| Project/Area Number |
24592700
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Keio University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KURODA Tatsuo 慶應義塾大学, 医学部, 教授 (60170130)
SHIBATE Shinsuke 慶應義塾大学, 医学部, 講師 (70407089)
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| Co-Investigator(Renkei-kenkyūsha) |
OKANO Hideyuki 慶應義塾大学, 医学部, 教授 (60160694)
KUDO Jun 慶應義塾大学, 医学部, 教授 (80178003)
KOSAKI Kenjiro 慶應義塾大学, 医学部, 教授 (30234743)
FUJIMURA Takumi 慶應義塾大学, 医学部, 助教 (80573443)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 腸管神経 / 発生 / 分化 / 幹細胞 / 再生医療 / 神経堤細胞 / ヒルシュスプルング病 / ヒルシュスプルング病類縁疾患 / hypoganglionosis / 腸管グリア / 神経堤幹細胞 / 網羅的遺伝子解析 / 次世代シーケンサー |
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| Outline of Final Research Achievements |
A final goal of our project is to establish a cell transplant therapy for Hirschsprung's disease and allied disorders. Understanding the etiology of diseases is very important, so we began with gene analyses using human samples from patients with hypoganglionosis. To date, we identified 2 candidate genes which might be causative genes.
For a better understanding of etiology of hypoganglionosis, populations of ganglion cells and glial cells were analyzed quantitatively. Immunohistochemical staining for enteric nerves and glial cells were performed. Ganglion cells and glial cells were both decreased in number but the ratio of glial cells to ganglion cells was significantly lower in hypoganglionosis. Thus, the result from a disturbed generation of trophic factors by accompanying glial cells might be an etiological factor for the decreased number of ganglion cells in hypoganglionosis.
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