Effect of Tear Hyperosmolarity on Dry eye disease
| Project/Area Number |
24592658
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
FUJIMOTO Chiaki 日本医科大学, 医学(系)研究科(研究院), 研究員 (70623924)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Hiroshi 日本医科大学, 大学院医学研究科, 教授 (00188046)
IGARASGI Tsutomu 日本医科大学, 医学部, 講師 (10421190)
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| Research Collaborator |
KOBAYASHI Maika
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ドライアイ / 涙液浸透圧 / IL-6 / 高浸透圧 / 角膜上皮細胞 / 炎症 / サイトカイン / 浸透圧 / 眼表面 / TLR / 結膜上皮細胞 / Th17 |
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| Outline of Final Research Achievements |
To examine the effect of short-time hyperosmolarity exposure in the production of inflammatory cytokines in corneal epithelial cells, Human Corneal Cells (HCE) cells were used in vitro.
After a 24-hour culture, exposures over 500 mOsm damaged HCE cells, but 400 mOsm caused no cell damage. In contrast, after the short-time (10-minute) exposures, even when at over 800 mOsm, no cells was damaged. The significant increases in IL-6 production and mRNA expression at 700 mOsm during the short-time exposures were both dependent on the osmolarity.
Short-time hyperosmolarity exposure may activate IL-6 expression and production in Human Corneal Epithelial cells without cytotoxicity. These observations suggest that hyperosmolarity could cause inflammation on the ocular surface in dry eye disease.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Rapid and Transient Upregulation of CCL11 (Eotaxin-1) in Mouse Ovary During Terminal Stages of Follicular Development.2013
Author(s)
Kuwabara Y, Katayama A, Igarashi T, Tomiyama R, Piao H, Kaneko R, Abe T, Mine K, Akira S, Orimo H, Takeshita T.
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Journal Title
Am J Reprod Immunol.
Volume: 67
Pages: 358-68
Related Report
Peer Reviewed
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[Journal Article] A new mutation in RP1L1 gene in occult macular dystrophy patient associated with a depolarizing-pattern of focal macular ERG.2012
Author(s)
Kabuto T, Takahashi H, Goto-Fukuura Y, Igarashi T, Akahori M, Kameya S, Iwata T, Mizota A, Yamaki K, Miyake Y, Takahashi H.
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Journal Title
Molecular Vision.
Volume: 18
Pages: 1031-9
Related Report
Peer Reviewed
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