| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
We tried to perform the long-term culture in vitro with our gut-like organ differentiation from induced pluripotent stem (iPS) cells. We paid attention to adipose tissue-derived stem cells (ADSC) with ability for angiogenesis, we cultured iPS cells and ADSCs together. We tried to differentiation of intestinal mesentery, we demonstrated that ADSCs expressed the vascular endothelial marker genes such as CD31. However we could not develop fatty tissue with vascular formation by tissue-engineering technique. We tried to angiogenesis for gut-like organ using the embryoid body from iPS cells and ADSCs by three-demensional culture. We could not confirm the vascular formation. We could not demonstrate the angiogenesis with vascular formation for gut-like organ in vitro.
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