Improvement of ischemia reperfusion injury after liver transplantation by introduction of MSCs

• Project/Area Number: 24592694
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatric surgery
• Research Institution: Kyoto University
• Principal Investigator: OKAMOTO Shinya 京都大学, 医学(系)研究科(研究院), 研究員 (00329377)
• Co-Investigator(Kenkyū-buntansha): 上本 伸二 京都大学, 医学(系)研究科(研究院), 教授 (40252449) ; 寺谷 工 自治医科大学, 医学部, 講師 (70373404) ; 藤本 康弘 京都大学, 医学(系)研究科(研究院), 助教 (80335281)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: MSCs / exozome / 肝移植 / 部分肝移植 / 小腸移植 / 臓器保存 / 間葉系幹細胞 / exosome

Outline of Final Research Achievements

We have shown that mesenchymal stem cells administered via portal vein is effective in terms of amelioration of ischemia-reperfusion injury. We also established the technique with which we can extract exosome from supernatant of culture media of MSCs. Exosome from MSCs was shown to have immuno-moduratory effect and suppress immunological reaction between allogeneic cells. In order to evaluate the effect of exosome we esbalished rat liver transplantation model. The tentative results showed that the exosome have a potential to improve rat survival after partial liver transplantation.

Research Products

• [Journal Article] High prevalence of carbapenem resistance among plasmid-mediated AmpC β-lactamase-producing Klebsiella pneumoniae during outbreaks in liver transplantation units. (2015)
  • Author(s): Matsumura Y, Tanaka M, Yamamoto M, Nagao M, Machida K, Ito Y, Takakura S, Ogawa K, Yoshizawa A, Fujimoto Y, Okamoto S, Uemoto S, Ichiyama S.
  • Journal Title: Int J Antimicrob Agents. Volume: 45 Issue: 1 Pages: 33-40
  • DOI: 10.1016/j.ijantimicag.2014.08.015
  • Peer Reviewed
• [Journal Article] Prognostic significance of Aminopeptidase-N (CD13) in hepatoblastoma (2015)
  • Author(s): Saida S, Watanabe KI, Kato I, Fujino H, Umeda K, Okamoto S, Uemoto S, Hishiki T, Yoshida H, Tanaka S, Adachi S, Niwa A, Nakahata T, Heike T
  • Journal Title: Pediatrics International Volume: in press Issue: 4 Pages: 558-566
  • DOI: 10.1111/ped.12597
  • Peer Reviewed
• [Journal Article] Long-term outcome of percutaneous transhepatic balloon angioplasty for portal vein stenosis after pediatric living donor liver transplantation: a single institute's experience. (2014)
  • Author(s): Yabuta M, Shibata T, Shibata T, Shinozuka K, Isoda H, Okamoto S, Uemoto S, Togashi K.
  • Journal Title: J Vasc Interv Radiol. Volume: 25 Issue: 9 Pages: 1406-1412
  • DOI: 10.1016/j.jvir.2014.03.034
  • Peer Reviewed
• [Journal Article] Impact of cytochrome P450 3A5 polymorphism in graft livers on the frequency of acute cellular rejection in living-donor liver transplantation. (2014)
  • Author(s): Uesugi M, Kikuchi M, Shinke H, Omura T, Yonezawa A, Matsubara K, Fujimoto Y, Okamoto S, Kaido T, Uemoto S, Masuda S.
  • Journal Title: Pharmacogenet Genomics. Volume: 24 Issue: 7 Pages: 356-366
  • DOI: 10.1097/fpc.0000000000000060
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of liver preservation solutions by using rats transgenic for luciferase (2014)
  • Author(s): Doi, J. Teratani, T. Kasahara, N. Kikuchi, T. Fujimoto, Y. Uemoto, S. Kobayashi, E.
  • Journal Title: Transplant Proc Volume: 46 Issue: 1 Pages: 63-65
  • DOI: 10.1016/j.transproceed.2013.07.077
  • Peer Reviewed
• [Journal Article] Portocaval shunt for hepatocyte package: challenging application of small intestinal graft in animal models (2013)
  • Author(s): Iwasaki, J. Hata, T. Uemoto, S. Fujimoto, Y. Kanazawa, H. Teratani, T. Hishikawa, S. Kobayashi, E.
  • Journal Title: Organogenesis Volume: 9 Issue: 4 Pages: 273-9
  • DOI: 10.4161/org.25968
  • Peer Reviewed
• [Journal Article] Suppression of acute rejection by administration of prostaglandin E2 receptor subtype 4 agonist in rat organ transplantation models (2013)
  • Author(s): Okamoto T, Okamoto S, Fujimoto Y, Tabata Y, Uemoto S.
  • Journal Title: J Surg Res. Volume: S0022 Pages: 4804-8
  • Peer Reviewed
• [Journal Article] Prevention of trinitrobenzene sulfonic acid-induced experimental colitis by oral administration of a poly(lactic-coglycolic acid) microsphere containing prostaglandin E₂ receptor subtype 4 agonist. (2012)
  • Author(s): Okamoto T, Uemoto S, Tabata Y
  • Journal Title: J Pharmacol Exp Ther. Volume: 341 Pages: 340-9
  • Peer Reviewed
• [Presentation] 当院で経験した先天性横隔膜ヘルニアの成績 (2014)
  • Author(s): 岡本 晋弥
  • Organizer: 第５１回日本小児外科学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2014-05-15