The effect of Mast cell Tryptase to the Expression of Eotaxin-1/CCL11, Eotaxin-2/CCL24 and Eotaxin-3/CCL26 by Conjunctival Fibroblasts.
| Project/Area Number |
24592653
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Akira 順天堂大学, 医学部, 准教授 (00312348)
FUNAKI Toshinari 順天堂大学, 医学部, 准教授 (80384121)
MURAKAMI Akira 順天堂大学, 医学部, 教授 (90157743)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Keywords | 痒み（itch） / トリプターゼ（tryptase） / エオタキシン（eotaxin） / 結膜線維芽細胞 / 春季カタル / Nc/Ngaマウス / トリプターゼ / エオタキシン / 蛋白分解酵素 / 痒み / 好酸球 / 好中球 / IL-8 / アレルギー性結膜炎 |
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| Outline of Final Research Achievements |
To investigate the effect of mast cell tryptase to the expression of eotaxin-1, eotaxin-2 and eotaxin-3 by conjunctival fibroblasts. The production of eotaxin-1, -2 and -3 by conjunctival fibroblasts stimulated with or without IL-4/IL-13 or/and TGF-β1 was estimated by ELISA and PCR. Conjunctival fibroblasts produced eotaxin-1 and -3, but not eotaxin-2. Stimulation with IL-4/IL-13 and TGF-β1 synergistically increased eotaxin-1 and -3 productions. Tryptase reduced the immunoreactivity of eotaxin-1 and -3, but not eotaxin-2, due to the proteolysis of these eotaxins, but not the inhibition of its m-RNA expression. Mast cell tryptase decreases the eotaxin-1 and -3 produced by conjunctival fibroblasts, resulting the inhibition for the excessive accumulation of eosinophils in the conjunctiva. Therefore, eotaxin-2 in the tear may be a biomarker of severity of allergic conjunctival disease.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Therapeutic effects of 0.1% tacrolimus eye drops for refractory allergic ocular diseases with proliferative lesion or corneal involvement.2014
Author(s)
Fukushima A, Ohashi Y, Ebihara N, Uchio E, Okamoto S, Kumagai N, Shoji J, Takamura E, Nakagawa Y, Namba K, Fujishima H, Miyazaki D
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Journal Title
Br J Ophthalmol.
Volume: 98
Issue: 8
Pages: 1023-1027
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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