Evidence of epithelial-mesenchymal transition in skin wound healing
Project/Area Number |
24592718
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plastic surgery
|
Research Institution | Jichi Medical University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
SUNAGA Ataru 自治医科大学, 形成外科, 助教 (00406117)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | 創傷治癒 / lineage tracing / 血管内皮間葉移行 / 遺伝子改変マウス / 細胞運命追跡 / 血管内皮間葉転換 |
Outline of Final Research Achievements |
We established Tie2Cre;Ai14;COL-EGFP mice in which Tie2-expressing cells were genetically marked with tdTomato and collagen type1-expressing cells expressed EGFP. Using the transgenic mice, we made hypertrophic scar models and normal scar models, and analyzed the scars with immunofluorescent imaging. In the normal scars, tdTomato-positive cells (tdT-cells) did not express EGFP, suggesting tdT-cells did not produce collagen type 1. In the other hand, tdT-cells in the hypertrophic scars partially expressed EGFP, suggesting tdT-cells contributed to dermal fibrosis.
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Report
(4 results)
Research Products
(2 results)