| Project/Area Number |
24592692
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Chiba University |
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Principal Investigator |
SAITO Takeshi 千葉大学, 医学(系)研究科(研究院), 講師 (20406044)
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| Co-Investigator(Kenkyū-buntansha) |
HATANO Masahiko 千葉大学, 大学院医学研究院, 教授 (20208523)
SAKAMOTO Akemi 千葉大学, 大学院医学研究院, 助教 (90359597)
YOSHIDA Hideo 千葉大学, 大学院医学研究院, 教授 (60210712)
MITSUNAGA Tetsuya 千葉大学, 医学部附属病院, 助教 (80375774)
NAKATA Mitsuyuki 千葉大学, 医学部附属病院, 助教 (90375775)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 制御性T細胞 / 胆道閉鎖症 / サイトカイン / 炎症制御 / 免疫学 / 小児肝胆道疾患 |
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| Outline of Final Research Achievements |
Relationship of Th17 and Treg in the systemic and local immune environment of biliary atresia (BA) was investigated. Significantly lower frequency of Treg (CD4+CD25+) population in the preoperative systemic circulation, higher mRNA expressions of FoxP3, representative Treg transcriptional factor, in the liver tissue, and the focal distribution of FoxP3 mRNA centering on the portal area, in patients with BA, showed that Treg migrated from the systemic circulation into the liver, especially portal area, demonstrating the possible involvement of Treg in the development and progression of BA. However, there was no evidence for significantly activated Th17-related immunological loop based on the results from plasma concentration analysis by flowcytometry and hepatic mRNA expressions by real-time PCR, concerning Th17 representative cytokines, negating the Th17-related proinflammatory process. Moreover, Treg suppression assay showed the comparatively lower Treg functions in progressive BA.
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