| Project/Area Number |
24592653
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Akira 順天堂大学, 医学部, 准教授 (00312348)
FUNAKI Toshinari 順天堂大学, 医学部, 准教授 (80384121)
MURAKAMI Akira 順天堂大学, 医学部, 教授 (90157743)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Keywords | 痒み（itch） / トリプターゼ（tryptase） / エオタキシン（eotaxin） / 結膜線維芽細胞 / 春季カタル / Nc/Ngaマウス / トリプターゼ / エオタキシン / 蛋白分解酵素 / 痒み / 好酸球 / 好中球 / IL-8 / アレルギー性結膜炎 |
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| Outline of Final Research Achievements |
To investigate the effect of mast cell tryptase to the expression of eotaxin-1, eotaxin-2 and eotaxin-3 by conjunctival fibroblasts. The production of eotaxin-1, -2 and -3 by conjunctival fibroblasts stimulated with or without IL-4/IL-13 or/and TGF-β1 was estimated by ELISA and PCR. Conjunctival fibroblasts produced eotaxin-1 and -3, but not eotaxin-2. Stimulation with IL-4/IL-13 and TGF-β1 synergistically increased eotaxin-1 and -3 productions. Tryptase reduced the immunoreactivity of eotaxin-1 and -3, but not eotaxin-2, due to the proteolysis of these eotaxins, but not the inhibition of its m-RNA expression. Mast cell tryptase decreases the eotaxin-1 and -3 produced by conjunctival fibroblasts, resulting the inhibition for the excessive accumulation of eosinophils in the conjunctiva. Therefore, eotaxin-2 in the tear may be a biomarker of severity of allergic conjunctival disease.
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