアイ

Outline of Final Research Achievements

We established lacrimal gland epithelial cell line spontaneously from 7-week old CD1 mice. Cyst-like cell aggregates were generated with modifying condition of cultivated time, culture medium, and enzyme treating time. using the defined conditions form established cell line and primary culture. Those cyst were used to observe and analyze the water movement by Coherent Anti-Stokes Raman Scattering microscope. Although water movement could be observed, we need to generate cysts with the same size morphology for applying assay as the function of lacrimal glands. We need further study to solve this problems.