| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
It is considered that the clinical efficacy of regenerative medicine of corneal epithelium is improved by use of corneal epithelial cell sheet constructed by transdifferentiation of autologous epidermal cells. In this study, culture method of transdifferentiated cells from mouse epidermal cells (MECs) or side population (sp) cells was examined. And, to examine the factors that have possibility to influence the transdifferentiation, transcriptome analysis was performed for mouse limbal stromal fibroblasts (MLSFs). When MECs or sp cells were cultured on limbal stroma or MLSF feeder cells, K12 expression was observed in the epithelial cells. The transcriptome analysis revealed the high-level expression of some Wnt inhibitors. Further study is necessary to reveal the role of these factors on the transdifferentiation.
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