Functional recovery of visual pathway after vascularized peripheral nerve grafting

• Project/Area Number: 24592714
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: International University of Health and Welfare (2014-2015); Okayama University (2012-2013)
• Principal Investigator: Kosaka Jun 国際医療福祉大学, 保健医療学部, 教授 (40243216)
• Co-Investigator(Kenkyū-buntansha): KIMATA Yoshihiro 岡山大学, 大学院医歯薬学総合研究科, 教授 (50392345) ; WAKABAYASHI Taketoshi 関西医科大学, 医学部, 准教授 (90302421)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 血管柄付き移植 / マイクロサージェリー / 網膜神経節細胞 / 視神経 / 視覚中枢 / 髄鞘

Outline of Final Research Achievements

We studied whether the use of vascularized peripheral nerve graft on the optic nerve stump enhances axonal regrowth of retinal ganglion cells compared with classical non-vascularized grafts. The rat median nerve was microsurgically sutured with its supplying artery and vein to the optic nerve stump. The number of retinal ganglion cells with regrowing axons was evaluated and counted by retrograde labeling into the grafted nerve. Myelination of the regrowing axon fibers was examined by transmitted electron microscopy. The number of retinal ganglion cells with regrowing axons in the vascularized graft was significantly higher than in the classical non-vascularized graft. The ratio of myelinated axon fibers was also increased in vascularized graft. Thus, peripheral nerve grafting with their supplying arteries and veins to the injured nerve axon represents a promising strategy to accelerate axonal regeneration.

Research Products

• [Journal Article] Comparative morphology and development of extra-ocular muscles in the lamprey and gnathostomes reveal the ancestral state and developmental patterns of the vertebrate head. (2016)
  • Author(s): Suzuki D*, Fukumoto Y, Yoshimura M, Yamazaki Y, Kosaka J, Kuratani S, Wada H.
  • Journal Title: Zoological Letters Volume: 2 Issue: 1 Pages: 10-10
  • DOI: 10.1186/s40851-016-0046-3
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Only extra-high dose of ketamine affects L-glutamate-induced intracellular Ca2+ elevation and neurotoxicity. (2015)
  • Author(s): Shibuta S*, Morita T, Kosaka J, Kamibayashi T, Fujio Y.
  • Journal Title: Neuroscience Research Volume: 98 Pages: 9-16
  • DOI: 10.1016/j.neures.2015.04.005
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Sox2 promotes survival of satellite glial cells in vitro. (2015)
  • Author(s): Koike T, Wakabayashi T, Mori T, Hirahara Y, Yamada H.
  • Journal Title: Biochem Biophys Res Commun. Volume: 464(1) Issue: 1 Pages: 269-274
  • DOI: 10.1016/j.bbrc.2015.06.141
  • NAID: 120006778372
  • Peer Reviewed / Open Access
• [Journal Article] Only extyra-high dose of ketamine affects L-glutamate-induced intracellular Ca2+ elevation and neurotoxicity. (2015)
  • Author(s): Shibuta S, Morita T, Kosaka J, Kamibayashi T, Fujino Y.
  • Journal Title: Neuroscience Research Volume: 000 Pages: 000-000
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Organization and cellular arrangement of two neurogenic regions in the adult ferret (Mustela putorius furo) brain. (2014)
  • Author(s): Takamori Y, Wakabayashi T, Mori T, Kosaka J, Yamada H
  • Journal Title: J Comp Neurol. Volume: 522(8) Issue: 8 Pages: 1818-1838
  • DOI: 10.1002/cne.23503
  • Peer Reviewed
• [Journal Article] Vascularized peripheral nerve grafting promotes myelination of regrowing optic nerve. (2013)
  • Author(s): Komatsu S, Wakabayashi T, Matsumoto K, Yamada K, Kimata Y, Kosaka J.
  • Journal Title: NeuroReport Volume: 24(10) Pages: 566-571
  • NAID: 120005359104
  • Peer Reviewed
• [Journal Article] Loss of Ahi1 affects early development by impairing BM88/Cend1-mediated neuronal differentiation (2013)
  • Author(s): Li XJ, Weng L, Ling YF, Li A, Wang CE, Yan S, Sun M, Gaertig M, Mitha N, Kosaka J, Wakabayashi T, Xu X, Tang B, Li S.
  • Journal Title: Journal of Neuroscience Volume: 33 Issue: 19 Pages: 8172-8184
  • DOI: 10.1523/jneurosci.0119-13.2013
  • Peer Reviewed
• [Journal Article] Vascularized peripheral nerve grafting promotes myelination of regrowing optic nerve. (2013)
  • Author(s): Komatsu S, Wakabayashi T, Yamada K, Matsumoto K, Kimata Y, Kosaka J.
  • Journal Title: NeuroReport Volume: 印刷中
  • NAID: 120005359104
  • Peer Reviewed
• [Journal Article] YamadaHProlonged Expression of Puma in Cholinergic Amacrine Cells During the Development of Rat Retina. (2012)
  • Author(s): Wakabayashi T, Kosaka J, Mori T
  • Journal Title: J Histochem Cytochem Volume: 60(10) Issue: 10 Pages: 777-788
  • DOI: 10.1369/0022155412452737
  • Peer Reviewed
• [Presentation] in situハイブリダイゼーション組織化学法の標準化(P-M-17) (2015)
  • Author(s): 小阪淳
  • Organizer: 国際医療福祉大学学会
  • Place of Presentation: 国際医療福祉大学大田原キャンパス
  • Year and Date: 2015-08-30
• [Presentation] ラット網膜発生過程でのBH3-only proteinの一種Pumaの発現パターン (2014)
  • Author(s): 若林毅俊。小阪淳、森徹自、山田久夫
  • Organizer: 第119回日本解剖学会総会
  • Place of Presentation: 自治医科大学キャンパス
• [Presentation] 栄養血管付き末梢神経移植による視神経再生の促進 (2013)
  • Author(s): 小阪淳、小松星児、若林毅俊、山田潔、松本久美子、徳山英二郎、杉山成史、木股敬裕
  • Organizer: 第118回日本解剖学会総会
  • Place of Presentation: 香川県高松市
• [Presentation] 血管柄付き正中神経移植によるラット視神経再生の促進と有髄化 (2012)
  • Author(s): 小阪淳、小松星児、若林毅俊、松本久美子、徳山英二郎、山田潔、高橋寛二、山田久夫、木股敬裕
  • Organizer: 第116回日本眼科学会総会
  • Place of Presentation: 東京都千代田区・東京国際フォーラム
• [Remarks] 国際医療福祉大学・基礎医学研究センター・ホームページ
  • URL: http://www.iuhw.ac.jp/gakubu/fundamental.html
• [Remarks] 網膜生物学・視神経再生グループ・ホームページ
  • URL: http://square.umin.ac.jp/oka-anat/kosaka/index.html
• [Remarks] 網膜生物学・視神経再生研究グループ・ホームページ
  • URL: http://square.umin.ac.jp/oka-anat/kosaka/index.html
• [Remarks] 網膜生物学・視神経再生研究グループ・ホームページ（現在リンク切れにて調整中）
  • URL: http://square.umin.ac.jp/oka-anat/kosaka/lab_members/index.html
• [Remarks] 岡山大学大学院医歯薬学総合研究科・細胞組織学ホームページ
  • URL: http://square.umin.ac.jp/oka-anat/index.html