| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
For neurotrophic keratopathy in eyes with keratoplasty or LASIK, we will generate a new treatment of corneal nerve regenerative induction.
We have tried to develop three dimensional gel culture system which can carry out quantitative estimation constantly. Until now, co-culture of neural cells (PC12) and corneal fibroblasts in three dimensional gel have been successful. However, nerve fiber induction rate by gradient concentration of the nerve growth factor (NGF) have been unstable. On the other hand, with co-culture system by vitrigel, trigeminal nerve is essential for corneal epithelial stratification. Furthermore, our results revealed that substance P and CGRP play important role for corneal epithelial stratification.
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