Retinal stem cell in the pathogenesis of macular diseases
| Project/Area Number |
24592685
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Osaka Medical College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKU Hidehiro 大阪医科大学, 医学部, 准教授 (90177163)
SUGIYAMA Tetsuya 大阪医科大学, 医学部, 非常勤講師 (20298764)
KURIMOTO Takuji 大阪医科大学, 医学部, 助教 (50388815)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKAI Shinji 大阪医科大学, 医学部, 教授 (80288703)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 網膜幹細胞 / 遺伝子発現 / 黄斑部 / リアルタイムPCR / 黄斑円孔 / 黄斑上膜 / 遺伝子 |
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| Outline of Final Research Achievements |
Based on the hypothesis that undifferentiated retinal stem cell (RSC)-like cells exist in the fovea, we investigated the expression sites of neural stem cell (NSC)-related genes in the monkey retina. Flat-mounted retina samples were prepared using cynomolgus monkeys eye. 1-mm x 1-mm blocks of the retina at the fovea, mid-periphery, and extreme periphery were excised. These samples were used for real-time polymerase chain reaction analysis of the NSC-related gene (nestin, PAX6, and SOX2) expression at each site. Nestin expression was high in the fovea, with a lower expression in the mid-periphery and extreme periphery. No differences in PAX6 gene expression were found in the fovea, mid-periphery, and extreme periphery. SOX2 expression was highest in the extreme periphery, with decreased expression in the mid-periphery and fovea. Our finding that nestin expression was highest in the fovea suggests that foveal retinal cells may be related to the onset of macular diseases
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Report
(4 results)
Research Products
(9 results)
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[Presentation] The role of tryptase and anti-type II collagen antibodies in the pathogenesis of idiopathic epiretinal membranes2015
Author(s)
Tsunehiko Ikeda, Kimitoshi Nakamura, Hidehiro Oku, Seita Morishita, Masanori Fukumoto, Hiroyuki Suzuki, Teruyo Kida, Taeko Horie, Tetsuya Sugiyama, Shinji Takai
Organizer
2015 ARVO Annual Meeting
Place of Presentation
Colorado Cnvention Center(Colorado,USA)
Year and Date
2015-05-03 – 2015-05-07
Related Report
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[Presentation] 糖尿病網膜症と自己免疫2014
Author(s)
池田恒彦
Organizer
第16回 Japan Macula Club
Place of Presentation
蒲郡クラシックホテル（愛知県蒲郡市）
Year and Date
2014-08-23 – 2014-08-24
Related Report
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