| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We developed a protocol to consistently and efficiently differentiate 3D-retina by self-organization from nrl-GFP miPS cell and rx-GFP mES cell lines. We observed that the differentiated retinal tissue of dd17 or younger can efficiently developed structured photoreceptor layer (or outer nuclear layer, ONL) after transplantation and integrate onto the inner layer of host retina. Photoreceptors in these structured ONL showed a mature photoreceptor marker and, by electron microscopic observation, developed a characteristic component of photoreceptors, outer segments. Furthermore, synaptic contact between host bipolar cells and graft photoreceptor cells were also suggested by immune-histochemistry. Functional studies by electrophysiology and behavior test are currently underway.
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