Development of new therapy for congenital metabolic disorders using functional three-dimensional liver structures constructed by stem cells from human exfoliated deciduous teeth
| Project/Area Number |
24592696
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
YANAGI Yusuke 九州大学, 医学(系)研究科(研究院), 研究員 (30596664)
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| Co-Investigator(Kenkyū-buntansha) |
TAGUCHI Tomoaki 九州大学, 医学(系)研究科(研究院), 教授 (20197247)
NAKAYAMA Koichi 佐賀大学, 工学(系)研究科(研究院), 教授 (50420609)
HAYASHIDA Makoto 九州大学, 大学病院, 助教 (70452761)
KOHASHI Kenichi 九州大学, 大学病院, 講師 (10529879)
IHARA Kenji 大分大学, 医学(系)研究科(研究院), 教授 (80294932)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAZA Takayoshi 九州大学, 歯学(系)研究科(研究院), 講師 (80304814)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 歯髄幹細胞 / 肝細胞移植 / 肝組織工学 / 代謝性肝疾患 / 人工臓器 / 再生医療 / 再生医学 / 先天性肝疾患 / 乳歯歯髄幹細胞 |
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| Outline of Final Research Achievements |
We successfully differentiated stem cells from Human Exfoliated Deciduous teeth(SHED) to hepatocyte-like cells by our culture system. SHED derived hepatocyte-like cells(SHED-Hep) showed low immunogenicity and suppression of T cells in vitro. Following these result, we xenografted SHED-Hep into carbon tetrachloride (CCl4)-induced chronic liver fibrosis model mice without immunosuppressive therapy, showing the engraftment in the recipient liver, anti-fibrotic efficacy and prolonged survival. In the next step, we fabricated 3D liver construct with human primary hepatocytes, umbilical vein endothelial cells and mesenchymal stem cells using a bio-3D printer. It’s microstructure resembled real liver tissue in histology, and it showed liver specific functions. We also succeed in biofabrication of 3D liver construct with SHED-Hep. In future, we will transplant this SHED derived construct to develop new liver regenerative therapy.
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Report
(4 results)
Research Products
(11 results)
